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Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice

Obesity is associated with metabolic disorders. Thus, obesity prevention and treatment are essential for health. Antrodia cinnamomea (AC) is a multifunctional medicinal fungus used for the treatment of various diseases and for preventing diet-induced obesity. Leptin deficiency causes over-eating and...

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Autores principales: Tsai, Yi-Ting, Ruan, Jhen-Wei, Chang, Cherng-Shyang, Ko, Mei-Lan, Chou, Hsiu-Chuan, Lin, Chi-Chien, Lin, Chiao-Mei, Huang, Chih-Ting, Wei, Yu-Shan, Liao, En-Chi, Chen, Hsin-Yi, Kao, Cheng-Yuan, Chan, Hong-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146579/
https://www.ncbi.nlm.nih.gov/pubmed/32164196
http://dx.doi.org/10.3390/nu12030726
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author Tsai, Yi-Ting
Ruan, Jhen-Wei
Chang, Cherng-Shyang
Ko, Mei-Lan
Chou, Hsiu-Chuan
Lin, Chi-Chien
Lin, Chiao-Mei
Huang, Chih-Ting
Wei, Yu-Shan
Liao, En-Chi
Chen, Hsin-Yi
Kao, Cheng-Yuan
Chan, Hong-Lin
author_facet Tsai, Yi-Ting
Ruan, Jhen-Wei
Chang, Cherng-Shyang
Ko, Mei-Lan
Chou, Hsiu-Chuan
Lin, Chi-Chien
Lin, Chiao-Mei
Huang, Chih-Ting
Wei, Yu-Shan
Liao, En-Chi
Chen, Hsin-Yi
Kao, Cheng-Yuan
Chan, Hong-Lin
author_sort Tsai, Yi-Ting
collection PubMed
description Obesity is associated with metabolic disorders. Thus, obesity prevention and treatment are essential for health. Antrodia cinnamomea (AC) is a multifunctional medicinal fungus used for the treatment of various diseases and for preventing diet-induced obesity. Leptin deficiency causes over-eating and spontaneous obesity. The concomitant metabolic symptoms are more severe than diet-induced obesity. Here, we used leptin-deficient (ob/ob) mice as an animal model for over-feeding to study the effect of AC on obesity. We fed C57BL/6 mice (WT, ob+/+) and ob/ob mice with AC for four weeks before performing qRT-PCR and immunoblot analysis to elaborate AC-modulated mechanisms. Further, we used Caco-2 cells as a human intestinal epithelial barrier model to examine the effect of AC on intestinal permeability. Our results suggested that AC reduces lipid deposits of the liver and epididymal white adipose tissue (EWAT) by promoting lipid metabolism and inhibiting lipogenesis-associated genes and proteins in ob/ob mice. Moreover, AC effectively repaired intestinal-barrier injury caused by leptin deficiency and enhanced intestinal barrier integrity in Caco-2 cells. Interestingly, AC significantly reduced body weight and EWAT with no compromise on food intake in ob/ob mice. Thus, AC effectively reduced obesity caused by leptin-deficiency and can potentially be used as a nutraceutical for treating obesity.
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spelling pubmed-71465792020-04-20 Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice Tsai, Yi-Ting Ruan, Jhen-Wei Chang, Cherng-Shyang Ko, Mei-Lan Chou, Hsiu-Chuan Lin, Chi-Chien Lin, Chiao-Mei Huang, Chih-Ting Wei, Yu-Shan Liao, En-Chi Chen, Hsin-Yi Kao, Cheng-Yuan Chan, Hong-Lin Nutrients Article Obesity is associated with metabolic disorders. Thus, obesity prevention and treatment are essential for health. Antrodia cinnamomea (AC) is a multifunctional medicinal fungus used for the treatment of various diseases and for preventing diet-induced obesity. Leptin deficiency causes over-eating and spontaneous obesity. The concomitant metabolic symptoms are more severe than diet-induced obesity. Here, we used leptin-deficient (ob/ob) mice as an animal model for over-feeding to study the effect of AC on obesity. We fed C57BL/6 mice (WT, ob+/+) and ob/ob mice with AC for four weeks before performing qRT-PCR and immunoblot analysis to elaborate AC-modulated mechanisms. Further, we used Caco-2 cells as a human intestinal epithelial barrier model to examine the effect of AC on intestinal permeability. Our results suggested that AC reduces lipid deposits of the liver and epididymal white adipose tissue (EWAT) by promoting lipid metabolism and inhibiting lipogenesis-associated genes and proteins in ob/ob mice. Moreover, AC effectively repaired intestinal-barrier injury caused by leptin deficiency and enhanced intestinal barrier integrity in Caco-2 cells. Interestingly, AC significantly reduced body weight and EWAT with no compromise on food intake in ob/ob mice. Thus, AC effectively reduced obesity caused by leptin-deficiency and can potentially be used as a nutraceutical for treating obesity. MDPI 2020-03-10 /pmc/articles/PMC7146579/ /pubmed/32164196 http://dx.doi.org/10.3390/nu12030726 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Yi-Ting
Ruan, Jhen-Wei
Chang, Cherng-Shyang
Ko, Mei-Lan
Chou, Hsiu-Chuan
Lin, Chi-Chien
Lin, Chiao-Mei
Huang, Chih-Ting
Wei, Yu-Shan
Liao, En-Chi
Chen, Hsin-Yi
Kao, Cheng-Yuan
Chan, Hong-Lin
Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title_full Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title_fullStr Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title_full_unstemmed Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title_short Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
title_sort antrodia cinnamomea confers obesity resistance and restores intestinal barrier integrity in leptin-deficient obese mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146579/
https://www.ncbi.nlm.nih.gov/pubmed/32164196
http://dx.doi.org/10.3390/nu12030726
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