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Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis

Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microsco...

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Detalles Bibliográficos
Autor principal: Rodgers, F.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152140/
http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3
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author Rodgers, F.G.
author_facet Rodgers, F.G.
author_sort Rodgers, F.G.
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description Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microscopy. By all three methods, a similar content and range of viruses were detected, but the hydrogel technique presented some distinct advantages. Factors influencing fluid absorption from faecal extracts are outlined.
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spelling pubmed-71521402020-04-13 Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis Rodgers, F.G. Viruses and Wastewater Treatment Article Faecal samples from 100 patients with gastroenteritis were examined by three methods; a) fluid absorption with polyacrylamide hydrogel, b) ammonium sulphate precipitation and c) ultracentrifugation to compare the concentration of virus particles before examination by negative stain electron microscopy. By all three methods, a similar content and range of viruses were detected, but the hydrogel technique presented some distinct advantages. Factors influencing fluid absorption from faecal extracts are outlined. 1981 2013-11-17 /pmc/articles/PMC7152140/ http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3 Text en © 1981 Pergamon Press Ltd Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Rodgers, F.G.
Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title_full Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title_fullStr Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title_full_unstemmed Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title_short Concentration of Viruses in Faecal Samples from Patients with Gastroenteritis
title_sort concentration of viruses in faecal samples from patients with gastroenteritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152140/
http://dx.doi.org/10.1016/B978-0-08-026401-1.50006-3
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