Cargando…

Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces

In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelan...

Descripción completa

Detalles Bibliográficos
Autores principales: Zuppolini, Simona, Cruz-Maya, Iriczalli, Guarino, Vincenzo, Borriello, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151565/
https://www.ncbi.nlm.nih.gov/pubmed/32192126
http://dx.doi.org/10.3390/jfb11010019
_version_ 1783521280029360128
author Zuppolini, Simona
Cruz-Maya, Iriczalli
Guarino, Vincenzo
Borriello, Anna
author_facet Zuppolini, Simona
Cruz-Maya, Iriczalli
Guarino, Vincenzo
Borriello, Anna
author_sort Zuppolini, Simona
collection PubMed
description In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelanin polymer, was recognized as a suitable bio-interface coating. PDA was successfully used to improve adhesion due to the availability of copious functional groups for covalently immobilizing biomolecules and anchoring reactive species and ions. Recently, it has been demonstrated that PDA and its derivatives can be successfully used for the surface modification of implants interfaces to modulate in vitro cellular responses in order to enhance the in vivo functionality of biomedical implants (i.e., prosthesis). Herein, we propose the development of multifunctional scaffolds based on polyε–caprolactone (PCL) electrospun fibers coated with PDA via electro fluid dynamic methods, by optimizing polymerization/oxidation reactions capable of driving PDA self–assembly, and, ultimately, investigating the effects on cell response. Morphological analyses have confirmed the possibility to obtain different surface topographies as a function of the coating process while in vitro studies proved the ability of PDA coating to interact with cells no compromising in vitro viability. In perspective, in vitro conductive properties of fibers will be further investigated in order to validate their promising use as bioconductive interfaces for tissue engineering applications.
format Online
Article
Text
id pubmed-7151565
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71515652020-04-20 Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces Zuppolini, Simona Cruz-Maya, Iriczalli Guarino, Vincenzo Borriello, Anna J Funct Biomater Article In recent years, mussel adhesive proteins have attracted much attention because they can form strong adhesive interface interactions with various substrates in a wet environment. Inspired by their catechol- and amine-based molecular structure, polydopamine (PDA), a dopamine derived synthetic eumelanin polymer, was recognized as a suitable bio-interface coating. PDA was successfully used to improve adhesion due to the availability of copious functional groups for covalently immobilizing biomolecules and anchoring reactive species and ions. Recently, it has been demonstrated that PDA and its derivatives can be successfully used for the surface modification of implants interfaces to modulate in vitro cellular responses in order to enhance the in vivo functionality of biomedical implants (i.e., prosthesis). Herein, we propose the development of multifunctional scaffolds based on polyε–caprolactone (PCL) electrospun fibers coated with PDA via electro fluid dynamic methods, by optimizing polymerization/oxidation reactions capable of driving PDA self–assembly, and, ultimately, investigating the effects on cell response. Morphological analyses have confirmed the possibility to obtain different surface topographies as a function of the coating process while in vitro studies proved the ability of PDA coating to interact with cells no compromising in vitro viability. In perspective, in vitro conductive properties of fibers will be further investigated in order to validate their promising use as bioconductive interfaces for tissue engineering applications. MDPI 2020-03-17 /pmc/articles/PMC7151565/ /pubmed/32192126 http://dx.doi.org/10.3390/jfb11010019 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zuppolini, Simona
Cruz-Maya, Iriczalli
Guarino, Vincenzo
Borriello, Anna
Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title_full Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title_fullStr Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title_full_unstemmed Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title_short Optimization of Polydopamine Coatings onto Poly–ε–Caprolactone Electrospun Fibers for the Fabrication of Bio-Electroconductive Interfaces
title_sort optimization of polydopamine coatings onto poly–ε–caprolactone electrospun fibers for the fabrication of bio-electroconductive interfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151565/
https://www.ncbi.nlm.nih.gov/pubmed/32192126
http://dx.doi.org/10.3390/jfb11010019
work_keys_str_mv AT zuppolinisimona optimizationofpolydopaminecoatingsontopolyecaprolactoneelectrospunfibersforthefabricationofbioelectroconductiveinterfaces
AT cruzmayairiczalli optimizationofpolydopaminecoatingsontopolyecaprolactoneelectrospunfibersforthefabricationofbioelectroconductiveinterfaces
AT guarinovincenzo optimizationofpolydopaminecoatingsontopolyecaprolactoneelectrospunfibersforthefabricationofbioelectroconductiveinterfaces
AT borrielloanna optimizationofpolydopaminecoatingsontopolyecaprolactoneelectrospunfibersforthefabricationofbioelectroconductiveinterfaces