The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples

The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast)...

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Autores principales: Pinto, Ana P., Vieira, Tales S., Marafon, Bruno B., Batitucci, Gabriela, Cabrera, Elisa M. B., da Rocha, Alisson L., Kohama, Eike B., Rodrigues, Kellen C. C., de Moura, Leandro P., Pauli, José R., Cintra, Dennys E., Ropelle, Eduardo R., de Freitas, Ellen C., da Silva, Adelino S. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146592/
https://www.ncbi.nlm.nih.gov/pubmed/32121154
http://dx.doi.org/10.3390/nu12030641
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author Pinto, Ana P.
Vieira, Tales S.
Marafon, Bruno B.
Batitucci, Gabriela
Cabrera, Elisa M. B.
da Rocha, Alisson L.
Kohama, Eike B.
Rodrigues, Kellen C. C.
de Moura, Leandro P.
Pauli, José R.
Cintra, Dennys E.
Ropelle, Eduardo R.
de Freitas, Ellen C.
da Silva, Adelino S. R.
author_facet Pinto, Ana P.
Vieira, Tales S.
Marafon, Bruno B.
Batitucci, Gabriela
Cabrera, Elisa M. B.
da Rocha, Alisson L.
Kohama, Eike B.
Rodrigues, Kellen C. C.
de Moura, Leandro P.
Pauli, José R.
Cintra, Dennys E.
Ropelle, Eduardo R.
de Freitas, Ellen C.
da Silva, Adelino S. R.
author_sort Pinto, Ana P.
collection PubMed
description The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes.
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spelling pubmed-71465922020-04-20 The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples Pinto, Ana P. Vieira, Tales S. Marafon, Bruno B. Batitucci, Gabriela Cabrera, Elisa M. B. da Rocha, Alisson L. Kohama, Eike B. Rodrigues, Kellen C. C. de Moura, Leandro P. Pauli, José R. Cintra, Dennys E. Ropelle, Eduardo R. de Freitas, Ellen C. da Silva, Adelino S. R. Nutrients Article The present study verified the responses of proteins related to the autophagy pathway after 10 h of fast with resistance exercise and protein ingestion in skeletal muscle and liver samples. The rats were distributed into five experimental groups: control (CT; sedentary and without gavage after fast), exercise immediately (EXE-imm; after fast, rats were submitted to the resistance protocol and received water by gavage immediately after exercise), exercise after 1 h (EXE-1h; after fast, rats were submitted to the resistance protocol and received water by gavage 1 h after exercise), exercise and supplementation immediately after exercise (EXE/Suppl-imm; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage immediately after exercise), exercise and supplementation 1 h after exercise (EXE/Suppl-1h; after fast, rats were submitted to the resistance protocol and received a mix of casein: whey protein 1:1 (w/w) by gavage 1 h after exercise). In summary, the current findings show that the combination of fasting, acute resistance exercise, and protein blend ingestion (immediately or 1 h after the exercise stimulus) increased the serum levels of leucine, insulin, and glucose, as well as the autophagy protein contents in skeletal muscle, but decreased other proteins related to the autophagic pathway in the liver. These results deserve further mechanistic investigations since athletes are combining fasting with physical exercise to enhance health and performance outcomes. MDPI 2020-02-28 /pmc/articles/PMC7146592/ /pubmed/32121154 http://dx.doi.org/10.3390/nu12030641 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinto, Ana P.
Vieira, Tales S.
Marafon, Bruno B.
Batitucci, Gabriela
Cabrera, Elisa M. B.
da Rocha, Alisson L.
Kohama, Eike B.
Rodrigues, Kellen C. C.
de Moura, Leandro P.
Pauli, José R.
Cintra, Dennys E.
Ropelle, Eduardo R.
de Freitas, Ellen C.
da Silva, Adelino S. R.
The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title_full The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title_fullStr The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title_full_unstemmed The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title_short The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples
title_sort combination of fasting, acute resistance exercise, and protein ingestion led to different responses of autophagy markers in gastrocnemius and liver samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146592/
https://www.ncbi.nlm.nih.gov/pubmed/32121154
http://dx.doi.org/10.3390/nu12030641
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