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Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization
Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of diff...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152854/ https://www.ncbi.nlm.nih.gov/pubmed/32106393 http://dx.doi.org/10.3390/nano10030401 |
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author | Khan, Abdullah O. Di Maio, Alessandro Guggenheim, Emily J. Chetwynd, Andrew J. Pencross, Dan Tang, Selina Belinga-Desaunay, Marie-France A. Thomas, Steven G. Rappoport, Joshua Z. Lynch, Iseult |
author_facet | Khan, Abdullah O. Di Maio, Alessandro Guggenheim, Emily J. Chetwynd, Andrew J. Pencross, Dan Tang, Selina Belinga-Desaunay, Marie-France A. Thomas, Steven G. Rappoport, Joshua Z. Lynch, Iseult |
author_sort | Khan, Abdullah O. |
collection | PubMed |
description | Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO(2) NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO(2) NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO(2) NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces. |
format | Online Article Text |
id | pubmed-7152854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71528542020-04-20 Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization Khan, Abdullah O. Di Maio, Alessandro Guggenheim, Emily J. Chetwynd, Andrew J. Pencross, Dan Tang, Selina Belinga-Desaunay, Marie-France A. Thomas, Steven G. Rappoport, Joshua Z. Lynch, Iseult Nanomaterials (Basel) Article Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO(2) NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO(2) NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO(2) NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces. MDPI 2020-02-25 /pmc/articles/PMC7152854/ /pubmed/32106393 http://dx.doi.org/10.3390/nano10030401 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khan, Abdullah O. Di Maio, Alessandro Guggenheim, Emily J. Chetwynd, Andrew J. Pencross, Dan Tang, Selina Belinga-Desaunay, Marie-France A. Thomas, Steven G. Rappoport, Joshua Z. Lynch, Iseult Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title_full | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title_fullStr | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title_full_unstemmed | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title_short | Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO(2) Nanomaterials Following Uptake and Sub-Cellular Localization |
title_sort | surface chemistry-dependent evolution of the nanomaterial corona on tio(2) nanomaterials following uptake and sub-cellular localization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7152854/ https://www.ncbi.nlm.nih.gov/pubmed/32106393 http://dx.doi.org/10.3390/nano10030401 |
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