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A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a str...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Neuroscience Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149959/ https://www.ncbi.nlm.nih.gov/pubmed/32284838 http://dx.doi.org/10.32598/bcn.9.10.235 |
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author | Grüßer, Linda Blaumeiser-Debarry, Rosmarie Rossaint, Rolf Krings, Matthias Kremer, Benedikt Höllig, Anke Coburn, Mark |
author_facet | Grüßer, Linda Blaumeiser-Debarry, Rosmarie Rossaint, Rolf Krings, Matthias Kremer, Benedikt Höllig, Anke Coburn, Mark |
author_sort | Grüßer, Linda |
collection | PubMed |
description | INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied. METHODS: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study. RESULTS: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured. CONCLUSION: Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework. |
format | Online Article Text |
id | pubmed-7149959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Iranian Neuroscience Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-71499592020-04-13 A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model Grüßer, Linda Blaumeiser-Debarry, Rosmarie Rossaint, Rolf Krings, Matthias Kremer, Benedikt Höllig, Anke Coburn, Mark Basic Clin Neurosci Research Paper INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied. METHODS: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study. RESULTS: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured. CONCLUSION: Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework. Iranian Neuroscience Society 2019 2019-09-01 /pmc/articles/PMC7149959/ /pubmed/32284838 http://dx.doi.org/10.32598/bcn.9.10.235 Text en Copyright© 2019 Iranian Neuroscience Society This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
spellingShingle | Research Paper Grüßer, Linda Blaumeiser-Debarry, Rosmarie Rossaint, Rolf Krings, Matthias Kremer, Benedikt Höllig, Anke Coburn, Mark A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title | A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title_full | A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title_fullStr | A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title_full_unstemmed | A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title_short | A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model |
title_sort | 6-step approach to gain higher quality results from organotypic hippocampal brain slices in a traumatic brain injury model |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149959/ https://www.ncbi.nlm.nih.gov/pubmed/32284838 http://dx.doi.org/10.32598/bcn.9.10.235 |
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