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Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries

The metabolic activity in plants or fruits is associated with volatile organic compounds (VOCs), which can help identify the different diseases. P-ethylphenol has been demonstrated as one of the most important VOCs released by the Phytophthora cactorum (P. cactorum) infected strawberries. In this st...

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Autores principales: Wang, Hui, Wang, Yue, Hou, Xiaopeng, Xiong, Benhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153259/
https://www.ncbi.nlm.nih.gov/pubmed/32155991
http://dx.doi.org/10.3390/nano10030479
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author Wang, Hui
Wang, Yue
Hou, Xiaopeng
Xiong, Benhai
author_facet Wang, Hui
Wang, Yue
Hou, Xiaopeng
Xiong, Benhai
author_sort Wang, Hui
collection PubMed
description The metabolic activity in plants or fruits is associated with volatile organic compounds (VOCs), which can help identify the different diseases. P-ethylphenol has been demonstrated as one of the most important VOCs released by the Phytophthora cactorum (P. cactorum) infected strawberries. In this study, a bioelectronic nose based on a gas biosensor array and signal processing model was developed for the noninvasive diagnostics of the P. cactorum infected strawberries, which could overcome the limitations of the traditional spectral analysis methods. The gas biosensor array was fabricated using the single-wall carbon nanotubes (SWNTs) immobilized on the surface of field-effect transistor, and then non-covalently functionalized with different single-strand DNAs (ssDNA) through π–π interaction. The characteristics of ssDNA-SWNTs were investigated using scanning electron microscope, atomic force microscopy, Raman, UV spectroscopy, and electrical measurements, indicating that ssDNA-SWNTs revealed excellent stability and repeatability. By comparing the responses of different ssDNA-SWNTs, the sensitivity to P-ethylphenol was significantly higher for the s6DNA-SWNTs than other ssDNA-SWNTs, in which the limit of detection reached 0.13% saturated vapor of P-ethylphenol. However, s6DNA-SWNTs can still be interfered with by other VOCs emitted by the strawberries in the view of poor selectivity. The bioelectronic nose took advantage of the different sensitivities of different gas biosensors to different VOCs. To improve measure precision, all ssDNA-SWNTs as a gas biosensor array were applied to monitor the different VOCs released by the strawberries, and the detecting data were processed by neural network fitting (NNF) and Gaussian process regression (GPR) with high accuracy.
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spelling pubmed-71532592020-04-20 Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries Wang, Hui Wang, Yue Hou, Xiaopeng Xiong, Benhai Nanomaterials (Basel) Article The metabolic activity in plants or fruits is associated with volatile organic compounds (VOCs), which can help identify the different diseases. P-ethylphenol has been demonstrated as one of the most important VOCs released by the Phytophthora cactorum (P. cactorum) infected strawberries. In this study, a bioelectronic nose based on a gas biosensor array and signal processing model was developed for the noninvasive diagnostics of the P. cactorum infected strawberries, which could overcome the limitations of the traditional spectral analysis methods. The gas biosensor array was fabricated using the single-wall carbon nanotubes (SWNTs) immobilized on the surface of field-effect transistor, and then non-covalently functionalized with different single-strand DNAs (ssDNA) through π–π interaction. The characteristics of ssDNA-SWNTs were investigated using scanning electron microscope, atomic force microscopy, Raman, UV spectroscopy, and electrical measurements, indicating that ssDNA-SWNTs revealed excellent stability and repeatability. By comparing the responses of different ssDNA-SWNTs, the sensitivity to P-ethylphenol was significantly higher for the s6DNA-SWNTs than other ssDNA-SWNTs, in which the limit of detection reached 0.13% saturated vapor of P-ethylphenol. However, s6DNA-SWNTs can still be interfered with by other VOCs emitted by the strawberries in the view of poor selectivity. The bioelectronic nose took advantage of the different sensitivities of different gas biosensors to different VOCs. To improve measure precision, all ssDNA-SWNTs as a gas biosensor array were applied to monitor the different VOCs released by the strawberries, and the detecting data were processed by neural network fitting (NNF) and Gaussian process regression (GPR) with high accuracy. MDPI 2020-03-07 /pmc/articles/PMC7153259/ /pubmed/32155991 http://dx.doi.org/10.3390/nano10030479 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Hui
Wang, Yue
Hou, Xiaopeng
Xiong, Benhai
Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title_full Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title_fullStr Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title_full_unstemmed Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title_short Bioelectronic Nose Based on Single-Stranded DNA and Single-Walled Carbon Nanotube to Identify a Major Plant Volatile Organic Compound (p-Ethylphenol) Released by Phytophthora Cactorum Infected Strawberries
title_sort bioelectronic nose based on single-stranded dna and single-walled carbon nanotube to identify a major plant volatile organic compound (p-ethylphenol) released by phytophthora cactorum infected strawberries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153259/
https://www.ncbi.nlm.nih.gov/pubmed/32155991
http://dx.doi.org/10.3390/nano10030479
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