Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate
Most of the research efforts involving the bovine gastrointestinal microbiota have focused on cattle’s forestomach, particularly the rumen, so information concerning the bovine fecal microbiota is more scarce, especially in young beef cattle. The present study was performed to evaluate the ruminal a...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153887/ https://www.ncbi.nlm.nih.gov/pubmed/32282837 http://dx.doi.org/10.1371/journal.pone.0231533 |
_version_ | 1783521728852393984 |
---|---|
author | Lourenco, Jeferson M. Kieran, Troy J. Seidel, Darren S. Glenn, Travis C. da Silveira, Magali F. Callaway, Todd R. Stewart, R. Lawton |
author_facet | Lourenco, Jeferson M. Kieran, Troy J. Seidel, Darren S. Glenn, Travis C. da Silveira, Magali F. Callaway, Todd R. Stewart, R. Lawton |
author_sort | Lourenco, Jeferson M. |
collection | PubMed |
description | Most of the research efforts involving the bovine gastrointestinal microbiota have focused on cattle’s forestomach, particularly the rumen, so information concerning the bovine fecal microbiota is more scarce, especially in young beef cattle. The present study was performed to evaluate the ruminal and fecal microbiotas of beef calves as they reached the end of their nursing phase. A total of 18 Angus cow/calf pairs were selected and assigned to one of two treatment groups for the last 92 days of the calves’ nursing period, as follows: 1) calves were supplemented with concentrate in a creep feeding system; or 2) control group with no supplementation of calves. After 92 days, ruminal and fecal samples were individually obtained from calves in both groups, and their microbiotas were evaluated using 16S rRNA gene sequencing. Ruminal samples were predominated by Prevotella (18 to 23% of the total bacterial abundance), regardless if calves received supplementation or not; however, in the feces, Prevotella was only the seventh most abundant genus (0.6 to 2.1% of total bacterial abundance). Both the rumen (P = 0.01) and the feces (P = 0.05) of calves that received supplementation had greater abundance of Firmicutes. In addition, calves that were supplemented had lower abundance of Fibrobacteres (P = 0.03) in their rumens. Regardless if the calves were supplemented or not, Faith’s Phylogenetic Diversity index (P ≤ 0.007) and total concentration of short chain fatty acids (P < 0.001) were both greater in the rumen than in the feces of calves. In summary, the ruminal and fecal microbiotas of weanling beef calves were considerably distinct. Additionally, supplementation with creep feed caused some significant changes in the composition of the gastrointestinal microbiota of the calves, especially in the rumen, where supplementation caused an increase in Firmicutes and a decrease in abundance of Fibrobacteres. |
format | Online Article Text |
id | pubmed-7153887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-71538872020-04-16 Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate Lourenco, Jeferson M. Kieran, Troy J. Seidel, Darren S. Glenn, Travis C. da Silveira, Magali F. Callaway, Todd R. Stewart, R. Lawton PLoS One Research Article Most of the research efforts involving the bovine gastrointestinal microbiota have focused on cattle’s forestomach, particularly the rumen, so information concerning the bovine fecal microbiota is more scarce, especially in young beef cattle. The present study was performed to evaluate the ruminal and fecal microbiotas of beef calves as they reached the end of their nursing phase. A total of 18 Angus cow/calf pairs were selected and assigned to one of two treatment groups for the last 92 days of the calves’ nursing period, as follows: 1) calves were supplemented with concentrate in a creep feeding system; or 2) control group with no supplementation of calves. After 92 days, ruminal and fecal samples were individually obtained from calves in both groups, and their microbiotas were evaluated using 16S rRNA gene sequencing. Ruminal samples were predominated by Prevotella (18 to 23% of the total bacterial abundance), regardless if calves received supplementation or not; however, in the feces, Prevotella was only the seventh most abundant genus (0.6 to 2.1% of total bacterial abundance). Both the rumen (P = 0.01) and the feces (P = 0.05) of calves that received supplementation had greater abundance of Firmicutes. In addition, calves that were supplemented had lower abundance of Fibrobacteres (P = 0.03) in their rumens. Regardless if the calves were supplemented or not, Faith’s Phylogenetic Diversity index (P ≤ 0.007) and total concentration of short chain fatty acids (P < 0.001) were both greater in the rumen than in the feces of calves. In summary, the ruminal and fecal microbiotas of weanling beef calves were considerably distinct. Additionally, supplementation with creep feed caused some significant changes in the composition of the gastrointestinal microbiota of the calves, especially in the rumen, where supplementation caused an increase in Firmicutes and a decrease in abundance of Fibrobacteres. Public Library of Science 2020-04-13 /pmc/articles/PMC7153887/ /pubmed/32282837 http://dx.doi.org/10.1371/journal.pone.0231533 Text en © 2020 Lourenco et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lourenco, Jeferson M. Kieran, Troy J. Seidel, Darren S. Glenn, Travis C. da Silveira, Magali F. Callaway, Todd R. Stewart, R. Lawton Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title | Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title_full | Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title_fullStr | Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title_full_unstemmed | Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title_short | Comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
title_sort | comparison of the ruminal and fecal microbiotas in beef calves supplemented or not with concentrate |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7153887/ https://www.ncbi.nlm.nih.gov/pubmed/32282837 http://dx.doi.org/10.1371/journal.pone.0231533 |
work_keys_str_mv | AT lourencojefersonm comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT kierantroyj comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT seideldarrens comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT glenntravisc comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT dasilveiramagalif comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT callawaytoddr comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate AT stewartrlawton comparisonoftheruminalandfecalmicrobiotasinbeefcalvessupplementedornotwithconcentrate |