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Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation

The chronic low-grade inflammation in adipose tissue plays a causal role in obesity-induced insulin resistance and its associated pathophysiological consequences. In this study, we investigated the effects of extracts of Broussonetia papyrifera root bark (PRE) and its bioactive components on inflamm...

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Detalles Bibliográficos
Autores principales: Lee, Jae Min, Choi, Sun Sil, Park, Mi Hyeon, Jang, Hyunduk, Lee, Yo Han, Khim, Keon Woo, Oh, Sei Ryang, Park, Jiyoung, Ryu, Hyung Won, Choi, Jang Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146562/
https://www.ncbi.nlm.nih.gov/pubmed/32183397
http://dx.doi.org/10.3390/nu12030773
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author Lee, Jae Min
Choi, Sun Sil
Park, Mi Hyeon
Jang, Hyunduk
Lee, Yo Han
Khim, Keon Woo
Oh, Sei Ryang
Park, Jiyoung
Ryu, Hyung Won
Choi, Jang Hyun
author_facet Lee, Jae Min
Choi, Sun Sil
Park, Mi Hyeon
Jang, Hyunduk
Lee, Yo Han
Khim, Keon Woo
Oh, Sei Ryang
Park, Jiyoung
Ryu, Hyung Won
Choi, Jang Hyun
author_sort Lee, Jae Min
collection PubMed
description The chronic low-grade inflammation in adipose tissue plays a causal role in obesity-induced insulin resistance and its associated pathophysiological consequences. In this study, we investigated the effects of extracts of Broussonetia papyrifera root bark (PRE) and its bioactive components on inflammation and insulin sensitivity. PRE inhibited TNF-α-induced NF-κB transcriptional activity in the NF-κB luciferase assay and pro-inflammatory genes’ expression by blocking phosphorylation of IκB and NF-κB in 3T3-L1 adipocytes, which were mediated by activating AMPK. Ten-week-high fat diet (HFD)-fed C57BL6 male mice treated with PRE had improved glucose intolerance and decreased inflammation in adipose tissue, as indicated by reductions in NF-κB phosphorylation and pro-inflammatory genes’ expression. Furthermore, PRE activated AMP-activated protein kinase (AMPK) and reduced lipogenic genes’ expression in both adipose tissue and liver. Finally, we identified broussoflavonol B (BF) and kazinol J (KJ) as bioactive constituents to suppress pro-inflammatory responses via activating AMPK in 3T3-L1 adipocytes. Taken together, these results indicate the therapeutic potential of PRE, especially BF or KJ, in metabolic diseases such as obesity and type 2 diabetes.
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spelling pubmed-71465622020-04-20 Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation Lee, Jae Min Choi, Sun Sil Park, Mi Hyeon Jang, Hyunduk Lee, Yo Han Khim, Keon Woo Oh, Sei Ryang Park, Jiyoung Ryu, Hyung Won Choi, Jang Hyun Nutrients Article The chronic low-grade inflammation in adipose tissue plays a causal role in obesity-induced insulin resistance and its associated pathophysiological consequences. In this study, we investigated the effects of extracts of Broussonetia papyrifera root bark (PRE) and its bioactive components on inflammation and insulin sensitivity. PRE inhibited TNF-α-induced NF-κB transcriptional activity in the NF-κB luciferase assay and pro-inflammatory genes’ expression by blocking phosphorylation of IκB and NF-κB in 3T3-L1 adipocytes, which were mediated by activating AMPK. Ten-week-high fat diet (HFD)-fed C57BL6 male mice treated with PRE had improved glucose intolerance and decreased inflammation in adipose tissue, as indicated by reductions in NF-κB phosphorylation and pro-inflammatory genes’ expression. Furthermore, PRE activated AMP-activated protein kinase (AMPK) and reduced lipogenic genes’ expression in both adipose tissue and liver. Finally, we identified broussoflavonol B (BF) and kazinol J (KJ) as bioactive constituents to suppress pro-inflammatory responses via activating AMPK in 3T3-L1 adipocytes. Taken together, these results indicate the therapeutic potential of PRE, especially BF or KJ, in metabolic diseases such as obesity and type 2 diabetes. MDPI 2020-03-14 /pmc/articles/PMC7146562/ /pubmed/32183397 http://dx.doi.org/10.3390/nu12030773 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jae Min
Choi, Sun Sil
Park, Mi Hyeon
Jang, Hyunduk
Lee, Yo Han
Khim, Keon Woo
Oh, Sei Ryang
Park, Jiyoung
Ryu, Hyung Won
Choi, Jang Hyun
Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title_full Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title_fullStr Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title_full_unstemmed Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title_short Broussonetia papyrifera Root Bark Extract Exhibits Anti-inflammatory Effects on Adipose Tissue and Improves Insulin Sensitivity Potentially Via AMPK Activation
title_sort broussonetia papyrifera root bark extract exhibits anti-inflammatory effects on adipose tissue and improves insulin sensitivity potentially via ampk activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146562/
https://www.ncbi.nlm.nih.gov/pubmed/32183397
http://dx.doi.org/10.3390/nu12030773
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