Cargando…

Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2

Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed gene...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Hua, Deng, Xiwen, Zhou, Chuang, Wu, Wenda, Zhang, Haibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150952/
https://www.ncbi.nlm.nih.gov/pubmed/32183221
http://dx.doi.org/10.3390/toxins12030180
_version_ 1783521137290903552
author Zhang, Hua
Deng, Xiwen
Zhou, Chuang
Wu, Wenda
Zhang, Haibin
author_facet Zhang, Hua
Deng, Xiwen
Zhou, Chuang
Wu, Wenda
Zhang, Haibin
author_sort Zhang, Hua
collection PubMed
description Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed genes (DEGs) using RNA-seq and identified 320 upregulated genes and 160 downregulated genes. The enrichment pathways of these DEGs focused on immune-related pathways. DON induced proinflammatory gene expression, including cytokines, chemokines and other inflammation-related genes. DON increased IL1A, IL6 and TNF-α release and DON activated the phosphorylation of extracellular signal-regulated kinase-1 and-2 (ERK1/2), JUN N-terminal kinase (JNK) and p38 MAPK. A p38 inhibitor attenuated DON-induced IL6, TNF-α, CXCL2, CXCL8, IL12A, IL1A, CCL20, CCL4 and IL15 production, while an ERK1/2 inhibitor had only a small inhibitory effect on IL15 and IL6. An inhibitor of p38 MAPK decreased the release of IL1A, IL6 and TNF-α and an inhibitor of ERK1/2 partly attenuated protein levels of IL6. These data demonstrate that DON induces proinflammatory factor production in IPEC-J2 cells by activating p38 and ERK1/2.
format Online
Article
Text
id pubmed-7150952
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71509522020-04-20 Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2 Zhang, Hua Deng, Xiwen Zhou, Chuang Wu, Wenda Zhang, Haibin Toxins (Basel) Article Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed genes (DEGs) using RNA-seq and identified 320 upregulated genes and 160 downregulated genes. The enrichment pathways of these DEGs focused on immune-related pathways. DON induced proinflammatory gene expression, including cytokines, chemokines and other inflammation-related genes. DON increased IL1A, IL6 and TNF-α release and DON activated the phosphorylation of extracellular signal-regulated kinase-1 and-2 (ERK1/2), JUN N-terminal kinase (JNK) and p38 MAPK. A p38 inhibitor attenuated DON-induced IL6, TNF-α, CXCL2, CXCL8, IL12A, IL1A, CCL20, CCL4 and IL15 production, while an ERK1/2 inhibitor had only a small inhibitory effect on IL15 and IL6. An inhibitor of p38 MAPK decreased the release of IL1A, IL6 and TNF-α and an inhibitor of ERK1/2 partly attenuated protein levels of IL6. These data demonstrate that DON induces proinflammatory factor production in IPEC-J2 cells by activating p38 and ERK1/2. MDPI 2020-03-13 /pmc/articles/PMC7150952/ /pubmed/32183221 http://dx.doi.org/10.3390/toxins12030180 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Hua
Deng, Xiwen
Zhou, Chuang
Wu, Wenda
Zhang, Haibin
Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title_full Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title_fullStr Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title_full_unstemmed Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title_short Deoxynivalenol Induces Inflammation in IPEC-J2 Cells by Activating P38 Mapk And Erk1/2
title_sort deoxynivalenol induces inflammation in ipec-j2 cells by activating p38 mapk and erk1/2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150952/
https://www.ncbi.nlm.nih.gov/pubmed/32183221
http://dx.doi.org/10.3390/toxins12030180
work_keys_str_mv AT zhanghua deoxynivalenolinducesinflammationinipecj2cellsbyactivatingp38mapkanderk12
AT dengxiwen deoxynivalenolinducesinflammationinipecj2cellsbyactivatingp38mapkanderk12
AT zhouchuang deoxynivalenolinducesinflammationinipecj2cellsbyactivatingp38mapkanderk12
AT wuwenda deoxynivalenolinducesinflammationinipecj2cellsbyactivatingp38mapkanderk12
AT zhanghaibin deoxynivalenolinducesinflammationinipecj2cellsbyactivatingp38mapkanderk12