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Antiviral activity of Jinchai capsule against influenza virus
OBJECTIVE: To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine. METHODS: Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorpt...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Editorial Board of Journal of Traditional Chinese Medicine. Published by Elsevier (Singapore) Pte Ltd.
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147227/ https://www.ncbi.nlm.nih.gov/pubmed/23789217 http://dx.doi.org/10.1016/S0254-6272(13)60125-9 |
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author | Zhong, Juying Cui, Xiaolan Shi, Yujing Gao, Yingjie Cao, Hongxin |
author_facet | Zhong, Juying Cui, Xiaolan Shi, Yujing Gao, Yingjie Cao, Hongxin |
author_sort | Zhong, Juying |
collection | PubMed |
description | OBJECTIVE: To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine. METHODS: Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin (HA) protein, intracellular pH, and influenza virus protein acid (PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction. RESULTS: Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells. Jinchai also significantly decreased intracellular pH in infected cells. CONCLUSIONS: Jinchai had strong anti-influenza activity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replication of the virus. |
format | Online Article Text |
id | pubmed-7147227 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Editorial Board of Journal of Traditional Chinese Medicine. Published by Elsevier (Singapore) Pte Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71472272020-04-10 Antiviral activity of Jinchai capsule against influenza virus Zhong, Juying Cui, Xiaolan Shi, Yujing Gao, Yingjie Cao, Hongxin Journal of Traditional Chinese Medicine Article OBJECTIVE: To evaluate the effect on influenza virus of Jinchai, a capsule made of Traditional Chinese Medicine. METHODS: Madin-darby canine kidney (MDCK) cells were infected with the FM1 strain of influenza virus A (subtype H1N1) in vitro. They were used to explore how Jinchai affected cell adsorption, cell membrane fusion, transcription and replication of the influenza virus. Hemagglutinin (HA) protein, intracellular pH, and influenza virus protein acid (PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction. RESULTS: Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells. Jinchai also significantly decreased intracellular pH in infected cells. CONCLUSIONS: Jinchai had strong anti-influenza activity against the influenza virus. It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage, and inhibited the transcription and replication of the virus. The Editorial Board of Journal of Traditional Chinese Medicine. Published by Elsevier (Singapore) Pte Ltd. 2013-04 2013-09-13 /pmc/articles/PMC7147227/ /pubmed/23789217 http://dx.doi.org/10.1016/S0254-6272(13)60125-9 Text en © 2013 The Editorial Board of Journal of Traditional Chinese Medicine Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Zhong, Juying Cui, Xiaolan Shi, Yujing Gao, Yingjie Cao, Hongxin Antiviral activity of Jinchai capsule against influenza virus |
title | Antiviral activity of Jinchai capsule against influenza virus |
title_full | Antiviral activity of Jinchai capsule against influenza virus |
title_fullStr | Antiviral activity of Jinchai capsule against influenza virus |
title_full_unstemmed | Antiviral activity of Jinchai capsule against influenza virus |
title_short | Antiviral activity of Jinchai capsule against influenza virus |
title_sort | antiviral activity of jinchai capsule against influenza virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147227/ https://www.ncbi.nlm.nih.gov/pubmed/23789217 http://dx.doi.org/10.1016/S0254-6272(13)60125-9 |
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