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Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress
Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low tem...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154934/ https://www.ncbi.nlm.nih.gov/pubmed/32138235 http://dx.doi.org/10.3390/plants9030315 |
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author | Acosta-Pérez, Phamela Camacho-Zamora, Bianka Dianey Espinoza-Sánchez, Edward A. Gutiérrez-Soto, Guadalupe Zavala-García, Francisco Abraham-Juárez, María Jazmín Sinagawa-García, Sugey Ramona |
author_facet | Acosta-Pérez, Phamela Camacho-Zamora, Bianka Dianey Espinoza-Sánchez, Edward A. Gutiérrez-Soto, Guadalupe Zavala-García, Francisco Abraham-Juárez, María Jazmín Sinagawa-García, Sugey Ramona |
author_sort | Acosta-Pérez, Phamela |
collection | PubMed |
description | Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low temperature, and osmotic stress. Thus, the aim of this work was to clone and characterize at molecular level the trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes from maize and to evaluate its differential expression in maize seedlings under drought stress. To carry out this, resistant and susceptible maize lines were subjected to drought stress during 72 h. Two full-length cDNAs of TPS and one of TPP were cloned and sequenced. Then, TPS and TPP amino acid sequences were aligned with their homologs from different species, showing highly conserved domains and the same catalytic sites. Relative expression of both genes was evaluated by RT-qPCR at different time points. The expression pattern showed significant induction after 0.5 h in resistant lines and after two to four hours in susceptible plants, showing their participation in drought stress response. |
format | Online Article Text |
id | pubmed-7154934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71549342020-04-21 Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress Acosta-Pérez, Phamela Camacho-Zamora, Bianka Dianey Espinoza-Sánchez, Edward A. Gutiérrez-Soto, Guadalupe Zavala-García, Francisco Abraham-Juárez, María Jazmín Sinagawa-García, Sugey Ramona Plants (Basel) Article Maize is the most important crop around the world and it is highly sensitive to abiotic stress caused by drought, excessive salinity, and extreme temperature. In plants, trehalose has been widely studied for its role in plant adaptation to different abiotic stresses such as drought, high and low temperature, and osmotic stress. Thus, the aim of this work was to clone and characterize at molecular level the trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes from maize and to evaluate its differential expression in maize seedlings under drought stress. To carry out this, resistant and susceptible maize lines were subjected to drought stress during 72 h. Two full-length cDNAs of TPS and one of TPP were cloned and sequenced. Then, TPS and TPP amino acid sequences were aligned with their homologs from different species, showing highly conserved domains and the same catalytic sites. Relative expression of both genes was evaluated by RT-qPCR at different time points. The expression pattern showed significant induction after 0.5 h in resistant lines and after two to four hours in susceptible plants, showing their participation in drought stress response. MDPI 2020-03-03 /pmc/articles/PMC7154934/ /pubmed/32138235 http://dx.doi.org/10.3390/plants9030315 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Acosta-Pérez, Phamela Camacho-Zamora, Bianka Dianey Espinoza-Sánchez, Edward A. Gutiérrez-Soto, Guadalupe Zavala-García, Francisco Abraham-Juárez, María Jazmín Sinagawa-García, Sugey Ramona Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title_full | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title_fullStr | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title_full_unstemmed | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title_short | Characterization of Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Genes and Analysis of its Differential Expression in Maize (Zea mays) Seedlings under Drought Stress |
title_sort | characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase genes and analysis of its differential expression in maize (zea mays) seedlings under drought stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154934/ https://www.ncbi.nlm.nih.gov/pubmed/32138235 http://dx.doi.org/10.3390/plants9030315 |
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