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Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer
Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150798/ https://www.ncbi.nlm.nih.gov/pubmed/32183214 http://dx.doi.org/10.3390/toxins12030179 |
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author | Jiménez-Cárcamo, Danae García, Carlos Contreras, Héctor R. |
author_facet | Jiménez-Cárcamo, Danae García, Carlos Contreras, Héctor R. |
author_sort | Jiménez-Cárcamo, Danae |
collection | PubMed |
description | Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhibitors, mainly type 1 protein phosphatase (PP1) and type 2A protein phosphatase (PP2A). Different effects have been reported at the cellular, molecular and genetic levels. In this study, changes in cell survival and cell mobility induced by OA, DTX-1 and DTX-2 were determined in epithelial cell lines of the colon and colon cancer. The cell viability results showed that tumoral cell lines were more resistant to toxins than the nontumoral cell line. The results of the functional assays for testing cell migration, evaluation of cell death and the expression of proteins associated with cell adhesion showed a dual effect of toxins since in the nontumoral cell line, a greater induction of cell death, presumably by anoikis, was detected. In the tumoral cell lines, there was an induction of a more aggressive phenotype characterized by increased resistance to toxins, increased migration and increased FAK activation. In tumoral cell lines of colon cancer, OA, DTX-1/DTX-2 induce a more aggressive phenotype. |
format | Online Article Text |
id | pubmed-7150798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71507982020-04-20 Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer Jiménez-Cárcamo, Danae García, Carlos Contreras, Héctor R. Toxins (Basel) Article Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhibitors, mainly type 1 protein phosphatase (PP1) and type 2A protein phosphatase (PP2A). Different effects have been reported at the cellular, molecular and genetic levels. In this study, changes in cell survival and cell mobility induced by OA, DTX-1 and DTX-2 were determined in epithelial cell lines of the colon and colon cancer. The cell viability results showed that tumoral cell lines were more resistant to toxins than the nontumoral cell line. The results of the functional assays for testing cell migration, evaluation of cell death and the expression of proteins associated with cell adhesion showed a dual effect of toxins since in the nontumoral cell line, a greater induction of cell death, presumably by anoikis, was detected. In the tumoral cell lines, there was an induction of a more aggressive phenotype characterized by increased resistance to toxins, increased migration and increased FAK activation. In tumoral cell lines of colon cancer, OA, DTX-1/DTX-2 induce a more aggressive phenotype. MDPI 2020-03-13 /pmc/articles/PMC7150798/ /pubmed/32183214 http://dx.doi.org/10.3390/toxins12030179 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jiménez-Cárcamo, Danae García, Carlos Contreras, Héctor R. Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title_full | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title_fullStr | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title_full_unstemmed | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title_short | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
title_sort | toxins of okadaic acid-group increase malignant properties in cells of colon cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150798/ https://www.ncbi.nlm.nih.gov/pubmed/32183214 http://dx.doi.org/10.3390/toxins12030179 |
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