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Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results
OBJECTIVE: In this study, in order to investigate the usefulness of intratracheal instillation in assessing the pulmonary toxicity of nanomaterials, intratracheal instillation of nickel oxide‐nanoparticles (NiO‐NP) was performed. METHODS: In this study, rats were administered test materials by intra...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154589/ https://www.ncbi.nlm.nih.gov/pubmed/32515878 http://dx.doi.org/10.1002/1348-9585.12117 |
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author | Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Fukushima, Shoji Oshima, Yutaka Kobayashi, Toshio Morimoto, Yasuo Izumi, Hiroto Ota, Yasufumi Takehara, Hiroshi Numano, Takamasa Kawabe, Mayumi Gamo, Masashi Takeshita, Jun‐ichi |
author_facet | Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Fukushima, Shoji Oshima, Yutaka Kobayashi, Toshio Morimoto, Yasuo Izumi, Hiroto Ota, Yasufumi Takehara, Hiroshi Numano, Takamasa Kawabe, Mayumi Gamo, Masashi Takeshita, Jun‐ichi |
author_sort | Senoh, Hideki |
collection | PubMed |
description | OBJECTIVE: In this study, in order to investigate the usefulness of intratracheal instillation in assessing the pulmonary toxicity of nanomaterials, intratracheal instillation of nickel oxide‐nanoparticles (NiO‐NP) was performed. METHODS: In this study, rats were administered test materials by intratracheal instillation at five different research institutions in order to assess the validity of using intratracheal instillation for hazard identification of nanomaterials. Eight‐week‐old male SD rats were administered NiO‐NP dispersed in deionized water by a single intratracheal instillation at doses of 0 (vehicle control), 0.2, 0.67, and 2 mg/kg BW. Three days after instillation, histopathological examination of the lungs was performed. RESULTS: NiO‐NP was distributed in the vicinity of hilus of the lung and in the alveoli around the bronchioles. Histopathological changes such as degeneration/necrosis of macrophages, inflammation, and proliferation of type II pneumocyte in the lung were observed, and their severity corresponded with increasing dose. The histopathological observations of pulmonary toxicity were almost similar at each institution. CONCLUSION: The similarity of the histopathological changes observed by five independent groups indicates that intratracheal instillation can be a useful screening method to detect the pulmonary toxicity of nanomaterials. |
format | Online Article Text |
id | pubmed-7154589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71545892020-04-15 Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Fukushima, Shoji Oshima, Yutaka Kobayashi, Toshio Morimoto, Yasuo Izumi, Hiroto Ota, Yasufumi Takehara, Hiroshi Numano, Takamasa Kawabe, Mayumi Gamo, Masashi Takeshita, Jun‐ichi J Occup Health Brief Reports OBJECTIVE: In this study, in order to investigate the usefulness of intratracheal instillation in assessing the pulmonary toxicity of nanomaterials, intratracheal instillation of nickel oxide‐nanoparticles (NiO‐NP) was performed. METHODS: In this study, rats were administered test materials by intratracheal instillation at five different research institutions in order to assess the validity of using intratracheal instillation for hazard identification of nanomaterials. Eight‐week‐old male SD rats were administered NiO‐NP dispersed in deionized water by a single intratracheal instillation at doses of 0 (vehicle control), 0.2, 0.67, and 2 mg/kg BW. Three days after instillation, histopathological examination of the lungs was performed. RESULTS: NiO‐NP was distributed in the vicinity of hilus of the lung and in the alveoli around the bronchioles. Histopathological changes such as degeneration/necrosis of macrophages, inflammation, and proliferation of type II pneumocyte in the lung were observed, and their severity corresponded with increasing dose. The histopathological observations of pulmonary toxicity were almost similar at each institution. CONCLUSION: The similarity of the histopathological changes observed by five independent groups indicates that intratracheal instillation can be a useful screening method to detect the pulmonary toxicity of nanomaterials. John Wiley and Sons Inc. 2020-03-10 /pmc/articles/PMC7154589/ /pubmed/32515878 http://dx.doi.org/10.1002/1348-9585.12117 Text en © 2020 The Authors. Journal of Occupational Health published by John Wiley & Sons Australia, Ltd on behalf of The Japan Society for Occupational Health This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Brief Reports Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Fukushima, Shoji Oshima, Yutaka Kobayashi, Toshio Morimoto, Yasuo Izumi, Hiroto Ota, Yasufumi Takehara, Hiroshi Numano, Takamasa Kawabe, Mayumi Gamo, Masashi Takeshita, Jun‐ichi Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title | Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title_full | Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title_fullStr | Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title_full_unstemmed | Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title_short | Inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results |
title_sort | inter‐laboratory comparison of pulmonary lesions induced by intratracheal instillation of nio nanoparticle in rats: histopathological examination results |
topic | Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154589/ https://www.ncbi.nlm.nih.gov/pubmed/32515878 http://dx.doi.org/10.1002/1348-9585.12117 |
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