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Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microsco...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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1981
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152140/ http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3 |
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author | Rodgers, F.G. |
author_facet | Rodgers, F.G. |
author_sort | Rodgers, F.G. |
collection | PubMed |
description | Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microscopy. By all three methods, a similar content and range of viruses were detected, but the hydrogel technique presented some distinct advantages. Factors influencing fluid absorption from faecal extracts are outlined. |
format | Online Article Text |
id | pubmed-7152140 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1981 |
record_format | MEDLINE/PubMed |
spelling | pubmed-71521402020-04-13 Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis Rodgers, F.G. Viruses and Wastewater Treatment Article Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microscopy. By all three methods, a similar content and range of viruses were detected, but the hydrogel technique presented some distinct advantages. Factors influencing fluid absorption from faecal extracts are outlined. 1981 2013-11-17 /pmc/articles/PMC7152140/ http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3 Text en © 1981 Pergamon Press Ltd Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Rodgers, F.G. Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title | Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title_full | Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title_fullStr | Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title_full_unstemmed | Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title_short | Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis |
title_sort | concentration of viruses in faecal samples from patients with gastroenteritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152140/ http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3 |
work_keys_str_mv | AT rodgersfg concentrationofvirusesinfaecalsamplesfrompatientswithgastroenteritis |