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Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications

Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could additionally serve as supports for cellular ther...

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Autores principales: Aeberhard, Pierre‐Arnaud, Grognuz, Anthony, Peneveyre, Cédric, McCallin, Shawna, Hirt‐Burri, Nathalie, Antons, Jens, Pioletti, Dominique, Raffoul, Wassim, Applegate, Lee Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154770/
https://www.ncbi.nlm.nih.gov/pubmed/31609006
http://dx.doi.org/10.1111/aor.13581
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author Aeberhard, Pierre‐Arnaud
Grognuz, Anthony
Peneveyre, Cédric
McCallin, Shawna
Hirt‐Burri, Nathalie
Antons, Jens
Pioletti, Dominique
Raffoul, Wassim
Applegate, Lee Ann
author_facet Aeberhard, Pierre‐Arnaud
Grognuz, Anthony
Peneveyre, Cédric
McCallin, Shawna
Hirt‐Burri, Nathalie
Antons, Jens
Pioletti, Dominique
Raffoul, Wassim
Applegate, Lee Ann
author_sort Aeberhard, Pierre‐Arnaud
collection PubMed
description Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could additionally serve as supports for cellular therapies to improve healing. In this study, protocols of either commonly used detergents only (SDS 1%, Triton 1%, TBP 1%, and Tween‐20 1%) or a combination of freeze/thaw (F/T) cycles with decellularization agents (NaCl 1M, ddH(2)O) were evaluated for the decellularization of horse equine superficial digital flexor tendon (SDFT) for hand flexor or extensor tendon reconstruction. Decellularization efficiency was assessed microscopically by histological staining (HE, DAPI) and DNA quantification. Macroscopical structure and biomechanical integrity of the tendon matrices were further assessed by gross observation, histological staining (SR), and mechanical testing (ultimate strain and stress, Young’s modulus, energy to failure) for select protocols. Decellularization with hypertonic NaCl 1M in association with F/T cycles produced the most robust tendon matrices, which were nontoxic after 10 days for subsequent recellularization with human fetal progenitor tendon cells (hFPTs). This standardized protocol uses a less aggressive decellularization agent than current practice, which allows subsequent reseeding with allogenic cells, therefore making them very suitable and bioengineered tendon matrices for human tendon reconstruction in the clinic.
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spelling pubmed-71547702020-04-15 Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications Aeberhard, Pierre‐Arnaud Grognuz, Anthony Peneveyre, Cédric McCallin, Shawna Hirt‐Burri, Nathalie Antons, Jens Pioletti, Dominique Raffoul, Wassim Applegate, Lee Ann Artif Organs Electronic‐only Articles Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could additionally serve as supports for cellular therapies to improve healing. In this study, protocols of either commonly used detergents only (SDS 1%, Triton 1%, TBP 1%, and Tween‐20 1%) or a combination of freeze/thaw (F/T) cycles with decellularization agents (NaCl 1M, ddH(2)O) were evaluated for the decellularization of horse equine superficial digital flexor tendon (SDFT) for hand flexor or extensor tendon reconstruction. Decellularization efficiency was assessed microscopically by histological staining (HE, DAPI) and DNA quantification. Macroscopical structure and biomechanical integrity of the tendon matrices were further assessed by gross observation, histological staining (SR), and mechanical testing (ultimate strain and stress, Young’s modulus, energy to failure) for select protocols. Decellularization with hypertonic NaCl 1M in association with F/T cycles produced the most robust tendon matrices, which were nontoxic after 10 days for subsequent recellularization with human fetal progenitor tendon cells (hFPTs). This standardized protocol uses a less aggressive decellularization agent than current practice, which allows subsequent reseeding with allogenic cells, therefore making them very suitable and bioengineered tendon matrices for human tendon reconstruction in the clinic. John Wiley and Sons Inc. 2019-11-14 2020-04 /pmc/articles/PMC7154770/ /pubmed/31609006 http://dx.doi.org/10.1111/aor.13581 Text en © 2019 The Authors. Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Electronic‐only Articles
Aeberhard, Pierre‐Arnaud
Grognuz, Anthony
Peneveyre, Cédric
McCallin, Shawna
Hirt‐Burri, Nathalie
Antons, Jens
Pioletti, Dominique
Raffoul, Wassim
Applegate, Lee Ann
Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title_full Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title_fullStr Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title_full_unstemmed Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title_short Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
title_sort efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications
topic Electronic‐only Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154770/
https://www.ncbi.nlm.nih.gov/pubmed/31609006
http://dx.doi.org/10.1111/aor.13581
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