Cargando…

Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization

Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of diff...

Descripción completa

Detalles Bibliográficos
Autores principales: Khan, Abdullah O., Di Maio, Alessandro, Guggenheim, Emily J., Chetwynd, Andrew J., Pencross, Dan, Tang, Selina, Belinga-Desaunay, Marie-France A., Thomas, Steven G., Rappoport, Joshua Z., Lynch, Iseult
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152854/
https://www.ncbi.nlm.nih.gov/pubmed/32106393
http://dx.doi.org/10.3390/nano10030401
Descripción
Sumario:Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO(2) NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO(2) NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO(2) NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces.