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Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor

Adiponectin, an adipokine produced and secreted by adipocytes, is involved in regulating the development and progression of insulin resistance, diabetes, and diabetic complications. Heat shock protein 60 (HSP60) is a molecular chaperone, most commonly presenting in mitochondria and participating in...

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Autores principales: Zhang, Deling, Liu, Hua, Zhang, Yemin, Li, Junfeng, Fu, Yalin, Zheng, Yuyang, Wu, Jie, Ma, Mingke, Wen, Zhongyuan, Wang, Changhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147001/
https://www.ncbi.nlm.nih.gov/pubmed/32272950
http://dx.doi.org/10.1186/s12964-020-00546-5
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author Zhang, Deling
Liu, Hua
Zhang, Yemin
Li, Junfeng
Fu, Yalin
Zheng, Yuyang
Wu, Jie
Ma, Mingke
Wen, Zhongyuan
Wang, Changhua
author_facet Zhang, Deling
Liu, Hua
Zhang, Yemin
Li, Junfeng
Fu, Yalin
Zheng, Yuyang
Wu, Jie
Ma, Mingke
Wen, Zhongyuan
Wang, Changhua
author_sort Zhang, Deling
collection PubMed
description Adiponectin, an adipokine produced and secreted by adipocytes, is involved in regulating the development and progression of insulin resistance, diabetes, and diabetic complications. Heat shock protein 60 (HSP60) is a molecular chaperone, most commonly presenting in mitochondria and participating in the maintenance of protein homeostasis. Accumulating studies have demonstrated that the elevated circulating HSP60 and the decreased intracellular HSP60 are closely associated with diabetic complications such as diabetic cardiomyopathy. However, the underlying mechanism remains poorly understood. In the present study, we reported that HSP60 interacted directly with adiponectin receptors. Its abundance was positively associated with adiponectin action. Furthermore, HSP60 depletion markedly mitigated the protective impacts of adiponectin on high glucose-induced oxidative stress and cell apoptosis in rat cardiac H9c2 cells. In addition, HSP60 knockdown significantly enhanced proteasome activity leading to the degradation of adiponectin receptor 1. Taken together, we showed for the first time that HSP60 interacted with adiponectin receptors and mediated adiponectin signaling through stabilizing adiponectin receptor. This in vitro study also provides an alternative explanation for mechanism by which adiponectin exerts its action.
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spelling pubmed-71470012020-04-18 Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor Zhang, Deling Liu, Hua Zhang, Yemin Li, Junfeng Fu, Yalin Zheng, Yuyang Wu, Jie Ma, Mingke Wen, Zhongyuan Wang, Changhua Cell Commun Signal Short Report Adiponectin, an adipokine produced and secreted by adipocytes, is involved in regulating the development and progression of insulin resistance, diabetes, and diabetic complications. Heat shock protein 60 (HSP60) is a molecular chaperone, most commonly presenting in mitochondria and participating in the maintenance of protein homeostasis. Accumulating studies have demonstrated that the elevated circulating HSP60 and the decreased intracellular HSP60 are closely associated with diabetic complications such as diabetic cardiomyopathy. However, the underlying mechanism remains poorly understood. In the present study, we reported that HSP60 interacted directly with adiponectin receptors. Its abundance was positively associated with adiponectin action. Furthermore, HSP60 depletion markedly mitigated the protective impacts of adiponectin on high glucose-induced oxidative stress and cell apoptosis in rat cardiac H9c2 cells. In addition, HSP60 knockdown significantly enhanced proteasome activity leading to the degradation of adiponectin receptor 1. Taken together, we showed for the first time that HSP60 interacted with adiponectin receptors and mediated adiponectin signaling through stabilizing adiponectin receptor. This in vitro study also provides an alternative explanation for mechanism by which adiponectin exerts its action. BioMed Central 2020-04-09 /pmc/articles/PMC7147001/ /pubmed/32272950 http://dx.doi.org/10.1186/s12964-020-00546-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Zhang, Deling
Liu, Hua
Zhang, Yemin
Li, Junfeng
Fu, Yalin
Zheng, Yuyang
Wu, Jie
Ma, Mingke
Wen, Zhongyuan
Wang, Changhua
Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title_full Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title_fullStr Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title_full_unstemmed Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title_short Heat shock protein 60 (HSP60) modulates adiponectin signaling by stabilizing adiponectin receptor
title_sort heat shock protein 60 (hsp60) modulates adiponectin signaling by stabilizing adiponectin receptor
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147001/
https://www.ncbi.nlm.nih.gov/pubmed/32272950
http://dx.doi.org/10.1186/s12964-020-00546-5
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