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Diagnosis and Methods
A number of methodologies are currently available for viral diagnosis and basic research, ranging from immunological methods, such as ELISA, to molecular methods, such as PCR-based diagnosis. Other immunological methods include immunofluorescence assay and immunoblot. In addition, some classical met...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148742/ http://dx.doi.org/10.1016/B978-0-12-800838-6.00004-7 |
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author | Ryu, Wang-Shick |
author_facet | Ryu, Wang-Shick |
author_sort | Ryu, Wang-Shick |
collection | PubMed |
description | A number of methodologies are currently available for viral diagnosis and basic research, ranging from immunological methods, such as ELISA, to molecular methods, such as PCR-based diagnosis. Other immunological methods include immunofluorescence assay and immunoblot. In addition, some classical methods, such as plaque assay and hemagglutination, are still utilized in laboratories. Tissue culture is conveniently used for virus cultivation and propagation. Experimental animals are used for studies involving viral pathogenesis. Density gradient centrifugation is used for the purification of virus particles. Finally, molecular methods are used for the genetic analysis (ie, mutation) of viral gene function by using the replicon. |
format | Online Article Text |
id | pubmed-7148742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71487422020-04-13 Diagnosis and Methods Ryu, Wang-Shick Molecular Virology of Human Pathogenic Viruses Article A number of methodologies are currently available for viral diagnosis and basic research, ranging from immunological methods, such as ELISA, to molecular methods, such as PCR-based diagnosis. Other immunological methods include immunofluorescence assay and immunoblot. In addition, some classical methods, such as plaque assay and hemagglutination, are still utilized in laboratories. Tissue culture is conveniently used for virus cultivation and propagation. Experimental animals are used for studies involving viral pathogenesis. Density gradient centrifugation is used for the purification of virus particles. Finally, molecular methods are used for the genetic analysis (ie, mutation) of viral gene function by using the replicon. 2017 2016-05-06 /pmc/articles/PMC7148742/ http://dx.doi.org/10.1016/B978-0-12-800838-6.00004-7 Text en Copyright © 2017 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Ryu, Wang-Shick Diagnosis and Methods |
title | Diagnosis and Methods |
title_full | Diagnosis and Methods |
title_fullStr | Diagnosis and Methods |
title_full_unstemmed | Diagnosis and Methods |
title_short | Diagnosis and Methods |
title_sort | diagnosis and methods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148742/ http://dx.doi.org/10.1016/B978-0-12-800838-6.00004-7 |
work_keys_str_mv | AT ryuwangshick diagnosisandmethods |