Cargando…
A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice
Heat stress induces misfolded protein accumulation in endoplasmic reticulum (ER), which initiates the unfolded protein response (UPR) in plants. Previous work has demonstrated the important role of a rice ER membrane‐associated transcription factor OsbZIP74 (also known as OsbZIP50) in UPR. However,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152603/ https://www.ncbi.nlm.nih.gov/pubmed/31733092 http://dx.doi.org/10.1111/pbi.13297 |
_version_ | 1783521513214836736 |
---|---|
author | Liu, Xue‐Huan Lyu, Yu‐Shu Yang, Weiping Yang, Zheng‐Ting Lu, Sun‐Jie Liu, Jian‐Xiang |
author_facet | Liu, Xue‐Huan Lyu, Yu‐Shu Yang, Weiping Yang, Zheng‐Ting Lu, Sun‐Jie Liu, Jian‐Xiang |
author_sort | Liu, Xue‐Huan |
collection | PubMed |
description | Heat stress induces misfolded protein accumulation in endoplasmic reticulum (ER), which initiates the unfolded protein response (UPR) in plants. Previous work has demonstrated the important role of a rice ER membrane‐associated transcription factor OsbZIP74 (also known as OsbZIP50) in UPR. However, how OsbZIP74 and other membrane‐associated transcription factors are involved in heat stress tolerance in rice is not reported. In the current study, we discovered that OsNTL3 is required for heat stress tolerance in rice. OsNTL3 is constitutively expressed and up‐regulated by heat and ER stresses. OsNTL3 encodes a NAC transcription factor with a predicted C‐terminal transmembrane domain. GFP‐OsNTL3 relocates from plasma membrane to nucleus in response to heat stress and ER stress inducers. Loss‐of‐function mutation of OsNTL3 confers heat sensitivity while inducible expression of the truncated form of OsNTL3 without the transmembrane domain increases heat tolerance in rice seedlings. RNA‐Seq analysis revealed that OsNTL3 regulates the expression of genes involved in ER protein folding and other processes. Interestingly, OsNTL3 directly binds to OsbZIP74 promoter and regulates its expression in response to heat stress. In turn, up‐regulation of OsNTL3 by heat stress is dependent on OsbZIP74. Thus, our work reveals the important role of OsNTL3 in thermotolerance, and a regulatory circuit mediated by OsbZIP74 and OsNTL3 in communications among ER, plasma membrane and nucleus under heat stress conditions. |
format | Online Article Text |
id | pubmed-7152603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71526032020-04-14 A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice Liu, Xue‐Huan Lyu, Yu‐Shu Yang, Weiping Yang, Zheng‐Ting Lu, Sun‐Jie Liu, Jian‐Xiang Plant Biotechnol J Research Articles Heat stress induces misfolded protein accumulation in endoplasmic reticulum (ER), which initiates the unfolded protein response (UPR) in plants. Previous work has demonstrated the important role of a rice ER membrane‐associated transcription factor OsbZIP74 (also known as OsbZIP50) in UPR. However, how OsbZIP74 and other membrane‐associated transcription factors are involved in heat stress tolerance in rice is not reported. In the current study, we discovered that OsNTL3 is required for heat stress tolerance in rice. OsNTL3 is constitutively expressed and up‐regulated by heat and ER stresses. OsNTL3 encodes a NAC transcription factor with a predicted C‐terminal transmembrane domain. GFP‐OsNTL3 relocates from plasma membrane to nucleus in response to heat stress and ER stress inducers. Loss‐of‐function mutation of OsNTL3 confers heat sensitivity while inducible expression of the truncated form of OsNTL3 without the transmembrane domain increases heat tolerance in rice seedlings. RNA‐Seq analysis revealed that OsNTL3 regulates the expression of genes involved in ER protein folding and other processes. Interestingly, OsNTL3 directly binds to OsbZIP74 promoter and regulates its expression in response to heat stress. In turn, up‐regulation of OsNTL3 by heat stress is dependent on OsbZIP74. Thus, our work reveals the important role of OsNTL3 in thermotolerance, and a regulatory circuit mediated by OsbZIP74 and OsNTL3 in communications among ER, plasma membrane and nucleus under heat stress conditions. John Wiley and Sons Inc. 2019-12-06 2020-05 /pmc/articles/PMC7152603/ /pubmed/31733092 http://dx.doi.org/10.1111/pbi.13297 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Liu, Xue‐Huan Lyu, Yu‐Shu Yang, Weiping Yang, Zheng‐Ting Lu, Sun‐Jie Liu, Jian‐Xiang A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title | A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title_full | A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title_fullStr | A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title_full_unstemmed | A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title_short | A membrane‐associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice |
title_sort | membrane‐associated nac transcription factor osntl3 is involved in thermotolerance in rice |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152603/ https://www.ncbi.nlm.nih.gov/pubmed/31733092 http://dx.doi.org/10.1111/pbi.13297 |
work_keys_str_mv | AT liuxuehuan amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT lyuyushu amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT yangweiping amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT yangzhengting amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT lusunjie amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT liujianxiang amembraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT liuxuehuan membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT lyuyushu membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT yangweiping membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT yangzhengting membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT lusunjie membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice AT liujianxiang membraneassociatednactranscriptionfactorosntl3isinvolvedinthermotoleranceinrice |