Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample

We used metagenomics to analyze one sputum sample from a patient with symptoms of a respiratory infection that yielded negative results for all pathogens tested. We detected two viral genomes that could be assembled and showed sequence similarity to redondoviruses, a recently described group within...

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Autores principales: Lázaro-Perona, Fernando, Dahdouh, Elias, Román-Soto, Sergio, Jiménez-Rodríguez, Sonia, Rodríguez-Antolín, Carlos, de la Calle, Fernando, Agrifoglio, Alexander, Membrillo, Francisco Javier, García-Rodríguez, Julio, Mingorance, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150755/
https://www.ncbi.nlm.nih.gov/pubmed/32197299
http://dx.doi.org/10.3390/v12030327
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author Lázaro-Perona, Fernando
Dahdouh, Elias
Román-Soto, Sergio
Jiménez-Rodríguez, Sonia
Rodríguez-Antolín, Carlos
de la Calle, Fernando
Agrifoglio, Alexander
Membrillo, Francisco Javier
García-Rodríguez, Julio
Mingorance, Jesús
author_facet Lázaro-Perona, Fernando
Dahdouh, Elias
Román-Soto, Sergio
Jiménez-Rodríguez, Sonia
Rodríguez-Antolín, Carlos
de la Calle, Fernando
Agrifoglio, Alexander
Membrillo, Francisco Javier
García-Rodríguez, Julio
Mingorance, Jesús
author_sort Lázaro-Perona, Fernando
collection PubMed
description We used metagenomics to analyze one sputum sample from a patient with symptoms of a respiratory infection that yielded negative results for all pathogens tested. We detected two viral genomes that could be assembled and showed sequence similarity to redondoviruses, a recently described group within the CRESS-DNA viruses. One hundred sputum samples were screened for the presence of these viruses using specific primers. One sample was positive for the same two viruses, and another was positive for one of them. These findings raise questions about a possible role of redondoviruses in respiratory infections in humans.
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spelling pubmed-71507552020-04-20 Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample Lázaro-Perona, Fernando Dahdouh, Elias Román-Soto, Sergio Jiménez-Rodríguez, Sonia Rodríguez-Antolín, Carlos de la Calle, Fernando Agrifoglio, Alexander Membrillo, Francisco Javier García-Rodríguez, Julio Mingorance, Jesús Viruses Communication We used metagenomics to analyze one sputum sample from a patient with symptoms of a respiratory infection that yielded negative results for all pathogens tested. We detected two viral genomes that could be assembled and showed sequence similarity to redondoviruses, a recently described group within the CRESS-DNA viruses. One hundred sputum samples were screened for the presence of these viruses using specific primers. One sample was positive for the same two viruses, and another was positive for one of them. These findings raise questions about a possible role of redondoviruses in respiratory infections in humans. MDPI 2020-03-18 /pmc/articles/PMC7150755/ /pubmed/32197299 http://dx.doi.org/10.3390/v12030327 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Lázaro-Perona, Fernando
Dahdouh, Elias
Román-Soto, Sergio
Jiménez-Rodríguez, Sonia
Rodríguez-Antolín, Carlos
de la Calle, Fernando
Agrifoglio, Alexander
Membrillo, Francisco Javier
García-Rodríguez, Julio
Mingorance, Jesús
Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title_full Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title_fullStr Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title_full_unstemmed Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title_short Metagenomic Detection of Two Vientoviruses in a Human Sputum Sample
title_sort metagenomic detection of two vientoviruses in a human sputum sample
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150755/
https://www.ncbi.nlm.nih.gov/pubmed/32197299
http://dx.doi.org/10.3390/v12030327
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