1.12 Labeling Techniques

The current status of isotope-assisted multidimensional nuclear magnetic resonance (NMR) spectroscopy for protein structural studies is reviewed. After introducing various classic isotope labeling methods, some new emerging technologies, such as the stereo-array isotope labeling method, are describe...

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Detalles Bibliográficos
Autores principales: Takeda, M., Kainosho, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151895/
http://dx.doi.org/10.1016/B978-0-12-374920-8.00116-8
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author Takeda, M.
Kainosho, M.
author_facet Takeda, M.
Kainosho, M.
author_sort Takeda, M.
collection PubMed
description The current status of isotope-assisted multidimensional nuclear magnetic resonance (NMR) spectroscopy for protein structural studies is reviewed. After introducing various classic isotope labeling methods, some new emerging technologies, such as the stereo-array isotope labeling method, are described. The concomitant development of advanced stable isotope labeling strategies, NMR instrumentation, sophisticated NMR measurements, spectral analysis, and structural calculation algorithms is essential to overcome the current limitations restricting the use of protein NMR spectroscopy.
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spelling pubmed-71518952020-04-13 1.12 Labeling Techniques Takeda, M. Kainosho, M. Comprehensive Biophysics Article The current status of isotope-assisted multidimensional nuclear magnetic resonance (NMR) spectroscopy for protein structural studies is reviewed. After introducing various classic isotope labeling methods, some new emerging technologies, such as the stereo-array isotope labeling method, are described. The concomitant development of advanced stable isotope labeling strategies, NMR instrumentation, sophisticated NMR measurements, spectral analysis, and structural calculation algorithms is essential to overcome the current limitations restricting the use of protein NMR spectroscopy. 2012 2012-05-03 /pmc/articles/PMC7151895/ http://dx.doi.org/10.1016/B978-0-12-374920-8.00116-8 Text en Copyright © 2012 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Takeda, M.
Kainosho, M.
1.12 Labeling Techniques
title 1.12 Labeling Techniques
title_full 1.12 Labeling Techniques
title_fullStr 1.12 Labeling Techniques
title_full_unstemmed 1.12 Labeling Techniques
title_short 1.12 Labeling Techniques
title_sort 1.12 labeling techniques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7151895/
http://dx.doi.org/10.1016/B978-0-12-374920-8.00116-8
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