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Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases

BACKGROUND: Neutrophil extracellular traps (NETs) are fibrous structures released from activated neutrophils. NET formation has been reported to be associated with acute respiratory distress syndrome (ARDS). However, there are no reports dealing with serial changes of NET formation in tracheal aspir...

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Autores principales: Ojima, Masahiro, Yamamoto, Norihisa, Hirose, Tomoya, Hamaguchi, Shigeto, Tasaki, Osamu, Kojima, Takashi, Tomono, Kazunori, Ogura, Hiroshi, Shimazu, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149861/
https://www.ncbi.nlm.nih.gov/pubmed/32308985
http://dx.doi.org/10.1186/s40560-020-00444-5
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author Ojima, Masahiro
Yamamoto, Norihisa
Hirose, Tomoya
Hamaguchi, Shigeto
Tasaki, Osamu
Kojima, Takashi
Tomono, Kazunori
Ogura, Hiroshi
Shimazu, Takeshi
author_facet Ojima, Masahiro
Yamamoto, Norihisa
Hirose, Tomoya
Hamaguchi, Shigeto
Tasaki, Osamu
Kojima, Takashi
Tomono, Kazunori
Ogura, Hiroshi
Shimazu, Takeshi
author_sort Ojima, Masahiro
collection PubMed
description BACKGROUND: Neutrophil extracellular traps (NETs) are fibrous structures released from activated neutrophils. NET formation has been reported to be associated with acute respiratory distress syndrome (ARDS). However, there are no reports dealing with serial changes of NET formation in tracheal aspirate of ARDS patients. CASE PRESENTATION: We report three cases of ARDS. Case 1 is a 69-year-old man with necrotizing fasciitis of the buttocks, case 2 is a 49-year-old woman with extensive burns (80% of total body surface), and case 3 is a 73-year-old woman with severe bacterial pneumonia. We found abundant expression of citrullinated histone H3 (Cit-H3) and the formation of NETs at the onset of ARDS in all cases. The amounts of Cit-H3 and NETs decreased with the amelioration of respiratory failure in cases 1 and 2. In case 2, the amounts of Cit-H3 and NETs increased with aggravation of infection and respiratory status. In case 3, the abundant expression of Cit-H3 and NETs persisted; the patient did not recover from ARDS and eventually died. Cit-H3 and NETs were found in tracheal aspirates even if the patients had no direct injury to the lung as in cases 1 and 2. CONCLUSIONS: In these three cases, the formation of NETs was observed in tracheal aspirate of patients with ARDS by either direct or indirect insults to the lung. The amount of NET formation changed dynamically over the clinical course of each patient.
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spelling pubmed-71498612020-04-18 Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases Ojima, Masahiro Yamamoto, Norihisa Hirose, Tomoya Hamaguchi, Shigeto Tasaki, Osamu Kojima, Takashi Tomono, Kazunori Ogura, Hiroshi Shimazu, Takeshi J Intensive Care Case Report BACKGROUND: Neutrophil extracellular traps (NETs) are fibrous structures released from activated neutrophils. NET formation has been reported to be associated with acute respiratory distress syndrome (ARDS). However, there are no reports dealing with serial changes of NET formation in tracheal aspirate of ARDS patients. CASE PRESENTATION: We report three cases of ARDS. Case 1 is a 69-year-old man with necrotizing fasciitis of the buttocks, case 2 is a 49-year-old woman with extensive burns (80% of total body surface), and case 3 is a 73-year-old woman with severe bacterial pneumonia. We found abundant expression of citrullinated histone H3 (Cit-H3) and the formation of NETs at the onset of ARDS in all cases. The amounts of Cit-H3 and NETs decreased with the amelioration of respiratory failure in cases 1 and 2. In case 2, the amounts of Cit-H3 and NETs increased with aggravation of infection and respiratory status. In case 3, the abundant expression of Cit-H3 and NETs persisted; the patient did not recover from ARDS and eventually died. Cit-H3 and NETs were found in tracheal aspirates even if the patients had no direct injury to the lung as in cases 1 and 2. CONCLUSIONS: In these three cases, the formation of NETs was observed in tracheal aspirate of patients with ARDS by either direct or indirect insults to the lung. The amount of NET formation changed dynamically over the clinical course of each patient. BioMed Central 2020-04-10 /pmc/articles/PMC7149861/ /pubmed/32308985 http://dx.doi.org/10.1186/s40560-020-00444-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Case Report
Ojima, Masahiro
Yamamoto, Norihisa
Hirose, Tomoya
Hamaguchi, Shigeto
Tasaki, Osamu
Kojima, Takashi
Tomono, Kazunori
Ogura, Hiroshi
Shimazu, Takeshi
Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title_full Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title_fullStr Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title_full_unstemmed Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title_short Serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
title_sort serial change of neutrophil extracellular traps in tracheal aspirate of patients with acute respiratory distress syndrome: report of three cases
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149861/
https://www.ncbi.nlm.nih.gov/pubmed/32308985
http://dx.doi.org/10.1186/s40560-020-00444-5
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