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Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury

The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from B. tetraphylla (BT) leaf ext...

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Autores principales: Silva, Tiago Fonseca, Cavalcanti Filho, José Robson Neves, Barreto Fonsêca, Mariana Mirelle Lima, dos Santos, Natalia Medeiros, Barbosa da Silva, Ana Carolina, Zagmignan, Adrielle, Abreu, Afonso Gomes, Sant’Anna da Silva, Ana Paula, Lima, Vera Lúcia de Menezes, da Silva, Nicácio Henrique, Dutra, Lívia Macedo, Almeida, Jackson Roberto Guedes da Silva, da Silva, Márcia Vanusa, Correia, Maria Tereza dos Santos, Nascimento da Silva, Luís Cláudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151707/
https://www.ncbi.nlm.nih.gov/pubmed/32188166
http://dx.doi.org/10.3390/ph13030046
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author Silva, Tiago Fonseca
Cavalcanti Filho, José Robson Neves
Barreto Fonsêca, Mariana Mirelle Lima
dos Santos, Natalia Medeiros
Barbosa da Silva, Ana Carolina
Zagmignan, Adrielle
Abreu, Afonso Gomes
Sant’Anna da Silva, Ana Paula
Lima, Vera Lúcia de Menezes
da Silva, Nicácio Henrique
Dutra, Lívia Macedo
Almeida, Jackson Roberto Guedes da Silva
da Silva, Márcia Vanusa
Correia, Maria Tereza dos Santos
Nascimento da Silva, Luís Cláudio
author_facet Silva, Tiago Fonseca
Cavalcanti Filho, José Robson Neves
Barreto Fonsêca, Mariana Mirelle Lima
dos Santos, Natalia Medeiros
Barbosa da Silva, Ana Carolina
Zagmignan, Adrielle
Abreu, Afonso Gomes
Sant’Anna da Silva, Ana Paula
Lima, Vera Lúcia de Menezes
da Silva, Nicácio Henrique
Dutra, Lívia Macedo
Almeida, Jackson Roberto Guedes da Silva
da Silva, Márcia Vanusa
Correia, Maria Tereza dos Santos
Nascimento da Silva, Luís Cláudio
author_sort Silva, Tiago Fonseca
collection PubMed
description The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from B. tetraphylla (BT) leaf extracts. In vitro methods (DPPH and ABTS assays; determination of phenolic and flavonoid contents) were used to select products derived from B. tetraphylla with high antioxidant action. Then, the samples with the highest potentials were evaluated in a model of injury based on the inoculation of a lethal dose of heat-inactivated Escherichia coli in Tenebrio molitor larvae. Due to its higher antioxidant properties, the methanolic extract (BTME) was chosen to be fractionated using Sephadex LH-20 column-based chromatography. Two fractions from BTME (BTFC and BTFD) were the most active fractions. Pre-treatment with these fractions protected larvae of T. molitor from the stress induced by inoculation of heat-inactivated E. coli. Similarly, BTFC and BTFD increased the lifespan of larvae infected with a lethal dose of enteroaggregative E. coli 042. NMR data indicated the presence of aliphatic compounds (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds). These findings suggested that products derived from B. tetraphylla leaves are promising candidates for the development of antioxidant and anti-infective agents able to treat oxidative-related dysfunctions.
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spelling pubmed-71517072020-04-20 Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury Silva, Tiago Fonseca Cavalcanti Filho, José Robson Neves Barreto Fonsêca, Mariana Mirelle Lima dos Santos, Natalia Medeiros Barbosa da Silva, Ana Carolina Zagmignan, Adrielle Abreu, Afonso Gomes Sant’Anna da Silva, Ana Paula Lima, Vera Lúcia de Menezes da Silva, Nicácio Henrique Dutra, Lívia Macedo Almeida, Jackson Roberto Guedes da Silva da Silva, Márcia Vanusa Correia, Maria Tereza dos Santos Nascimento da Silva, Luís Cláudio Pharmaceuticals (Basel) Article The relevance of oxidative stress in the pathogenesis of several diseases (including inflammatory disorders) has traditionally led to the search for new sources of antioxidant compounds. In this work, we report the selection of fractions with high antioxidant action from B. tetraphylla (BT) leaf extracts. In vitro methods (DPPH and ABTS assays; determination of phenolic and flavonoid contents) were used to select products derived from B. tetraphylla with high antioxidant action. Then, the samples with the highest potentials were evaluated in a model of injury based on the inoculation of a lethal dose of heat-inactivated Escherichia coli in Tenebrio molitor larvae. Due to its higher antioxidant properties, the methanolic extract (BTME) was chosen to be fractionated using Sephadex LH-20 column-based chromatography. Two fractions from BTME (BTFC and BTFD) were the most active fractions. Pre-treatment with these fractions protected larvae of T. molitor from the stress induced by inoculation of heat-inactivated E. coli. Similarly, BTFC and BTFD increased the lifespan of larvae infected with a lethal dose of enteroaggregative E. coli 042. NMR data indicated the presence of aliphatic compounds (terpenes, fatty acids, carbohydrates) and aromatic compounds (phenolic compounds). These findings suggested that products derived from B. tetraphylla leaves are promising candidates for the development of antioxidant and anti-infective agents able to treat oxidative-related dysfunctions. MDPI 2020-03-16 /pmc/articles/PMC7151707/ /pubmed/32188166 http://dx.doi.org/10.3390/ph13030046 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Tiago Fonseca
Cavalcanti Filho, José Robson Neves
Barreto Fonsêca, Mariana Mirelle Lima
dos Santos, Natalia Medeiros
Barbosa da Silva, Ana Carolina
Zagmignan, Adrielle
Abreu, Afonso Gomes
Sant’Anna da Silva, Ana Paula
Lima, Vera Lúcia de Menezes
da Silva, Nicácio Henrique
Dutra, Lívia Macedo
Almeida, Jackson Roberto Guedes da Silva
da Silva, Márcia Vanusa
Correia, Maria Tereza dos Santos
Nascimento da Silva, Luís Cláudio
Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title_full Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title_fullStr Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title_full_unstemmed Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title_short Products Derived from Buchenavia tetraphylla Leaves Have In Vitro Antioxidant Activity and Protect Tenebrio molitor Larvae against Escherichia coli-Induced Injury
title_sort products derived from buchenavia tetraphylla leaves have in vitro antioxidant activity and protect tenebrio molitor larvae against escherichia coli-induced injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151707/
https://www.ncbi.nlm.nih.gov/pubmed/32188166
http://dx.doi.org/10.3390/ph13030046
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