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Switching fatty acid metabolism by an RNA-controlled feed forward loop
Hfq (host factor for phage Q beta) is key for posttranscriptional gene regulation in many bacteria. Hfq’s function is to stabilize sRNAs and to facilitate base-pairing with trans-encoded target mRNAs. Loss of Hfq typically results in pleiotropic phenotypes, and, in the major human pathogen Vibrio ch...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148568/ https://www.ncbi.nlm.nih.gov/pubmed/32193348 http://dx.doi.org/10.1073/pnas.1920753117 |
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author | Huber, Michaela Fröhlich, Kathrin S. Radmer, Jessica Papenfort, Kai |
author_facet | Huber, Michaela Fröhlich, Kathrin S. Radmer, Jessica Papenfort, Kai |
author_sort | Huber, Michaela |
collection | PubMed |
description | Hfq (host factor for phage Q beta) is key for posttranscriptional gene regulation in many bacteria. Hfq’s function is to stabilize sRNAs and to facilitate base-pairing with trans-encoded target mRNAs. Loss of Hfq typically results in pleiotropic phenotypes, and, in the major human pathogen Vibrio cholerae, Hfq inactivation has been linked to reduced virulence, failure to produce biofilms, and impaired intercellular communication. However, the RNA ligands of Hfq in V. cholerae are currently unknown. Here, we used RIP-seq (RNA immunoprecipitation followed by high-throughput sequencing) analysis to identify Hfq-bound RNAs in V. cholerae. Our work revealed 603 coding and 85 noncoding transcripts associated with Hfq, including 44 sRNAs originating from the 3′ end of mRNAs. Detailed investigation of one of these latter transcripts, named FarS (fatty acid regulated sRNA), showed that this sRNA is produced by RNase E-mediated maturation of the fabB 3′UTR, and, together with Hfq, inhibits the expression of two paralogous fadE mRNAs. The fabB and fadE genes are antagonistically regulated by the major fatty acid transcription factor, FadR, and we show that, together, FadR, FarS, and FadE constitute a mixed feed-forward loop regulating the transition between fatty acid biosynthesis and degradation in V. cholerae. Our results provide the molecular basis for studies on Hfq in V. cholerae and highlight the importance of a previously unrecognized sRNA for fatty acid metabolism in this major human pathogen. |
format | Online Article Text |
id | pubmed-7148568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-71485682020-04-15 Switching fatty acid metabolism by an RNA-controlled feed forward loop Huber, Michaela Fröhlich, Kathrin S. Radmer, Jessica Papenfort, Kai Proc Natl Acad Sci U S A Biological Sciences Hfq (host factor for phage Q beta) is key for posttranscriptional gene regulation in many bacteria. Hfq’s function is to stabilize sRNAs and to facilitate base-pairing with trans-encoded target mRNAs. Loss of Hfq typically results in pleiotropic phenotypes, and, in the major human pathogen Vibrio cholerae, Hfq inactivation has been linked to reduced virulence, failure to produce biofilms, and impaired intercellular communication. However, the RNA ligands of Hfq in V. cholerae are currently unknown. Here, we used RIP-seq (RNA immunoprecipitation followed by high-throughput sequencing) analysis to identify Hfq-bound RNAs in V. cholerae. Our work revealed 603 coding and 85 noncoding transcripts associated with Hfq, including 44 sRNAs originating from the 3′ end of mRNAs. Detailed investigation of one of these latter transcripts, named FarS (fatty acid regulated sRNA), showed that this sRNA is produced by RNase E-mediated maturation of the fabB 3′UTR, and, together with Hfq, inhibits the expression of two paralogous fadE mRNAs. The fabB and fadE genes are antagonistically regulated by the major fatty acid transcription factor, FadR, and we show that, together, FadR, FarS, and FadE constitute a mixed feed-forward loop regulating the transition between fatty acid biosynthesis and degradation in V. cholerae. Our results provide the molecular basis for studies on Hfq in V. cholerae and highlight the importance of a previously unrecognized sRNA for fatty acid metabolism in this major human pathogen. National Academy of Sciences 2020-04-07 2020-03-19 /pmc/articles/PMC7148568/ /pubmed/32193348 http://dx.doi.org/10.1073/pnas.1920753117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Huber, Michaela Fröhlich, Kathrin S. Radmer, Jessica Papenfort, Kai Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title | Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title_full | Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title_fullStr | Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title_full_unstemmed | Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title_short | Switching fatty acid metabolism by an RNA-controlled feed forward loop |
title_sort | switching fatty acid metabolism by an rna-controlled feed forward loop |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148568/ https://www.ncbi.nlm.nih.gov/pubmed/32193348 http://dx.doi.org/10.1073/pnas.1920753117 |
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