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Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial

Nanocomposite scaffolds combining carbon nanomaterials (CNMs) with a biocompatible matrix are able to favor the neuronal differentiation and growth of a number of cell types, because they mimic neural-tissue nanotopography and/or conductivity. We performed comparative analysis of biomimetic scaffold...

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Autores principales: Tonellato, Marika, Piccione, Monica, Gasparotto, Matteo, Bellet, Pietro, Tibaudo, Lucia, Vicentini, Nicola, Bergantino, Elisabetta, Menna, Enzo, Vitiello, Libero, Di Liddo, Rosa, Filippini, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152835/
https://www.ncbi.nlm.nih.gov/pubmed/32120984
http://dx.doi.org/10.3390/nano10030415
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author Tonellato, Marika
Piccione, Monica
Gasparotto, Matteo
Bellet, Pietro
Tibaudo, Lucia
Vicentini, Nicola
Bergantino, Elisabetta
Menna, Enzo
Vitiello, Libero
Di Liddo, Rosa
Filippini, Francesco
author_facet Tonellato, Marika
Piccione, Monica
Gasparotto, Matteo
Bellet, Pietro
Tibaudo, Lucia
Vicentini, Nicola
Bergantino, Elisabetta
Menna, Enzo
Vitiello, Libero
Di Liddo, Rosa
Filippini, Francesco
author_sort Tonellato, Marika
collection PubMed
description Nanocomposite scaffolds combining carbon nanomaterials (CNMs) with a biocompatible matrix are able to favor the neuronal differentiation and growth of a number of cell types, because they mimic neural-tissue nanotopography and/or conductivity. We performed comparative analysis of biomimetic scaffolds with poly-L-lactic acid (PLLA) matrix and three different p-methoxyphenyl functionalized carbon nanofillers, namely, carbon nanotubes (CNTs), carbon nanohorns (CNHs), and reduced graphene oxide (RGO), dispersed at varying concentrations. qRT-PCR analysis of the modulation of neuronal markers in human circulating multipotent cells cultured on nanocomposite scaffolds showed high variability in their expression patterns depending on the scaffolds’ inhomogeneities. Local stimuli variation could result in a multi- to oligopotency shift and commitment towards multiple cell lineages, which was assessed by the qRT-PCR profiling of markers for neural, adipogenic, and myogenic cell lineages. Less conductive scaffolds, i.e., bare poly-L-lactic acid (PLLA)-, CNH-, and RGO-based nanocomposites, appeared to boost the expression of myogenic-lineage marker genes. Moreover, scaffolds are much more effective on early commitment than in subsequent differentiation. This work suggests that biomimetic PLLA carbon-nanomaterial (PLLA-CNM) scaffolds combined with multipotent autologous cells can represent a powerful tool in the regenerative medicine of multiple tissue types, opening the route to next analyses with specific and standardized scaffold features.
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spelling pubmed-71528352020-04-20 Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial Tonellato, Marika Piccione, Monica Gasparotto, Matteo Bellet, Pietro Tibaudo, Lucia Vicentini, Nicola Bergantino, Elisabetta Menna, Enzo Vitiello, Libero Di Liddo, Rosa Filippini, Francesco Nanomaterials (Basel) Article Nanocomposite scaffolds combining carbon nanomaterials (CNMs) with a biocompatible matrix are able to favor the neuronal differentiation and growth of a number of cell types, because they mimic neural-tissue nanotopography and/or conductivity. We performed comparative analysis of biomimetic scaffolds with poly-L-lactic acid (PLLA) matrix and three different p-methoxyphenyl functionalized carbon nanofillers, namely, carbon nanotubes (CNTs), carbon nanohorns (CNHs), and reduced graphene oxide (RGO), dispersed at varying concentrations. qRT-PCR analysis of the modulation of neuronal markers in human circulating multipotent cells cultured on nanocomposite scaffolds showed high variability in their expression patterns depending on the scaffolds’ inhomogeneities. Local stimuli variation could result in a multi- to oligopotency shift and commitment towards multiple cell lineages, which was assessed by the qRT-PCR profiling of markers for neural, adipogenic, and myogenic cell lineages. Less conductive scaffolds, i.e., bare poly-L-lactic acid (PLLA)-, CNH-, and RGO-based nanocomposites, appeared to boost the expression of myogenic-lineage marker genes. Moreover, scaffolds are much more effective on early commitment than in subsequent differentiation. This work suggests that biomimetic PLLA carbon-nanomaterial (PLLA-CNM) scaffolds combined with multipotent autologous cells can represent a powerful tool in the regenerative medicine of multiple tissue types, opening the route to next analyses with specific and standardized scaffold features. MDPI 2020-02-27 /pmc/articles/PMC7152835/ /pubmed/32120984 http://dx.doi.org/10.3390/nano10030415 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tonellato, Marika
Piccione, Monica
Gasparotto, Matteo
Bellet, Pietro
Tibaudo, Lucia
Vicentini, Nicola
Bergantino, Elisabetta
Menna, Enzo
Vitiello, Libero
Di Liddo, Rosa
Filippini, Francesco
Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title_full Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title_fullStr Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title_full_unstemmed Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title_short Commitment of Autologous Human Multipotent Stem Cells on Biomimetic Poly-L-Lactic Acid-Based Scaffolds Is Strongly Influenced by Structure and Concentration of Carbon Nanomaterial
title_sort commitment of autologous human multipotent stem cells on biomimetic poly-l-lactic acid-based scaffolds is strongly influenced by structure and concentration of carbon nanomaterial
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152835/
https://www.ncbi.nlm.nih.gov/pubmed/32120984
http://dx.doi.org/10.3390/nano10030415
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