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A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation

Although studies have reported that intestinal microbiota are associated with acute graft‐versus‐host disease (aGVHD), they lacked a satisfactory method for predicting aGVHD. We collected stool and blood samples at day 15 posttransplant from 150 patients from two centers who underwent myeloablative...

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Autores principales: Han, Lijie, Zhao, Ke, Li, Yuanyuan, Han, Haohao, Zhou, Lizhi, Ma, Ping, Fan, Zhiping, Sun, Hui, Jin, Hua, Jiang, Zhongxing, Liu, Qifa, Peng, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154648/
https://www.ncbi.nlm.nih.gov/pubmed/31605563
http://dx.doi.org/10.1111/ajt.15654
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author Han, Lijie
Zhao, Ke
Li, Yuanyuan
Han, Haohao
Zhou, Lizhi
Ma, Ping
Fan, Zhiping
Sun, Hui
Jin, Hua
Jiang, Zhongxing
Liu, Qifa
Peng, Jie
author_facet Han, Lijie
Zhao, Ke
Li, Yuanyuan
Han, Haohao
Zhou, Lizhi
Ma, Ping
Fan, Zhiping
Sun, Hui
Jin, Hua
Jiang, Zhongxing
Liu, Qifa
Peng, Jie
author_sort Han, Lijie
collection PubMed
description Although studies have reported that intestinal microbiota are associated with acute graft‐versus‐host disease (aGVHD), they lacked a satisfactory method for predicting aGVHD. We collected stool and blood samples at day 15 posttransplant from 150 patients from two centers who underwent myeloablative conditioning allogeneic hematopoietic stem cell transplantation (allo‐HSCT). Stool microbiota were detected by 16S ribosomal RNA gene sequencing; inflammatory factors and T lymphocytes were detected by multiplex immunoassays and flow cytometry, respectively. A gut microbiota score (GMS) from a LASSO (least absolute shrinkage and selection operator) model was developed and validated to predict aGVHD. In the discovery cohort, the GMS could predict II‐IV aGVHD (area under the receiver operating characteristic [ROC] curve [AUC] = 0.904, P < .0001). Furthermore, the validation model was consistent with the discovery set (AUC = 0.887, P < .0001). Regulatory T/T‐helper 17 (Treg/Th17) cells ratio in the low GMS subgroup was higher compared with the high GMS (P = .012), and the validation set is consistent with the discovery set (P = .003). In addition, high cytokine levels were associated with high GMS. In conclusion, the GMS at neutrophil engraftment could predict aGVHD, and it was a potential and novel method. The GMS was associated with the inflammatory factor and Treg/Th17 balance.
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spelling pubmed-71546482020-04-14 A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation Han, Lijie Zhao, Ke Li, Yuanyuan Han, Haohao Zhou, Lizhi Ma, Ping Fan, Zhiping Sun, Hui Jin, Hua Jiang, Zhongxing Liu, Qifa Peng, Jie Am J Transplant ORIGINAL ARTICLES Although studies have reported that intestinal microbiota are associated with acute graft‐versus‐host disease (aGVHD), they lacked a satisfactory method for predicting aGVHD. We collected stool and blood samples at day 15 posttransplant from 150 patients from two centers who underwent myeloablative conditioning allogeneic hematopoietic stem cell transplantation (allo‐HSCT). Stool microbiota were detected by 16S ribosomal RNA gene sequencing; inflammatory factors and T lymphocytes were detected by multiplex immunoassays and flow cytometry, respectively. A gut microbiota score (GMS) from a LASSO (least absolute shrinkage and selection operator) model was developed and validated to predict aGVHD. In the discovery cohort, the GMS could predict II‐IV aGVHD (area under the receiver operating characteristic [ROC] curve [AUC] = 0.904, P < .0001). Furthermore, the validation model was consistent with the discovery set (AUC = 0.887, P < .0001). Regulatory T/T‐helper 17 (Treg/Th17) cells ratio in the low GMS subgroup was higher compared with the high GMS (P = .012), and the validation set is consistent with the discovery set (P = .003). In addition, high cytokine levels were associated with high GMS. In conclusion, the GMS at neutrophil engraftment could predict aGVHD, and it was a potential and novel method. The GMS was associated with the inflammatory factor and Treg/Th17 balance. John Wiley and Sons Inc. 2019-12-12 2020-04 /pmc/articles/PMC7154648/ /pubmed/31605563 http://dx.doi.org/10.1111/ajt.15654 Text en © 2019 The Authors. American Journal of Transplantation published by Wiley Periodicals, Inc. on behalf of The American Society of Transplantation and the American Society of Transplant Surgeons This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ORIGINAL ARTICLES
Han, Lijie
Zhao, Ke
Li, Yuanyuan
Han, Haohao
Zhou, Lizhi
Ma, Ping
Fan, Zhiping
Sun, Hui
Jin, Hua
Jiang, Zhongxing
Liu, Qifa
Peng, Jie
A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title_full A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title_fullStr A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title_full_unstemmed A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title_short A gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
title_sort gut microbiota score predicting acute graft‐versus‐host disease following myeloablative allogeneic hematopoietic stem cell transplantation
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154648/
https://www.ncbi.nlm.nih.gov/pubmed/31605563
http://dx.doi.org/10.1111/ajt.15654
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