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A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model

INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a str...

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Autores principales: Grüßer, Linda, Blaumeiser-Debarry, Rosmarie, Rossaint, Rolf, Krings, Matthias, Kremer, Benedikt, Höllig, Anke, Coburn, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Neuroscience Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149959/
https://www.ncbi.nlm.nih.gov/pubmed/32284838
http://dx.doi.org/10.32598/bcn.9.10.235
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author Grüßer, Linda
Blaumeiser-Debarry, Rosmarie
Rossaint, Rolf
Krings, Matthias
Kremer, Benedikt
Höllig, Anke
Coburn, Mark
author_facet Grüßer, Linda
Blaumeiser-Debarry, Rosmarie
Rossaint, Rolf
Krings, Matthias
Kremer, Benedikt
Höllig, Anke
Coburn, Mark
author_sort Grüßer, Linda
collection PubMed
description INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied. METHODS: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study. RESULTS: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured. CONCLUSION: Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework.
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spelling pubmed-71499592020-04-13 A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model Grüßer, Linda Blaumeiser-Debarry, Rosmarie Rossaint, Rolf Krings, Matthias Kremer, Benedikt Höllig, Anke Coburn, Mark Basic Clin Neurosci Research Paper INTRODUCTION: Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a setup. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied. METHODS: We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study. RESULTS: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured. CONCLUSION: Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework. Iranian Neuroscience Society 2019 2019-09-01 /pmc/articles/PMC7149959/ /pubmed/32284838 http://dx.doi.org/10.32598/bcn.9.10.235 Text en Copyright© 2019 Iranian Neuroscience Society This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Paper
Grüßer, Linda
Blaumeiser-Debarry, Rosmarie
Rossaint, Rolf
Krings, Matthias
Kremer, Benedikt
Höllig, Anke
Coburn, Mark
A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title_full A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title_fullStr A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title_full_unstemmed A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title_short A 6-Step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
title_sort 6-step approach to gain higher quality results from organotypic hippocampal brain slices in a traumatic brain injury model
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149959/
https://www.ncbi.nlm.nih.gov/pubmed/32284838
http://dx.doi.org/10.32598/bcn.9.10.235
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