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Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, myc...

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Autores principales: Sikandar, Aatika, Zhang, Mengyue, Wang, Yuanyuan, Zhu, Xiaofeng, Liu, Xiaoyu, Fan, Haiyan, Xuan, Yuanhu, Chen, Lijie, Duan, Yuxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149326/
https://www.ncbi.nlm.nih.gov/pubmed/32309009
http://dx.doi.org/10.1155/2020/3073906
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author Sikandar, Aatika
Zhang, Mengyue
Wang, Yuanyuan
Zhu, Xiaofeng
Liu, Xiaoyu
Fan, Haiyan
Xuan, Yuanhu
Chen, Lijie
Duan, Yuxi
author_facet Sikandar, Aatika
Zhang, Mengyue
Wang, Yuanyuan
Zhu, Xiaofeng
Liu, Xiaoyu
Fan, Haiyan
Xuan, Yuanhu
Chen, Lijie
Duan, Yuxi
author_sort Sikandar, Aatika
collection PubMed
description Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries.
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spelling pubmed-71493262020-04-17 Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum) Sikandar, Aatika Zhang, Mengyue Wang, Yuanyuan Zhu, Xiaofeng Liu, Xiaoyu Fan, Haiyan Xuan, Yuanhu Chen, Lijie Duan, Yuxi J Anal Methods Chem Research Article Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. The prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). The study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of flavonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. The outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23 mg·QE/g and 135.77 ± 1.14 mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. The analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. The obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries. Hindawi 2020-03-30 /pmc/articles/PMC7149326/ /pubmed/32309009 http://dx.doi.org/10.1155/2020/3073906 Text en Copyright © 2020 Aatika Sikandar et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sikandar, Aatika
Zhang, Mengyue
Wang, Yuanyuan
Zhu, Xiaofeng
Liu, Xiaoyu
Fan, Haiyan
Xuan, Yuanhu
Chen, Lijie
Duan, Yuxi
Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title_full Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title_fullStr Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title_full_unstemmed Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title_short Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)
title_sort mycochemical screening and analysis, antioxidant activity, and biochemical composition of fermentation strain snef1216 (penicillium chrysogenum)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149326/
https://www.ncbi.nlm.nih.gov/pubmed/32309009
http://dx.doi.org/10.1155/2020/3073906
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