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Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening
Biocompatibility is very important for cell growth using 3D printers, but biocompatibility materials are very expensive. In this study, we investigated the possibility of cell culture by the surface modification of relatively low-cost industrial materials and an efficient three-dimensional (3D) scaf...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153510/ https://www.ncbi.nlm.nih.gov/pubmed/32183472 http://dx.doi.org/10.3390/nano10030529 |
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author | N’deh, Kaudjhis Patrick Ulrich Kim, Gyeong-Ji Chung, Kang-Hyun Shin, Jae-Soo Lee, Kwang-Sup Choi, Jeong-Woo Lee, Kwon-Jai An, Jeung Hee |
author_facet | N’deh, Kaudjhis Patrick Ulrich Kim, Gyeong-Ji Chung, Kang-Hyun Shin, Jae-Soo Lee, Kwang-Sup Choi, Jeong-Woo Lee, Kwon-Jai An, Jeung Hee |
author_sort | N’deh, Kaudjhis Patrick Ulrich |
collection | PubMed |
description | Biocompatibility is very important for cell growth using 3D printers, but biocompatibility materials are very expensive. In this study, we investigated the possibility of cell culture by the surface modification of relatively low-cost industrial materials and an efficient three-dimensional (3D) scaffold made with an industrial ABS filament for cell proliferation, spheroid formation, and drug screening applications. We evaluated the adequate structure among two-layer square shape 3D scaffolds printed by fused deposition modeling with variable infill densities (10–50%). Based on the effects of these scaffolds on cell proliferation and spheroid formation, we conducted experiments using the industrial ABS 3D scaffold (IA3D) with 40% of infill density, which presented an external dimension of (XYZ) 7650 µm × 7647 µm × 210 µm, 29.8% porosity, and 225 homogenous micropores (251.6 µm × 245.9 µm × 210 µm). In the IA3D, spheroids of cancer HepG2 cells and keratinocytes HaCaT cells appeared after 2 and 3 days of culture, respectively, whereas no spheroids were formed in 2D culture. A gold nanoparticle-coated industrial ABS 3D scaffold (GIA3D) exhibited enhanced biocompatible properties including increased spheroid formation by HepG2 cells compared to IA3D (1.3-fold) and 2D (38-fold) cultures. Furthermore, the cancer cells exhibited increased resistance to drug treatments in GIA3D, with cell viabilities of 122.9% in industrial GIA3D, 40.2% in IA3D, and 55.2% in 2D cultures when treated with 100 µM of mitoxantrone. Our results show that the newly engineered IA3D is an innovative 3D scaffold with upgraded properties for cell proliferation, spheroid formation, and drug-screening applications. |
format | Online Article Text |
id | pubmed-7153510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71535102020-04-20 Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening N’deh, Kaudjhis Patrick Ulrich Kim, Gyeong-Ji Chung, Kang-Hyun Shin, Jae-Soo Lee, Kwang-Sup Choi, Jeong-Woo Lee, Kwon-Jai An, Jeung Hee Nanomaterials (Basel) Article Biocompatibility is very important for cell growth using 3D printers, but biocompatibility materials are very expensive. In this study, we investigated the possibility of cell culture by the surface modification of relatively low-cost industrial materials and an efficient three-dimensional (3D) scaffold made with an industrial ABS filament for cell proliferation, spheroid formation, and drug screening applications. We evaluated the adequate structure among two-layer square shape 3D scaffolds printed by fused deposition modeling with variable infill densities (10–50%). Based on the effects of these scaffolds on cell proliferation and spheroid formation, we conducted experiments using the industrial ABS 3D scaffold (IA3D) with 40% of infill density, which presented an external dimension of (XYZ) 7650 µm × 7647 µm × 210 µm, 29.8% porosity, and 225 homogenous micropores (251.6 µm × 245.9 µm × 210 µm). In the IA3D, spheroids of cancer HepG2 cells and keratinocytes HaCaT cells appeared after 2 and 3 days of culture, respectively, whereas no spheroids were formed in 2D culture. A gold nanoparticle-coated industrial ABS 3D scaffold (GIA3D) exhibited enhanced biocompatible properties including increased spheroid formation by HepG2 cells compared to IA3D (1.3-fold) and 2D (38-fold) cultures. Furthermore, the cancer cells exhibited increased resistance to drug treatments in GIA3D, with cell viabilities of 122.9% in industrial GIA3D, 40.2% in IA3D, and 55.2% in 2D cultures when treated with 100 µM of mitoxantrone. Our results show that the newly engineered IA3D is an innovative 3D scaffold with upgraded properties for cell proliferation, spheroid formation, and drug-screening applications. MDPI 2020-03-15 /pmc/articles/PMC7153510/ /pubmed/32183472 http://dx.doi.org/10.3390/nano10030529 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article N’deh, Kaudjhis Patrick Ulrich Kim, Gyeong-Ji Chung, Kang-Hyun Shin, Jae-Soo Lee, Kwang-Sup Choi, Jeong-Woo Lee, Kwon-Jai An, Jeung Hee Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title | Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title_full | Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title_fullStr | Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title_full_unstemmed | Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title_short | Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening |
title_sort | surface-modified industrial acrylonitrile butadiene styrene 3d scaffold fabrication by gold nanoparticle for drug screening |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153510/ https://www.ncbi.nlm.nih.gov/pubmed/32183472 http://dx.doi.org/10.3390/nano10030529 |
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