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Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution

Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known abou...

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Autores principales: Claveau, Sandra, Nehlig, Émilie, Garcia-Argote, Sébastien, Feuillastre, Sophie, Pieters, Grégory, Girard, Hugues A., Arnault, Jean-Charles, Treussart, François, Bertrand, Jean-Rémi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153391/
https://www.ncbi.nlm.nih.gov/pubmed/32204428
http://dx.doi.org/10.3390/nano10030553
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author Claveau, Sandra
Nehlig, Émilie
Garcia-Argote, Sébastien
Feuillastre, Sophie
Pieters, Grégory
Girard, Hugues A.
Arnault, Jean-Charles
Treussart, François
Bertrand, Jean-Rémi
author_facet Claveau, Sandra
Nehlig, Émilie
Garcia-Argote, Sébastien
Feuillastre, Sophie
Pieters, Grégory
Girard, Hugues A.
Arnault, Jean-Charles
Treussart, François
Bertrand, Jean-Rémi
author_sort Claveau, Sandra
collection PubMed
description Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA.
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spelling pubmed-71533912020-04-20 Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution Claveau, Sandra Nehlig, Émilie Garcia-Argote, Sébastien Feuillastre, Sophie Pieters, Grégory Girard, Hugues A. Arnault, Jean-Charles Treussart, François Bertrand, Jean-Rémi Nanomaterials (Basel) Article Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and efficacy of nanodiamonds-based therapy in mice. In this report, we studied the capacity of cationic hydrogenated detonation nanodiamonds to carry active small interfering RNA (siRNA) in a mice model of Ewing sarcoma, a bone cancer of young adults due in the vast majority to the EWS-FLI1 junction oncogene. Replacing hydrogen gas by its radioactive analog tritium gas led to the formation of labeled nanodiamonds and allowed us to investigate their distribution throughout mouse organs and their excretion in urine and feces. We also demonstrated that siRNA directed against EWS-FLI1 inhibited this oncogene expression in tumor xenografted on mice. This work is a significant step to establish cationic hydrogenated detonation nanodiamond as an effective agent for in vivo delivery of active siRNA. MDPI 2020-03-19 /pmc/articles/PMC7153391/ /pubmed/32204428 http://dx.doi.org/10.3390/nano10030553 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Claveau, Sandra
Nehlig, Émilie
Garcia-Argote, Sébastien
Feuillastre, Sophie
Pieters, Grégory
Girard, Hugues A.
Arnault, Jean-Charles
Treussart, François
Bertrand, Jean-Rémi
Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title_full Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title_fullStr Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title_full_unstemmed Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title_short Delivery of siRNA to Ewing Sarcoma Tumor Xenografted on Mice, Using Hydrogenated Detonation Nanodiamonds: Treatment Efficacy and Tissue Distribution
title_sort delivery of sirna to ewing sarcoma tumor xenografted on mice, using hydrogenated detonation nanodiamonds: treatment efficacy and tissue distribution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153391/
https://www.ncbi.nlm.nih.gov/pubmed/32204428
http://dx.doi.org/10.3390/nano10030553
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