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New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities

Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, Ijuhya vitellina, an antagonistic fungus previously isolated from the plant parasitic cyst nematode Heterodera filipjevi, was selected to carry out an in-dept...

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Autores principales: Moussa, Ashaimaa Y., Lambert, Christopher, Stradal, Theresia E.B., Ashrafi, Samad, Maier, Wolfgang, Stadler, Marc, Helaly, Soleiman E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148537/
https://www.ncbi.nlm.nih.gov/pubmed/32235703
http://dx.doi.org/10.3390/antibiotics9030132
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author Moussa, Ashaimaa Y.
Lambert, Christopher
Stradal, Theresia E.B.
Ashrafi, Samad
Maier, Wolfgang
Stadler, Marc
Helaly, Soleiman E.
author_facet Moussa, Ashaimaa Y.
Lambert, Christopher
Stradal, Theresia E.B.
Ashrafi, Samad
Maier, Wolfgang
Stadler, Marc
Helaly, Soleiman E.
author_sort Moussa, Ashaimaa Y.
collection PubMed
description Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, Ijuhya vitellina, an antagonistic fungus previously isolated from the plant parasitic cyst nematode Heterodera filipjevi, was selected to carry out an in-depth metabolomic study for its active metabolites. Herein, three new nonapeptide peptaibols with leucinostatin based sequences were isolated and identified by 1, 2D NMR, and HR-ESI-MS-MS. The absolute configuration was assigned based on Marfay’s analysis and Mosher ester formation. The new leucinostatins manifested moderate nematicidal effect against the plant pathogenic nematode Pratylenchus penetrans with LD(90) values ranging from 5 to 7 µg/mL. Furthermore, a cyclodepsipeptide, named arthrichitin D, with five amino acid residues attached to a 3-hydroxy-2,4-dimethylhexadeca-4,6-dienoic fatty acid chain was discovered and showed weak nematicidal effect against Caenorhabditis elegans. Chaetoglobosin B and its 19-O-acetyl derivative were also obtained as minor metabolites, and the activity of chaetoglobosin B on the actin cytoskeleton of mammalian cells was assessed.
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spelling pubmed-71485372020-04-20 New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities Moussa, Ashaimaa Y. Lambert, Christopher Stradal, Theresia E.B. Ashrafi, Samad Maier, Wolfgang Stadler, Marc Helaly, Soleiman E. Antibiotics (Basel) Article Fungal associations with nematodes have attracted scientific attention because of the need to develop new biocontrol agents. In this context, Ijuhya vitellina, an antagonistic fungus previously isolated from the plant parasitic cyst nematode Heterodera filipjevi, was selected to carry out an in-depth metabolomic study for its active metabolites. Herein, three new nonapeptide peptaibols with leucinostatin based sequences were isolated and identified by 1, 2D NMR, and HR-ESI-MS-MS. The absolute configuration was assigned based on Marfay’s analysis and Mosher ester formation. The new leucinostatins manifested moderate nematicidal effect against the plant pathogenic nematode Pratylenchus penetrans with LD(90) values ranging from 5 to 7 µg/mL. Furthermore, a cyclodepsipeptide, named arthrichitin D, with five amino acid residues attached to a 3-hydroxy-2,4-dimethylhexadeca-4,6-dienoic fatty acid chain was discovered and showed weak nematicidal effect against Caenorhabditis elegans. Chaetoglobosin B and its 19-O-acetyl derivative were also obtained as minor metabolites, and the activity of chaetoglobosin B on the actin cytoskeleton of mammalian cells was assessed. MDPI 2020-03-22 /pmc/articles/PMC7148537/ /pubmed/32235703 http://dx.doi.org/10.3390/antibiotics9030132 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moussa, Ashaimaa Y.
Lambert, Christopher
Stradal, Theresia E.B.
Ashrafi, Samad
Maier, Wolfgang
Stadler, Marc
Helaly, Soleiman E.
New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title_full New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title_fullStr New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title_full_unstemmed New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title_short New Peptaibiotics and a Cyclodepsipeptide from Ijuhya vitellina: Isolation, Identification, Cytotoxic and Nematicidal Activities
title_sort new peptaibiotics and a cyclodepsipeptide from ijuhya vitellina: isolation, identification, cytotoxic and nematicidal activities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148537/
https://www.ncbi.nlm.nih.gov/pubmed/32235703
http://dx.doi.org/10.3390/antibiotics9030132
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