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Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study

BACKGROUND: Type 1 diabetes is a chronic disease including hyperglycemia and accelerated atherosclerosis, with high risk of micro- and macrovascular complications. Circulating microvesicles (cMVs) are procoagulant cell fragments shed during activation/apoptosis and discussed to be markers of vascula...

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Autores principales: Bratseth, Vibeke, Margeirsdottir, Hanna D., Chiva-Blanch, Gemma, Heier, Martin, Solheim, Svein, Arnesen, Harald, Dahl-Jørgensen, Knut, Seljeflot, Ingebjørg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149325/
https://www.ncbi.nlm.nih.gov/pubmed/32309448
http://dx.doi.org/10.1155/2020/7216863
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author Bratseth, Vibeke
Margeirsdottir, Hanna D.
Chiva-Blanch, Gemma
Heier, Martin
Solheim, Svein
Arnesen, Harald
Dahl-Jørgensen, Knut
Seljeflot, Ingebjørg
author_facet Bratseth, Vibeke
Margeirsdottir, Hanna D.
Chiva-Blanch, Gemma
Heier, Martin
Solheim, Svein
Arnesen, Harald
Dahl-Jørgensen, Knut
Seljeflot, Ingebjørg
author_sort Bratseth, Vibeke
collection PubMed
description BACKGROUND: Type 1 diabetes is a chronic disease including hyperglycemia and accelerated atherosclerosis, with high risk of micro- and macrovascular complications. Circulating microvesicles (cMVs) are procoagulant cell fragments shed during activation/apoptosis and discussed to be markers of vascular dysfunction and hypercoagulability. Limited knowledge exists on hypercoagulability in young diabetics. We aimed to investigate cMVs over a five-year period in children/adolescents with type 1 diabetes compared with controls and any associations with glycemic control and cardiovascular risk factors. We hypothesized increased shedding of cMVs in type 1 diabetes in response to vascular activation. METHODS: The cohort included type 1 diabetics (n = 40) and healthy controls (n = 40), mean age 14 years (range 11) at inclusion, randomly selected from the Norwegian Atherosclerosis and Childhood Diabetes (ACD) study. Citrated plasma was prepared and stored at -80°C until cMV analysis by flow cytometry. RESULTS: Comparable levels of Annexin V (AV(+)) cMVs were observed at inclusion. At five-year follow-up, total AV(+) cMVs were significantly lower in subjects with type 1 diabetes compared with controls; however, no significant differences were observed after adjusting for covariates. In the type 1 diabetes group, the total AV(+), tissue factor-expressing AV(+)/CD142(+), neutrophil-derived AV(+)/CD15(+) and AV(+)/CD45(+)/CD15(+), and endothelial-derived AV(+)/CD309(+) and CD309(+)/CD34(+) cMVs were inversely correlated with HbA1c (r = ‐0.437, r = ‐0.515, r = ‐0.575, r = ‐0.529, r = ‐0.416, and r = ‐0.445, respectively; all p ≤ 0.01), however, only at inclusion. No significant correlations with cardiovascular risk factors were observed. CONCLUSIONS: Children/adolescents with type 1 diabetes show similar levels of AV(+) cMVs as healthy controls and limited associations with glucose control. This indicates that our young diabetics on intensive insulin treatment have preserved vascular homeostasis and absence of procoagulant cMVs.
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spelling pubmed-71493252020-04-17 Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study Bratseth, Vibeke Margeirsdottir, Hanna D. Chiva-Blanch, Gemma Heier, Martin Solheim, Svein Arnesen, Harald Dahl-Jørgensen, Knut Seljeflot, Ingebjørg J Diabetes Res Research Article BACKGROUND: Type 1 diabetes is a chronic disease including hyperglycemia and accelerated atherosclerosis, with high risk of micro- and macrovascular complications. Circulating microvesicles (cMVs) are procoagulant cell fragments shed during activation/apoptosis and discussed to be markers of vascular dysfunction and hypercoagulability. Limited knowledge exists on hypercoagulability in young diabetics. We aimed to investigate cMVs over a five-year period in children/adolescents with type 1 diabetes compared with controls and any associations with glycemic control and cardiovascular risk factors. We hypothesized increased shedding of cMVs in type 1 diabetes in response to vascular activation. METHODS: The cohort included type 1 diabetics (n = 40) and healthy controls (n = 40), mean age 14 years (range 11) at inclusion, randomly selected from the Norwegian Atherosclerosis and Childhood Diabetes (ACD) study. Citrated plasma was prepared and stored at -80°C until cMV analysis by flow cytometry. RESULTS: Comparable levels of Annexin V (AV(+)) cMVs were observed at inclusion. At five-year follow-up, total AV(+) cMVs were significantly lower in subjects with type 1 diabetes compared with controls; however, no significant differences were observed after adjusting for covariates. In the type 1 diabetes group, the total AV(+), tissue factor-expressing AV(+)/CD142(+), neutrophil-derived AV(+)/CD15(+) and AV(+)/CD45(+)/CD15(+), and endothelial-derived AV(+)/CD309(+) and CD309(+)/CD34(+) cMVs were inversely correlated with HbA1c (r = ‐0.437, r = ‐0.515, r = ‐0.575, r = ‐0.529, r = ‐0.416, and r = ‐0.445, respectively; all p ≤ 0.01), however, only at inclusion. No significant correlations with cardiovascular risk factors were observed. CONCLUSIONS: Children/adolescents with type 1 diabetes show similar levels of AV(+) cMVs as healthy controls and limited associations with glucose control. This indicates that our young diabetics on intensive insulin treatment have preserved vascular homeostasis and absence of procoagulant cMVs. Hindawi 2020-03-30 /pmc/articles/PMC7149325/ /pubmed/32309448 http://dx.doi.org/10.1155/2020/7216863 Text en Copyright © 2020 Vibeke Bratseth et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bratseth, Vibeke
Margeirsdottir, Hanna D.
Chiva-Blanch, Gemma
Heier, Martin
Solheim, Svein
Arnesen, Harald
Dahl-Jørgensen, Knut
Seljeflot, Ingebjørg
Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title_full Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title_fullStr Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title_full_unstemmed Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title_short Annexin V(+) Microvesicles in Children and Adolescents with Type 1 Diabetes: A Prospective Cohort Study
title_sort annexin v(+) microvesicles in children and adolescents with type 1 diabetes: a prospective cohort study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149325/
https://www.ncbi.nlm.nih.gov/pubmed/32309448
http://dx.doi.org/10.1155/2020/7216863
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