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Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions w...

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Autores principales: Liu, Kai-Hsi, O’Hare, Danny, Thomas, James L., Guo, Han-Zhang, Yang, Chien-Hsin, Lee, Mei-Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146556/
https://www.ncbi.nlm.nih.gov/pubmed/32120922
http://dx.doi.org/10.3390/bios10030016
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author Liu, Kai-Hsi
O’Hare, Danny
Thomas, James L.
Guo, Han-Zhang
Yang, Chien-Hsin
Lee, Mei-Hwa
author_facet Liu, Kai-Hsi
O’Hare, Danny
Thomas, James L.
Guo, Han-Zhang
Yang, Chien-Hsin
Lee, Mei-Hwa
author_sort Liu, Kai-Hsi
collection PubMed
description Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%.
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spelling pubmed-71465562020-04-20 Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone Liu, Kai-Hsi O’Hare, Danny Thomas, James L. Guo, Han-Zhang Yang, Chien-Hsin Lee, Mei-Hwa Biosensors (Basel) Article Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings and functional groups, which can undergo π-π and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted electronically conductive polymers (TIECPs) on sensing electrodes. The linear sensing range for testosterone was from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000-fold to measure testosterone concentration using the above TIECP sensors; results were compared with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33 ± 0.09 to 9.13 ± 1.33 ng/mL. The mean accuracy of the TIECP-coated sensors was 90.3 ± 7.0%. MDPI 2020-02-27 /pmc/articles/PMC7146556/ /pubmed/32120922 http://dx.doi.org/10.3390/bios10030016 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Kai-Hsi
O’Hare, Danny
Thomas, James L.
Guo, Han-Zhang
Yang, Chien-Hsin
Lee, Mei-Hwa
Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title_full Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title_fullStr Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title_full_unstemmed Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title_short Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone
title_sort self-assembly synthesis of molecularly imprinted polymers for the ultrasensitive electrochemical determination of testosterone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146556/
https://www.ncbi.nlm.nih.gov/pubmed/32120922
http://dx.doi.org/10.3390/bios10030016
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