Cargando…
Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection
Beet necrotic yellow vein virus (BNYVV) infections induce stunting and leaf curling, as well as root and floral developmental defects and leaf senescence in Nicotiana benthamiana. A microarray analysis with probes capable of detecting 1596 candidate microRNAs (miRNAs) was conducted to investigate di...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150760/ https://www.ncbi.nlm.nih.gov/pubmed/32178444 http://dx.doi.org/10.3390/v12030310 |
_version_ | 1783521091630661632 |
---|---|
author | Liu, Junying Fan, Huiyan Wang, Ying Han, Chenggui Wang, Xianbing Yu, Jialin Li, Dawei Zhang, Yongliang |
author_facet | Liu, Junying Fan, Huiyan Wang, Ying Han, Chenggui Wang, Xianbing Yu, Jialin Li, Dawei Zhang, Yongliang |
author_sort | Liu, Junying |
collection | PubMed |
description | Beet necrotic yellow vein virus (BNYVV) infections induce stunting and leaf curling, as well as root and floral developmental defects and leaf senescence in Nicotiana benthamiana. A microarray analysis with probes capable of detecting 1596 candidate microRNAs (miRNAs) was conducted to investigate differentially expressed miRNAs and their targets upon BNYVV infection of N. benthamiana plants. Eight species-specific miRNAs of N. benthamiana were identified. Comprehensive characterization of the N. benthamiana microRNA profile in response to the BNYVV infection revealed that 129 miRNAs were altered, including four species-specific miRNAs. The targets of the differentially expressed miRNAs were predicted accordingly. The expressions of miR164, 160, and 393 were up-regulated by BNYVV infection, and those of their target genes, NAC21/22, ARF17/18, and TIR, were down-regulated. GRF1, which is a target of miR396, was also down-regulated. Further genetic analysis of GRF1, by Tobacco rattle virus-induced gene silencing, assay confirmed the involvement of GRF1 in the symptom development during BNYVV infection. BNYVV infection also induced the up-regulation of miR168 and miR398. The miR398 was predicted to target umecyanin, and silencing of umecyanin could enhance plant resistance against viruses, suggesting the activation of primary defense response to BNYVV infection in N. benthamiana. These results provide a global profile of miRNA changes induced by BNYVV infection and enhance our understanding of the mechanisms underlying BNYVV pathogenesis. |
format | Online Article Text |
id | pubmed-7150760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71507602020-04-20 Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection Liu, Junying Fan, Huiyan Wang, Ying Han, Chenggui Wang, Xianbing Yu, Jialin Li, Dawei Zhang, Yongliang Viruses Article Beet necrotic yellow vein virus (BNYVV) infections induce stunting and leaf curling, as well as root and floral developmental defects and leaf senescence in Nicotiana benthamiana. A microarray analysis with probes capable of detecting 1596 candidate microRNAs (miRNAs) was conducted to investigate differentially expressed miRNAs and their targets upon BNYVV infection of N. benthamiana plants. Eight species-specific miRNAs of N. benthamiana were identified. Comprehensive characterization of the N. benthamiana microRNA profile in response to the BNYVV infection revealed that 129 miRNAs were altered, including four species-specific miRNAs. The targets of the differentially expressed miRNAs were predicted accordingly. The expressions of miR164, 160, and 393 were up-regulated by BNYVV infection, and those of their target genes, NAC21/22, ARF17/18, and TIR, were down-regulated. GRF1, which is a target of miR396, was also down-regulated. Further genetic analysis of GRF1, by Tobacco rattle virus-induced gene silencing, assay confirmed the involvement of GRF1 in the symptom development during BNYVV infection. BNYVV infection also induced the up-regulation of miR168 and miR398. The miR398 was predicted to target umecyanin, and silencing of umecyanin could enhance plant resistance against viruses, suggesting the activation of primary defense response to BNYVV infection in N. benthamiana. These results provide a global profile of miRNA changes induced by BNYVV infection and enhance our understanding of the mechanisms underlying BNYVV pathogenesis. MDPI 2020-03-12 /pmc/articles/PMC7150760/ /pubmed/32178444 http://dx.doi.org/10.3390/v12030310 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Junying Fan, Huiyan Wang, Ying Han, Chenggui Wang, Xianbing Yu, Jialin Li, Dawei Zhang, Yongliang Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title | Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title_full | Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title_fullStr | Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title_full_unstemmed | Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title_short | Genome-Wide microRNA Profiling Using Oligonucleotide Microarray Reveals Regulatory Networks of microRNAs in Nicotiana benthamiana During Beet Necrotic Yellow Vein Virus Infection |
title_sort | genome-wide microrna profiling using oligonucleotide microarray reveals regulatory networks of micrornas in nicotiana benthamiana during beet necrotic yellow vein virus infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150760/ https://www.ncbi.nlm.nih.gov/pubmed/32178444 http://dx.doi.org/10.3390/v12030310 |
work_keys_str_mv | AT liujunying genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT fanhuiyan genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT wangying genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT hanchenggui genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT wangxianbing genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT yujialin genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT lidawei genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection AT zhangyongliang genomewidemicrornaprofilingusingoligonucleotidemicroarrayrevealsregulatorynetworksofmicrornasinnicotianabenthamianaduringbeetnecroticyellowveinvirusinfection |