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New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus

(1) Background: The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. Flavescence dorée is one of the most severely damaging diseases of Vitis vinifera and, consequently, a major threat to grape and wine production in several European...

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Autores principales: Ottati, Sara, Chiapello, Marco, Galetto, Luciana, Bosco, Domenico, Marzachì, Cristina, Abbà, Simona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150801/
https://www.ncbi.nlm.nih.gov/pubmed/32155753
http://dx.doi.org/10.3390/v12030287
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author Ottati, Sara
Chiapello, Marco
Galetto, Luciana
Bosco, Domenico
Marzachì, Cristina
Abbà, Simona
author_facet Ottati, Sara
Chiapello, Marco
Galetto, Luciana
Bosco, Domenico
Marzachì, Cristina
Abbà, Simona
author_sort Ottati, Sara
collection PubMed
description (1) Background: The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. Flavescence dorée is one of the most severely damaging diseases of Vitis vinifera and, consequently, a major threat to grape and wine production in several European countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on the environment. One possible solution is to exploit the largely unexplored genetic diversity of viruses infecting S. titanus as highly specific and environmentally benign tools for biological control. (2) Methods: A metatranscriptomic approach was adopted to identify viruses that may infect individuals caught in the wild in both its native (United States) and invasive (Europe) areas. Reverse transcription PCR was used to confirm their presence in RNA pools and explore their prevalence. (3) Results: We described nine new RNA viruses, including members of “Picorna-Calici”, “Permutotetra”, “Bunya-Arena”, “Reo”, “Partiti-Picobirna”, “Luteo-Sobemo” and “Toti-Chryso” clades. A marked difference in the diversity and abundance of the viral species was observed between the USA population and the European ones. (4) Conclusions: This work represents the first survey to assess the viral community of a phytoplasma insect vector. The possibility to exploit these naturally occurring viruses as specific and targeted biocontrol agents of S. titanus could be the answer to increasing demand for a more sustainable viticulture.
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spelling pubmed-71508012020-04-20 New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus Ottati, Sara Chiapello, Marco Galetto, Luciana Bosco, Domenico Marzachì, Cristina Abbà, Simona Viruses Article (1) Background: The leafhopper Scaphoideus titanus is the primary vector of Flavescence dorée phytoplasma (FDp) in European vineyards. Flavescence dorée is one of the most severely damaging diseases of Vitis vinifera and, consequently, a major threat to grape and wine production in several European countries. Control measures are compulsory, but they mainly involve large-scale insecticide treatments, with detrimental impacts on the environment. One possible solution is to exploit the largely unexplored genetic diversity of viruses infecting S. titanus as highly specific and environmentally benign tools for biological control. (2) Methods: A metatranscriptomic approach was adopted to identify viruses that may infect individuals caught in the wild in both its native (United States) and invasive (Europe) areas. Reverse transcription PCR was used to confirm their presence in RNA pools and explore their prevalence. (3) Results: We described nine new RNA viruses, including members of “Picorna-Calici”, “Permutotetra”, “Bunya-Arena”, “Reo”, “Partiti-Picobirna”, “Luteo-Sobemo” and “Toti-Chryso” clades. A marked difference in the diversity and abundance of the viral species was observed between the USA population and the European ones. (4) Conclusions: This work represents the first survey to assess the viral community of a phytoplasma insect vector. The possibility to exploit these naturally occurring viruses as specific and targeted biocontrol agents of S. titanus could be the answer to increasing demand for a more sustainable viticulture. MDPI 2020-03-06 /pmc/articles/PMC7150801/ /pubmed/32155753 http://dx.doi.org/10.3390/v12030287 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ottati, Sara
Chiapello, Marco
Galetto, Luciana
Bosco, Domenico
Marzachì, Cristina
Abbà, Simona
New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title_full New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title_fullStr New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title_full_unstemmed New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title_short New Viral Sequences Identified in the Flavescence Dorée Phytoplasma Vector Scaphoideus titanus
title_sort new viral sequences identified in the flavescence dorée phytoplasma vector scaphoideus titanus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150801/
https://www.ncbi.nlm.nih.gov/pubmed/32155753
http://dx.doi.org/10.3390/v12030287
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