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Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts

The purpose of this article was to explore the effects of gold nanoparticles (GNPs) and silver nanoparticles (SNPs) with different cytotoxicities on human dermal fibroblasts (HDFs) at the metabolic level. First, ∼20 nm of GNPs and SNPs were prepared, and their effects on the proliferation of HDFs we...

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Autores principales: Huang, Yan, Lü, Xiaoying, Chen, Rong, Chen, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147366/
https://www.ncbi.nlm.nih.gov/pubmed/32296541
http://dx.doi.org/10.1093/rb/rbz051
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author Huang, Yan
Lü, Xiaoying
Chen, Rong
Chen, Ye
author_facet Huang, Yan
Lü, Xiaoying
Chen, Rong
Chen, Ye
author_sort Huang, Yan
collection PubMed
description The purpose of this article was to explore the effects of gold nanoparticles (GNPs) and silver nanoparticles (SNPs) with different cytotoxicities on human dermal fibroblasts (HDFs) at the metabolic level. First, ∼20 nm of GNPs and SNPs were prepared, and their effects on the proliferation of HDFs were evaluated. Then, a metabolomics technique was used to analyse the effects of GNPs and SNPs on the expression profiles of metabolites in HDFs after 4, 8 and 24 h of treatment. Furthermore, the key metabolites and key metabolic pathways involved in the interaction of GNPs and SNPs with HDFs were identified through expression pattern analysis and metabolic pathway analysis of differentially expressed metabolites and were finally verified by experiments. The results of the cytotoxicity experiments showed that there was no cytotoxicity after the treatment of GNPs for 72 h, while the cytotoxicity of the SNPs reached grade 1 after 72 h. By using metabolomics analysis, 29, 30 and 27 metabolites were shown to be differentially expressed in HDFs after GNP treatment, while SNPs induced the differential expression of 13, 33 and 22 metabolites after 4, 8 and 24 h of treatment, respectively. Six and four candidate key metabolites in the GNP and SNP groups were identified by expression pattern analysis and metabolic pathway analysis, respectively. The key metabolic pathways in the GNP and SNP groups were identified as the glutathione metabolic pathway (the key metabolite of which was glutathione) and the citrate cycle pathway (the key metabolite of which was malic acid). Based on the experiments used to verify the key metabolites and key metabolic pathways, it was found that the increase in glutathione after GNP treatment might trigger an oxidative stress protection mechanism and thus avoid cytotoxicity. After exposure to SNPs, the citric acid content was increased, mainly through the citrate cycle pathway, thereby inhibiting the synthesis of malic acid to affect the formation of ATP and finally leading to cytotoxicity.
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spelling pubmed-71473662020-04-15 Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts Huang, Yan Lü, Xiaoying Chen, Rong Chen, Ye Regen Biomater Research Articles The purpose of this article was to explore the effects of gold nanoparticles (GNPs) and silver nanoparticles (SNPs) with different cytotoxicities on human dermal fibroblasts (HDFs) at the metabolic level. First, ∼20 nm of GNPs and SNPs were prepared, and their effects on the proliferation of HDFs were evaluated. Then, a metabolomics technique was used to analyse the effects of GNPs and SNPs on the expression profiles of metabolites in HDFs after 4, 8 and 24 h of treatment. Furthermore, the key metabolites and key metabolic pathways involved in the interaction of GNPs and SNPs with HDFs were identified through expression pattern analysis and metabolic pathway analysis of differentially expressed metabolites and were finally verified by experiments. The results of the cytotoxicity experiments showed that there was no cytotoxicity after the treatment of GNPs for 72 h, while the cytotoxicity of the SNPs reached grade 1 after 72 h. By using metabolomics analysis, 29, 30 and 27 metabolites were shown to be differentially expressed in HDFs after GNP treatment, while SNPs induced the differential expression of 13, 33 and 22 metabolites after 4, 8 and 24 h of treatment, respectively. Six and four candidate key metabolites in the GNP and SNP groups were identified by expression pattern analysis and metabolic pathway analysis, respectively. The key metabolic pathways in the GNP and SNP groups were identified as the glutathione metabolic pathway (the key metabolite of which was glutathione) and the citrate cycle pathway (the key metabolite of which was malic acid). Based on the experiments used to verify the key metabolites and key metabolic pathways, it was found that the increase in glutathione after GNP treatment might trigger an oxidative stress protection mechanism and thus avoid cytotoxicity. After exposure to SNPs, the citric acid content was increased, mainly through the citrate cycle pathway, thereby inhibiting the synthesis of malic acid to affect the formation of ATP and finally leading to cytotoxicity. Oxford University Press 2020-03 2020-01-25 /pmc/articles/PMC7147366/ /pubmed/32296541 http://dx.doi.org/10.1093/rb/rbz051 Text en © The Author(s) 2020. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Huang, Yan
Lü, Xiaoying
Chen, Rong
Chen, Ye
Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title_full Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title_fullStr Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title_full_unstemmed Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title_short Comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
title_sort comparative study of the effects of gold and silver nanoparticles on the metabolism of human dermal fibroblasts
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147366/
https://www.ncbi.nlm.nih.gov/pubmed/32296541
http://dx.doi.org/10.1093/rb/rbz051
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