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Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake

Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purifi...

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Autores principales: Ramírez-Jiménez, Rafael, Artiga, Álvaro, Mitchell, Scott G., Martín-Rapún, Rafael, de la Fuente, Jesús M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153367/
https://www.ncbi.nlm.nih.gov/pubmed/32192152
http://dx.doi.org/10.3390/nano10030539
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author Ramírez-Jiménez, Rafael
Artiga, Álvaro
Mitchell, Scott G.
Martín-Rapún, Rafael
de la Fuente, Jesús M.
author_facet Ramírez-Jiménez, Rafael
Artiga, Álvaro
Mitchell, Scott G.
Martín-Rapún, Rafael
de la Fuente, Jesús M.
author_sort Ramírez-Jiménez, Rafael
collection PubMed
description Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications.
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spelling pubmed-71533672020-04-20 Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake Ramírez-Jiménez, Rafael Artiga, Álvaro Mitchell, Scott G. Martín-Rapún, Rafael de la Fuente, Jesús M. Nanomaterials (Basel) Article Gold nanoprisms possess remarkable optical properties that make them useful for medical biotechnology applications such as diagnosis and photothermal therapy. However, shape-selective synthesis of gold nanoprisms is not trivial and typically requires either toxic surfactants or time-consuming purification protocols, which can limit their applicability. Here, we show how triangular gold nanoprisms of different sizes can be purified by precipitation using the non-toxic glutathione ligand, thereby removing the need for toxic surfactants and bottleneck purification techniques. The protocol is amenable for direct scaling up as no instrumentation is required in the critical purification step. The new purification method provides a two-fold increased yield in gold nanoprisms compared to electrophoretic filtration, while providing nanoprisms of similar localized surface plasmon resonance wavelength. Crucially, the gold nanoprisms isolated using this methodology show fewer non-specific interactions with cells and lower cellular internalization, which paves the way for a higher selectivity in therapeutic applications. MDPI 2020-03-17 /pmc/articles/PMC7153367/ /pubmed/32192152 http://dx.doi.org/10.3390/nano10030539 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramírez-Jiménez, Rafael
Artiga, Álvaro
Mitchell, Scott G.
Martín-Rapún, Rafael
de la Fuente, Jesús M.
Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title_full Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title_fullStr Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title_full_unstemmed Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title_short Surfactant-Free Synthesis and Scalable Purification of Triangular Gold Nanoprisms with Low Non-Specific Cellular Uptake
title_sort surfactant-free synthesis and scalable purification of triangular gold nanoprisms with low non-specific cellular uptake
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153367/
https://www.ncbi.nlm.nih.gov/pubmed/32192152
http://dx.doi.org/10.3390/nano10030539
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