Cargando…

Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota

Female genital tract (FGT) inflammation increases HIV infection susceptibility. Non-optimal cervicovaginal microbiota, characterized by depletion of Lactobacillus species and increased bacterial diversity, is associated with increased FGT cytokine production. Lactobacillus species may protect agains...

Descripción completa

Detalles Bibliográficos
Autores principales: Manhanzva, Monalisa T., Abrahams, Andrea G., Gamieldien, Hoyam, Froissart, Remy, Jaspan, Heather, Jaumdally, Shameem Z., Barnabas, Shaun L., Dabee, Smritee, Bekker, Linda G., Gray, Glenda, Passmore, Jo-Ann S., Masson, Lindi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148372/
https://www.ncbi.nlm.nih.gov/pubmed/32277092
http://dx.doi.org/10.1038/s41598-020-62184-8
_version_ 1783520582378192896
author Manhanzva, Monalisa T.
Abrahams, Andrea G.
Gamieldien, Hoyam
Froissart, Remy
Jaspan, Heather
Jaumdally, Shameem Z.
Barnabas, Shaun L.
Dabee, Smritee
Bekker, Linda G.
Gray, Glenda
Passmore, Jo-Ann S.
Masson, Lindi
author_facet Manhanzva, Monalisa T.
Abrahams, Andrea G.
Gamieldien, Hoyam
Froissart, Remy
Jaspan, Heather
Jaumdally, Shameem Z.
Barnabas, Shaun L.
Dabee, Smritee
Bekker, Linda G.
Gray, Glenda
Passmore, Jo-Ann S.
Masson, Lindi
author_sort Manhanzva, Monalisa T.
collection PubMed
description Female genital tract (FGT) inflammation increases HIV infection susceptibility. Non-optimal cervicovaginal microbiota, characterized by depletion of Lactobacillus species and increased bacterial diversity, is associated with increased FGT cytokine production. Lactobacillus species may protect against HIV partly by reducing FGT inflammation. We isolated 80 lactobacilli from South African women with non-optimal (Nugent 4–10; n = 18) and optimal microbiota (Nugent 0–3; n = 14). Cytokine production by vaginal epithelial cells in response to lactobacilli in the presence and absence of Gardnerella vaginalis was measured using Luminex. Adhesion to vaginal epithelial cells, pH, D/L-lactate production and lactate dehydrogenase relative abundance were assessed. Lactobacilli from women with non-optimal produced less lactic acid and induced greater inflammatory cytokine production than those from women with optimal microbiota, with IL-6, IL-8, IL-1α, IL-1β and MIP-1α/β production significantly elevated. Overall, lactobacilli suppressed IL-6 (adjusted p < 0.001) and IL-8 (adjusted p = 0.0170) responses to G. vaginalis. Cytokine responses to the lactobacilli were inversely associated with lactobacilli adhesion to epithelial cells and D-lactate dehydrogenase relative abundance. Thus, while cervicovaginal lactobacilli reduced the production of the majority of inflammatory cytokines in response to G. vaginalis, isolates from women with non-optimal microbiota were more inflammatory and produced less lactic acid than isolates from women with optimal microbiota.
format Online
Article
Text
id pubmed-7148372
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-71483722020-04-15 Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota Manhanzva, Monalisa T. Abrahams, Andrea G. Gamieldien, Hoyam Froissart, Remy Jaspan, Heather Jaumdally, Shameem Z. Barnabas, Shaun L. Dabee, Smritee Bekker, Linda G. Gray, Glenda Passmore, Jo-Ann S. Masson, Lindi Sci Rep Article Female genital tract (FGT) inflammation increases HIV infection susceptibility. Non-optimal cervicovaginal microbiota, characterized by depletion of Lactobacillus species and increased bacterial diversity, is associated with increased FGT cytokine production. Lactobacillus species may protect against HIV partly by reducing FGT inflammation. We isolated 80 lactobacilli from South African women with non-optimal (Nugent 4–10; n = 18) and optimal microbiota (Nugent 0–3; n = 14). Cytokine production by vaginal epithelial cells in response to lactobacilli in the presence and absence of Gardnerella vaginalis was measured using Luminex. Adhesion to vaginal epithelial cells, pH, D/L-lactate production and lactate dehydrogenase relative abundance were assessed. Lactobacilli from women with non-optimal produced less lactic acid and induced greater inflammatory cytokine production than those from women with optimal microbiota, with IL-6, IL-8, IL-1α, IL-1β and MIP-1α/β production significantly elevated. Overall, lactobacilli suppressed IL-6 (adjusted p < 0.001) and IL-8 (adjusted p = 0.0170) responses to G. vaginalis. Cytokine responses to the lactobacilli were inversely associated with lactobacilli adhesion to epithelial cells and D-lactate dehydrogenase relative abundance. Thus, while cervicovaginal lactobacilli reduced the production of the majority of inflammatory cytokines in response to G. vaginalis, isolates from women with non-optimal microbiota were more inflammatory and produced less lactic acid than isolates from women with optimal microbiota. Nature Publishing Group UK 2020-04-10 /pmc/articles/PMC7148372/ /pubmed/32277092 http://dx.doi.org/10.1038/s41598-020-62184-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Manhanzva, Monalisa T.
Abrahams, Andrea G.
Gamieldien, Hoyam
Froissart, Remy
Jaspan, Heather
Jaumdally, Shameem Z.
Barnabas, Shaun L.
Dabee, Smritee
Bekker, Linda G.
Gray, Glenda
Passmore, Jo-Ann S.
Masson, Lindi
Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title_full Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title_fullStr Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title_full_unstemmed Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title_short Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota
title_sort inflammatory and antimicrobial properties differ between vaginal lactobacillus isolates from south african women with non-optimal versus optimal microbiota
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148372/
https://www.ncbi.nlm.nih.gov/pubmed/32277092
http://dx.doi.org/10.1038/s41598-020-62184-8
work_keys_str_mv AT manhanzvamonalisat inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT abrahamsandreag inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT gamieldienhoyam inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT froissartremy inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT jaspanheather inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT jaumdallyshameemz inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT barnabasshaunl inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT dabeesmritee inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT bekkerlindag inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT grayglenda inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT passmorejoanns inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota
AT massonlindi inflammatoryandantimicrobialpropertiesdifferbetweenvaginallactobacillusisolatesfromsouthafricanwomenwithnonoptimalversusoptimalmicrobiota