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A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney

In a previously published report we detailed an in situ method to quantify cell death in the renal cortex by perfusing the cell membrane impermeable fluorochrome, ethidium homodimer in situ. The objective of the present study was to use this in situ viability assay to examine cell death following th...

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Autores principales: Edwards, Joshua, Kowal, Michael, VanDreel, Aaron, Lamar, Peter, Prozialeck, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151727/
https://www.ncbi.nlm.nih.gov/pubmed/31968633
http://dx.doi.org/10.3390/toxics8010004
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author Edwards, Joshua
Kowal, Michael
VanDreel, Aaron
Lamar, Peter
Prozialeck, Walter
author_facet Edwards, Joshua
Kowal, Michael
VanDreel, Aaron
Lamar, Peter
Prozialeck, Walter
author_sort Edwards, Joshua
collection PubMed
description In a previously published report we detailed an in situ method to quantify cell death in the renal cortex by perfusing the cell membrane impermeable fluorochrome, ethidium homodimer in situ. The objective of the present study was to use this in situ viability assay to examine cell death following the administration of nephrotoxic drugs known to produce cell death and/or injury in specific segments of the nephron. Male Sprague/Dawley rats were treated with the following nephrotoxicants: Gentamicin, amphotericin-B, and indomethacin. Results of the in situ viability assay indicated that gentamicin and amphotericin-B treatment caused cell death localized in the kidney cortex and medulla, respectively. The urinary biomarker kidney injury molecule—1 (Kim-1) showed significant increases in both gentamicin (20 fold increase) and amphotericin-B-treated (9.2 fold increase) animals. Urinary alpha glutathione-S-transferase (GST) showed significant increases for gentamicin (6.2 fold increase) only and mu GST for amphotericin-B-treated (19.1 fold increase) animals only. These results show that this in situ viability assay provides a sensitive method to identify cell death in different regions of the kidney. Furthermore, urinary alpha GST and mu GST are specific for proximal and distal tubule injury, respectively; urinary Kim-1 demonstrated greater sensitivity to both proximal and distal tubule injury.
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spelling pubmed-71517272020-04-20 A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney Edwards, Joshua Kowal, Michael VanDreel, Aaron Lamar, Peter Prozialeck, Walter Toxics Article In a previously published report we detailed an in situ method to quantify cell death in the renal cortex by perfusing the cell membrane impermeable fluorochrome, ethidium homodimer in situ. The objective of the present study was to use this in situ viability assay to examine cell death following the administration of nephrotoxic drugs known to produce cell death and/or injury in specific segments of the nephron. Male Sprague/Dawley rats were treated with the following nephrotoxicants: Gentamicin, amphotericin-B, and indomethacin. Results of the in situ viability assay indicated that gentamicin and amphotericin-B treatment caused cell death localized in the kidney cortex and medulla, respectively. The urinary biomarker kidney injury molecule—1 (Kim-1) showed significant increases in both gentamicin (20 fold increase) and amphotericin-B-treated (9.2 fold increase) animals. Urinary alpha glutathione-S-transferase (GST) showed significant increases for gentamicin (6.2 fold increase) only and mu GST for amphotericin-B-treated (19.1 fold increase) animals only. These results show that this in situ viability assay provides a sensitive method to identify cell death in different regions of the kidney. Furthermore, urinary alpha GST and mu GST are specific for proximal and distal tubule injury, respectively; urinary Kim-1 demonstrated greater sensitivity to both proximal and distal tubule injury. MDPI 2020-01-20 /pmc/articles/PMC7151727/ /pubmed/31968633 http://dx.doi.org/10.3390/toxics8010004 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Edwards, Joshua
Kowal, Michael
VanDreel, Aaron
Lamar, Peter
Prozialeck, Walter
A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title_full A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title_fullStr A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title_full_unstemmed A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title_short A Method for the Evaluation of Site-Specific Nephrotoxic Injury in the Intact Rat Kidney
title_sort method for the evaluation of site-specific nephrotoxic injury in the intact rat kidney
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151727/
https://www.ncbi.nlm.nih.gov/pubmed/31968633
http://dx.doi.org/10.3390/toxics8010004
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