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Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects

The oxidant/antioxidant balance has been implicated in the pathophysiology of prostate cancer. We investigated oxidative damage and antioxidant status in high-risk prostate cancer subjects. Reduced glutathione (GSH) levels were measured in erythrocytes, 8-hydroxydeoxyguanosine (8-OHdG) in leukocytes...

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Autores principales: Shukla, Sanjeev, Srivastava, Janmejai K., Shankar, Eswar, Kanwal, Rajnee, Nawab, Akbar, Sharma, Haripaul, Bhaskaran, Natarajan, Ponsky, Lee E., Fu, Pingfu, MacLennan, Gregory T., Gupta, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151307/
https://www.ncbi.nlm.nih.gov/pubmed/32120827
http://dx.doi.org/10.3390/diagnostics10030126
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author Shukla, Sanjeev
Srivastava, Janmejai K.
Shankar, Eswar
Kanwal, Rajnee
Nawab, Akbar
Sharma, Haripaul
Bhaskaran, Natarajan
Ponsky, Lee E.
Fu, Pingfu
MacLennan, Gregory T.
Gupta, Sanjay
author_facet Shukla, Sanjeev
Srivastava, Janmejai K.
Shankar, Eswar
Kanwal, Rajnee
Nawab, Akbar
Sharma, Haripaul
Bhaskaran, Natarajan
Ponsky, Lee E.
Fu, Pingfu
MacLennan, Gregory T.
Gupta, Sanjay
author_sort Shukla, Sanjeev
collection PubMed
description The oxidant/antioxidant balance has been implicated in the pathophysiology of prostate cancer. We investigated oxidative damage and antioxidant status in high-risk prostate cancer subjects. Reduced glutathione (GSH) levels were measured in erythrocytes, 8-hydroxydeoxyguanosine (8-OHdG) in leukocytes and plasma levels of catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R), glutathione S-transferase (GST), superoxide dismutase (SOD), and lipid peroxide products were measured in high-risk and age-matched healthy subjects. Serum PSA levels were significantly higher (p < 0.0001) in high-risk subjects, whereas GST (p < 0.0001) and GSH (p < 0.002) were higher in healthy controls. Levels of 8-OHdG, an oxidized nucleoside of DNA, were significantly increased (p < 0.0001) in high-risk subjects. No marked difference in the levels of CAT (p = 0.237), GSH-Px (p = 0.74), GSH-R (p = 0.344), SOD (p = 0.109), and lipid peroxide products (p = 0129) were observed between two groups. Pearson’s correlation between GST and PSA (r = −0.69 (p < 0.0001)), GST and 8-OHdG (r = −0.62 (p < 0.0004)), GSH and 8-OHdG (r= −0.39 (p = 0.038)), and CAT and GSH-Px (r= −0.33 (p = 0.04)) were found to be negatively correlated, whereas 8-OHdG and PSA were positively associated (r= 0.57 (p < 0.002). These results indicate a significant role of oxidative damage in prostate carcinogenesis, particularly during the early stages of development. In conclusion, our data support the importance of antioxidant defense as a valuable diagnostic and/or prognostic marker in prostate cancer.
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spelling pubmed-71513072020-04-20 Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects Shukla, Sanjeev Srivastava, Janmejai K. Shankar, Eswar Kanwal, Rajnee Nawab, Akbar Sharma, Haripaul Bhaskaran, Natarajan Ponsky, Lee E. Fu, Pingfu MacLennan, Gregory T. Gupta, Sanjay Diagnostics (Basel) Article The oxidant/antioxidant balance has been implicated in the pathophysiology of prostate cancer. We investigated oxidative damage and antioxidant status in high-risk prostate cancer subjects. Reduced glutathione (GSH) levels were measured in erythrocytes, 8-hydroxydeoxyguanosine (8-OHdG) in leukocytes and plasma levels of catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-R), glutathione S-transferase (GST), superoxide dismutase (SOD), and lipid peroxide products were measured in high-risk and age-matched healthy subjects. Serum PSA levels were significantly higher (p < 0.0001) in high-risk subjects, whereas GST (p < 0.0001) and GSH (p < 0.002) were higher in healthy controls. Levels of 8-OHdG, an oxidized nucleoside of DNA, were significantly increased (p < 0.0001) in high-risk subjects. No marked difference in the levels of CAT (p = 0.237), GSH-Px (p = 0.74), GSH-R (p = 0.344), SOD (p = 0.109), and lipid peroxide products (p = 0129) were observed between two groups. Pearson’s correlation between GST and PSA (r = −0.69 (p < 0.0001)), GST and 8-OHdG (r = −0.62 (p < 0.0004)), GSH and 8-OHdG (r= −0.39 (p = 0.038)), and CAT and GSH-Px (r= −0.33 (p = 0.04)) were found to be negatively correlated, whereas 8-OHdG and PSA were positively associated (r= 0.57 (p < 0.002). These results indicate a significant role of oxidative damage in prostate carcinogenesis, particularly during the early stages of development. In conclusion, our data support the importance of antioxidant defense as a valuable diagnostic and/or prognostic marker in prostate cancer. MDPI 2020-02-27 /pmc/articles/PMC7151307/ /pubmed/32120827 http://dx.doi.org/10.3390/diagnostics10030126 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shukla, Sanjeev
Srivastava, Janmejai K.
Shankar, Eswar
Kanwal, Rajnee
Nawab, Akbar
Sharma, Haripaul
Bhaskaran, Natarajan
Ponsky, Lee E.
Fu, Pingfu
MacLennan, Gregory T.
Gupta, Sanjay
Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title_full Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title_fullStr Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title_full_unstemmed Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title_short Oxidative Stress and Antioxidant Status in High-Risk Prostate Cancer Subjects
title_sort oxidative stress and antioxidant status in high-risk prostate cancer subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151307/
https://www.ncbi.nlm.nih.gov/pubmed/32120827
http://dx.doi.org/10.3390/diagnostics10030126
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