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Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity

Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, t...

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Autores principales: Li, Yuchen, Aiello, Gilda, Bollati, Carlotta, Bartolomei, Martina, Arnoldi, Anna, Lammi, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146380/
https://www.ncbi.nlm.nih.gov/pubmed/32197331
http://dx.doi.org/10.3390/nu12030794
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author Li, Yuchen
Aiello, Gilda
Bollati, Carlotta
Bartolomei, Martina
Arnoldi, Anna
Lammi, Carmen
author_facet Li, Yuchen
Aiello, Gilda
Bollati, Carlotta
Bartolomei, Martina
Arnoldi, Anna
Lammi, Carmen
author_sort Li, Yuchen
collection PubMed
description Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic activity was assessed by using either biochemical or cellular techniques. Findings suggest that PBP peptides inhibit DPP-IV activity in vitro with a dose-response trend and an IC(50) value falling in the range between 0.5 and 1.0 mg/mL. A lower inhibition of the DPP-IV activity expressed by Caco-2 cells was observed, which was explained by a secondary metabolic degradation exerted by the same cells.
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spelling pubmed-71463802020-04-15 Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity Li, Yuchen Aiello, Gilda Bollati, Carlotta Bartolomei, Martina Arnoldi, Anna Lammi, Carmen Nutrients Article Arthrospira platensis (spirulina) is a cyanobacterium, which contains mainly two phycobiliproteins (PBP), i.e., C-phycocyanin (C-PC) and allophycocyanin (APC). In this study, PBP were hydrolyzed using trypsin, and the composition of the hydrolysate was characterized by HPLC-ESI-MS/MS. Furthermore, the potential anti-diabetic activity was assessed by using either biochemical or cellular techniques. Findings suggest that PBP peptides inhibit DPP-IV activity in vitro with a dose-response trend and an IC(50) value falling in the range between 0.5 and 1.0 mg/mL. A lower inhibition of the DPP-IV activity expressed by Caco-2 cells was observed, which was explained by a secondary metabolic degradation exerted by the same cells. MDPI 2020-03-18 /pmc/articles/PMC7146380/ /pubmed/32197331 http://dx.doi.org/10.3390/nu12030794 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yuchen
Aiello, Gilda
Bollati, Carlotta
Bartolomei, Martina
Arnoldi, Anna
Lammi, Carmen
Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title_full Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title_fullStr Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title_full_unstemmed Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title_short Phycobiliproteins from Arthrospira Platensis (Spirulina): A New Source of Peptides with Dipeptidyl Peptidase-IV Inhibitory Activity
title_sort phycobiliproteins from arthrospira platensis (spirulina): a new source of peptides with dipeptidyl peptidase-iv inhibitory activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146380/
https://www.ncbi.nlm.nih.gov/pubmed/32197331
http://dx.doi.org/10.3390/nu12030794
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