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Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known abou...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153391/ https://www.ncbi.nlm.nih.gov/pubmed/32204428 http://dx.doi.org/10.3390/nano10030553 |
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author | Claveau, Sandra Nehlig, Émilie Garcia-Argote, Sébastien Feuillastre, Sophie Pieters, Grégory Girard, Hugues A. Arnault, Jean-Charles Treussart, François Bertrand, Jean-Rémi |
author_facet | Claveau, Sandra Nehlig, Émilie Garcia-Argote, Sébastien Feuillastre, Sophie Pieters, Grégory Girard, Hugues A. Arnault, Jean-Charles Treussart, François Bertrand, Jean-Rémi |
author_sort | Claveau, Sandra |
collection | PubMed |
description | Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. |
format | Online Article Text |
id | pubmed-7153391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71533912020-04-20 Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution Claveau, Sandra Nehlig, Émilie Garcia-Argote, Sébastien Feuillastre, Sophie Pieters, Grégory Girard, Hugues A. Arnault, Jean-Charles Treussart, François Bertrand, Jean-Rémi Nanomaterials (Basel) Article Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. MDPI 2020-03-19 /pmc/articles/PMC7153391/ /pubmed/32204428 http://dx.doi.org/10.3390/nano10030553 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Claveau, Sandra Nehlig, Émilie Garcia-Argote, Sébastien Feuillastre, Sophie Pieters, Grégory Girard, Hugues A. Arnault, Jean-Charles Treussart, François Bertrand, Jean-Rémi Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title_full | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title_fullStr | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title_full_unstemmed | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title_short | Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution |
title_sort | delivery of sirna to ewing sarcoma tumor xenografted on mice, using hydrogenated detonation nanodiamonds: treatment efficacy and tissue distribution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153391/ https://www.ncbi.nlm.nih.gov/pubmed/32204428 http://dx.doi.org/10.3390/nano10030553 |
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