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A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1
BACKGROUND: Circular RNAs (circRNAs) are identified to play an important role in many human cancers, such as glioblastoma. However, the potential mechanisms underlying the relationship between circRNAs and glioblastoma pathogenesis are still elusive. This study is designed to investigate the role of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147020/ https://www.ncbi.nlm.nih.gov/pubmed/32308563 http://dx.doi.org/10.1186/s12935-020-01208-9 |
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author | Zhu, Fei Cheng, Cheng qin, Hong Wang, Hongsheng Yu, Hailong |
author_facet | Zhu, Fei Cheng, Cheng qin, Hong Wang, Hongsheng Yu, Hailong |
author_sort | Zhu, Fei |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) are identified to play an important role in many human cancers, such as glioblastoma. However, the potential mechanisms underlying the relationship between circRNAs and glioblastoma pathogenesis are still elusive. This study is designed to investigate the role of circRNAs in glioblastoma progression. METHODS: The present study is designed to investigate the mechanism by which circRNAs involves in glioblastoma pathogenesis. By using circRNAs microarray, we detected the dysregulated circRNAs and identified an up-regulated circRNA, circENTPD7 in glioblastoma tissues. Cell proliferation was measured using a CCK-8 assay. Cell clone formation ability was assessed with a clone formation test. We used the bioinformatics website to predict circRNA–miRNA and miRNA–mRNA interactions. CircRNA–miRNA interaction was confirmed by dual-luciferase reporter assays and RNA–RNA pulldown assay. RESULTS: circENTPD7 (hsa_circ_0019421) was upregulated in glioblastoma tissues. Kaplan–Meier survival analysis indicated that glioblastoma patients had a poor overall survival when circENTPD7 expression levels were high. Knockdown of circENTPD7 inhibited the motility and proliferation of glioblastoma cells. Moreover, we demonstrated that circENTPD7 acted as a sponge of miR-101-3p to regulate the expression of ROS1 further promoted the proliferation and motility of glioblastoma cells. CONCLUSIONS: Taken together, these findings indicate that circRNA circENTPD7 promotes glioblastoma cell proliferation and motility by regulating miR-101-3p/ROS1. |
format | Online Article Text |
id | pubmed-7147020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71470202020-04-18 A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 Zhu, Fei Cheng, Cheng qin, Hong Wang, Hongsheng Yu, Hailong Cancer Cell Int Primary Research BACKGROUND: Circular RNAs (circRNAs) are identified to play an important role in many human cancers, such as glioblastoma. However, the potential mechanisms underlying the relationship between circRNAs and glioblastoma pathogenesis are still elusive. This study is designed to investigate the role of circRNAs in glioblastoma progression. METHODS: The present study is designed to investigate the mechanism by which circRNAs involves in glioblastoma pathogenesis. By using circRNAs microarray, we detected the dysregulated circRNAs and identified an up-regulated circRNA, circENTPD7 in glioblastoma tissues. Cell proliferation was measured using a CCK-8 assay. Cell clone formation ability was assessed with a clone formation test. We used the bioinformatics website to predict circRNA–miRNA and miRNA–mRNA interactions. CircRNA–miRNA interaction was confirmed by dual-luciferase reporter assays and RNA–RNA pulldown assay. RESULTS: circENTPD7 (hsa_circ_0019421) was upregulated in glioblastoma tissues. Kaplan–Meier survival analysis indicated that glioblastoma patients had a poor overall survival when circENTPD7 expression levels were high. Knockdown of circENTPD7 inhibited the motility and proliferation of glioblastoma cells. Moreover, we demonstrated that circENTPD7 acted as a sponge of miR-101-3p to regulate the expression of ROS1 further promoted the proliferation and motility of glioblastoma cells. CONCLUSIONS: Taken together, these findings indicate that circRNA circENTPD7 promotes glioblastoma cell proliferation and motility by regulating miR-101-3p/ROS1. BioMed Central 2020-04-10 /pmc/articles/PMC7147020/ /pubmed/32308563 http://dx.doi.org/10.1186/s12935-020-01208-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Zhu, Fei Cheng, Cheng qin, Hong Wang, Hongsheng Yu, Hailong A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title | A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title_full | A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title_fullStr | A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title_full_unstemmed | A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title_short | A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1 |
title_sort | novel circular rna circentpd7 contributes to glioblastoma progression by targeting ros1 |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147020/ https://www.ncbi.nlm.nih.gov/pubmed/32308563 http://dx.doi.org/10.1186/s12935-020-01208-9 |
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