Cargando…

Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma

OBJECTIVE: Overexpression of microRNA-21 (miR-21) increases the radiation resistance of esophageal squamous cell carcinoma (ESCC). However, the molecular mechanism responsible for this action is still unclear. In the present study, we investigated the role of phosphatase and tensin homolog deleted o...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, Jun, Lv, Yinxiang, Wu, Chaochao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153193/
https://www.ncbi.nlm.nih.gov/pubmed/32268810
http://dx.doi.org/10.1177/0300060519882543
_version_ 1783521607881326592
author Peng, Jun
Lv, Yinxiang
Wu, Chaochao
author_facet Peng, Jun
Lv, Yinxiang
Wu, Chaochao
author_sort Peng, Jun
collection PubMed
description OBJECTIVE: Overexpression of microRNA-21 (miR-21) increases the radiation resistance of esophageal squamous cell carcinoma (ESCC). However, the molecular mechanism responsible for this action is still unclear. In the present study, we investigated the role of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in miR-21-enhanced radiation resistance in patients with ESCC. METHODS: We evaluated the association between miR-21 levels and radiation resistance in patients with ESCC. We also investigated the role of PTEN in the proliferation and apoptosis of ESCC cells transfected with miR-21 inhibitor during irradiation, using PTEN small interfering RNA (siRNA). RESULTS: MiR-21 levels were significantly higher in radiation-resistant patients. Downregulation of miR-21 during irradiation suppressed the radiation resistance of ESCC cells, demonstrated by decreased cell proliferation and increased cell apoptosis. PTEN siRNA attenuated miR-21-induced suppression of radiation resistance in ESCC cells. CONCLUSIONS: These results suggest that miR-21 enhanced the radiation resistance of ESCC by inhibiting PTEN. MiR-21 and PTEN are potential therapeutic biotargets for ESCC.
format Online
Article
Text
id pubmed-7153193
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-71531932020-04-20 Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma Peng, Jun Lv, Yinxiang Wu, Chaochao J Int Med Res Prospective Clinical Research Report OBJECTIVE: Overexpression of microRNA-21 (miR-21) increases the radiation resistance of esophageal squamous cell carcinoma (ESCC). However, the molecular mechanism responsible for this action is still unclear. In the present study, we investigated the role of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in miR-21-enhanced radiation resistance in patients with ESCC. METHODS: We evaluated the association between miR-21 levels and radiation resistance in patients with ESCC. We also investigated the role of PTEN in the proliferation and apoptosis of ESCC cells transfected with miR-21 inhibitor during irradiation, using PTEN small interfering RNA (siRNA). RESULTS: MiR-21 levels were significantly higher in radiation-resistant patients. Downregulation of miR-21 during irradiation suppressed the radiation resistance of ESCC cells, demonstrated by decreased cell proliferation and increased cell apoptosis. PTEN siRNA attenuated miR-21-induced suppression of radiation resistance in ESCC cells. CONCLUSIONS: These results suggest that miR-21 enhanced the radiation resistance of ESCC by inhibiting PTEN. MiR-21 and PTEN are potential therapeutic biotargets for ESCC. SAGE Publications 2020-04-08 /pmc/articles/PMC7153193/ /pubmed/32268810 http://dx.doi.org/10.1177/0300060519882543 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Prospective Clinical Research Report
Peng, Jun
Lv, Yinxiang
Wu, Chaochao
Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title_full Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title_fullStr Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title_full_unstemmed Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title_short Radiation-resistance increased by overexpression of microRNA-21 and inhibition of its target PTEN in esophageal squamous cell carcinoma
title_sort radiation-resistance increased by overexpression of microrna-21 and inhibition of its target pten in esophageal squamous cell carcinoma
topic Prospective Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153193/
https://www.ncbi.nlm.nih.gov/pubmed/32268810
http://dx.doi.org/10.1177/0300060519882543
work_keys_str_mv AT pengjun radiationresistanceincreasedbyoverexpressionofmicrorna21andinhibitionofitstargetpteninesophagealsquamouscellcarcinoma
AT lvyinxiang radiationresistanceincreasedbyoverexpressionofmicrorna21andinhibitionofitstargetpteninesophagealsquamouscellcarcinoma
AT wuchaochao radiationresistanceincreasedbyoverexpressionofmicrorna21andinhibitionofitstargetpteninesophagealsquamouscellcarcinoma