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Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1

Boron (B) deficiency is one of the major causes of growth inhibition and yield reduction in Brassica napus (B. napus). However, the molecular mechanisms of low B adaptation in B. napus are largely unknown. Here, fifty‐one BnaWRKY transcription factors were identified as responsive to B deficiency in...

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Autores principales: Feng, Yingna, Cui, Rui, Wang, Sheliang, He, Mingliang, Hua, Yingpeng, Shi, Lei, Ye, Xiangsheng, Xu, Fangsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152615/
https://www.ncbi.nlm.nih.gov/pubmed/31705705
http://dx.doi.org/10.1111/pbi.13288
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author Feng, Yingna
Cui, Rui
Wang, Sheliang
He, Mingliang
Hua, Yingpeng
Shi, Lei
Ye, Xiangsheng
Xu, Fangsen
author_facet Feng, Yingna
Cui, Rui
Wang, Sheliang
He, Mingliang
Hua, Yingpeng
Shi, Lei
Ye, Xiangsheng
Xu, Fangsen
author_sort Feng, Yingna
collection PubMed
description Boron (B) deficiency is one of the major causes of growth inhibition and yield reduction in Brassica napus (B. napus). However, the molecular mechanisms of low B adaptation in B. napus are largely unknown. Here, fifty‐one BnaWRKY transcription factors were identified as responsive to B deficiency in B. napus, in which BnaAn.WRKY26, BnaA9.WRKY47, BnaA1.WKRY53 and BnaCn.WRKY57 were tested in yeast one‐hybrid assays and showed strong binding activity with conserved sequences containing a W box in the promoters of the B transport‐related genes BnaNIP5;1s and BnaBOR1s. Green fluorescent protein fused to the target protein demonstrated the nuclear localization of BnaA9.WRKY47. CRISPR/Cas9‐mediated knockout lines of BnaA9.WRKY47 in B. napus had increased sensitivity to low B and lower contents of B than wild‐type plants. In contrast, overexpression of BnaA9.WRKY47 enhanced the adaptation to low B with higher B contents in tissues than in wild‐type plants. Consistent with the phenotypic response and B accumulation in these transgenic lines, the transcription activity of BnaA3.NIP5;1, a B efficiency candidate gene, was decreased in the knockout lines but was significantly increased in the overexpressing lines under low B conditions. Electrophoretic mobility shift assays, transient expression experiments in tobacco and in situ hybridizations showed that BnaA9.WRKY47 directly activated BnaA3.NIP5;1 expression through binding to the specific cis‐element. Taken together, our findings support BnaWRKYs as new participants in response to low B, and BnaA9.WRKY47 contributes to the adaptation of B. napus to B deficiency through up‐regulating BnaA3.NIP5;1 expression to facilitate efficient B uptake.
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spelling pubmed-71526152020-04-14 Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1 Feng, Yingna Cui, Rui Wang, Sheliang He, Mingliang Hua, Yingpeng Shi, Lei Ye, Xiangsheng Xu, Fangsen Plant Biotechnol J Research Articles Boron (B) deficiency is one of the major causes of growth inhibition and yield reduction in Brassica napus (B. napus). However, the molecular mechanisms of low B adaptation in B. napus are largely unknown. Here, fifty‐one BnaWRKY transcription factors were identified as responsive to B deficiency in B. napus, in which BnaAn.WRKY26, BnaA9.WRKY47, BnaA1.WKRY53 and BnaCn.WRKY57 were tested in yeast one‐hybrid assays and showed strong binding activity with conserved sequences containing a W box in the promoters of the B transport‐related genes BnaNIP5;1s and BnaBOR1s. Green fluorescent protein fused to the target protein demonstrated the nuclear localization of BnaA9.WRKY47. CRISPR/Cas9‐mediated knockout lines of BnaA9.WRKY47 in B. napus had increased sensitivity to low B and lower contents of B than wild‐type plants. In contrast, overexpression of BnaA9.WRKY47 enhanced the adaptation to low B with higher B contents in tissues than in wild‐type plants. Consistent with the phenotypic response and B accumulation in these transgenic lines, the transcription activity of BnaA3.NIP5;1, a B efficiency candidate gene, was decreased in the knockout lines but was significantly increased in the overexpressing lines under low B conditions. Electrophoretic mobility shift assays, transient expression experiments in tobacco and in situ hybridizations showed that BnaA9.WRKY47 directly activated BnaA3.NIP5;1 expression through binding to the specific cis‐element. Taken together, our findings support BnaWRKYs as new participants in response to low B, and BnaA9.WRKY47 contributes to the adaptation of B. napus to B deficiency through up‐regulating BnaA3.NIP5;1 expression to facilitate efficient B uptake. John Wiley and Sons Inc. 2019-11-19 2020-05 /pmc/articles/PMC7152615/ /pubmed/31705705 http://dx.doi.org/10.1111/pbi.13288 Text en © 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Feng, Yingna
Cui, Rui
Wang, Sheliang
He, Mingliang
Hua, Yingpeng
Shi, Lei
Ye, Xiangsheng
Xu, Fangsen
Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title_full Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title_fullStr Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title_full_unstemmed Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title_short Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up‐regulating the boric acid channel gene BnaA3.NIP5;1
title_sort transcription factor bnaa9.wrky47 contributes to the adaptation of brassica napus to low boron stress by up‐regulating the boric acid channel gene bnaa3.nip5;1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152615/
https://www.ncbi.nlm.nih.gov/pubmed/31705705
http://dx.doi.org/10.1111/pbi.13288
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