Cargando…

Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland

BACKGROUND: Human adenovirus (HAdV) is an important pathogen seen in clinical practice. Long-term studies may help better understand epidemiological trends and changes in circulating genotypes over time. PURPOSE: Using a large biobank of samples from hospitalized, adenovirus-positive patients over a...

Descripción completa

Detalles Bibliográficos
Autores principales: Akello, Joyce Odeke, Kamgang, Richard, Barbani, Maria Teresa, Suter-Riniker, Franziska, Leib, Stephen L, Ramette, Alban
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147615/
https://www.ncbi.nlm.nih.gov/pubmed/32308491
http://dx.doi.org/10.2147/CLEP.S246352
_version_ 1783520448513835008
author Akello, Joyce Odeke
Kamgang, Richard
Barbani, Maria Teresa
Suter-Riniker, Franziska
Leib, Stephen L
Ramette, Alban
author_facet Akello, Joyce Odeke
Kamgang, Richard
Barbani, Maria Teresa
Suter-Riniker, Franziska
Leib, Stephen L
Ramette, Alban
author_sort Akello, Joyce Odeke
collection PubMed
description BACKGROUND: Human adenovirus (HAdV) is an important pathogen seen in clinical practice. Long-term studies may help better understand epidemiological trends and changes in circulating genotypes over time. PURPOSE: Using a large biobank of samples from hospitalized, adenovirus-positive patients over a 20-year period, we aimed to analyze long-term epidemiological trends and genotypic relatedness among circulating HAdV strains. METHODS: Based on samples from hospitalized patients confirmed to be HAdV positive in Bern, Switzerland, from 1998 to 2017, and on their associated demographic and clinical data, we identified epidemiological trends and risk factors associated with HAdV infection. HAdV genotyping was performed by PCR amplification and sequencing of the hypervariable hexon gene. The obtained sequences were phylogenetically compared with sequences from international HAdV strains. RESULTS: HAdV was identified in 1302 samples tested. Cases of HAdV infection were reported throughout the years with no clear seasonality. Upper respiratory tract samples, conjunctivitis swabs, and stool had the highest positivity rate (56.2%, 18.7%, and 14.2% of the cases, respectively). HAdV infection was highest among children ≤4 years old. Increased number of HAdV cases were observed in years 2009 (n = 110) and 2010 (n =112). HAdV8 was the predominant genotype among patients older than 20 years, and was mostly associated with ophthalmic infection. Predominant genotypes among children ≤4 years old were HAdV1, HAdV2, and HAdV3, which were mostly associated with respiratory tract infections. Recurring peaks of increased HAdV cases were evidenced every 4 years among children ≤4 years old. CONCLUSION: Our study gives novel insights on long-term epidemiological trends and phylogenetic relatedness among circulating HAdV strains in Switzerland, country in which little data on HAdV prevalence and diversity was so far available.
format Online
Article
Text
id pubmed-7147615
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-71476152020-04-17 Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland Akello, Joyce Odeke Kamgang, Richard Barbani, Maria Teresa Suter-Riniker, Franziska Leib, Stephen L Ramette, Alban Clin Epidemiol Original Research BACKGROUND: Human adenovirus (HAdV) is an important pathogen seen in clinical practice. Long-term studies may help better understand epidemiological trends and changes in circulating genotypes over time. PURPOSE: Using a large biobank of samples from hospitalized, adenovirus-positive patients over a 20-year period, we aimed to analyze long-term epidemiological trends and genotypic relatedness among circulating HAdV strains. METHODS: Based on samples from hospitalized patients confirmed to be HAdV positive in Bern, Switzerland, from 1998 to 2017, and on their associated demographic and clinical data, we identified epidemiological trends and risk factors associated with HAdV infection. HAdV genotyping was performed by PCR amplification and sequencing of the hypervariable hexon gene. The obtained sequences were phylogenetically compared with sequences from international HAdV strains. RESULTS: HAdV was identified in 1302 samples tested. Cases of HAdV infection were reported throughout the years with no clear seasonality. Upper respiratory tract samples, conjunctivitis swabs, and stool had the highest positivity rate (56.2%, 18.7%, and 14.2% of the cases, respectively). HAdV infection was highest among children ≤4 years old. Increased number of HAdV cases were observed in years 2009 (n = 110) and 2010 (n =112). HAdV8 was the predominant genotype among patients older than 20 years, and was mostly associated with ophthalmic infection. Predominant genotypes among children ≤4 years old were HAdV1, HAdV2, and HAdV3, which were mostly associated with respiratory tract infections. Recurring peaks of increased HAdV cases were evidenced every 4 years among children ≤4 years old. CONCLUSION: Our study gives novel insights on long-term epidemiological trends and phylogenetic relatedness among circulating HAdV strains in Switzerland, country in which little data on HAdV prevalence and diversity was so far available. Dove 2020-04-05 /pmc/articles/PMC7147615/ /pubmed/32308491 http://dx.doi.org/10.2147/CLEP.S246352 Text en © 2020 Akello et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Akello, Joyce Odeke
Kamgang, Richard
Barbani, Maria Teresa
Suter-Riniker, Franziska
Leib, Stephen L
Ramette, Alban
Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title_full Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title_fullStr Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title_full_unstemmed Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title_short Epidemiology of Human Adenoviruses: A 20-Year Retrospective Observational Study in Hospitalized Patients in Bern, Switzerland
title_sort epidemiology of human adenoviruses: a 20-year retrospective observational study in hospitalized patients in bern, switzerland
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7147615/
https://www.ncbi.nlm.nih.gov/pubmed/32308491
http://dx.doi.org/10.2147/CLEP.S246352
work_keys_str_mv AT akellojoyceodeke epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland
AT kamgangrichard epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland
AT barbanimariateresa epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland
AT suterrinikerfranziska epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland
AT leibstephenl epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland
AT ramettealban epidemiologyofhumanadenovirusesa20yearretrospectiveobservationalstudyinhospitalizedpatientsinbernswitzerland