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Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia

BACKGROUND: The spread of carbapenem-resistant A. baumannii (CrAb) is gaining worldwide attention. The spread of this pathogen is largely due to its ability to acquire various resistance genes of intrinsic and extrinsic origins that confer unpredictable susceptibility to β-lactams. The aim of this s...

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Autores principales: Rao, Mohan, Rashid, Fairuz A., Shukor, Surianti, Hashim, Rohaidah, Ahmad, Norazah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154965/
https://www.ncbi.nlm.nih.gov/pubmed/32318127
http://dx.doi.org/10.1155/2020/5021064
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author Rao, Mohan
Rashid, Fairuz A.
Shukor, Surianti
Hashim, Rohaidah
Ahmad, Norazah
author_facet Rao, Mohan
Rashid, Fairuz A.
Shukor, Surianti
Hashim, Rohaidah
Ahmad, Norazah
author_sort Rao, Mohan
collection PubMed
description BACKGROUND: The spread of carbapenem-resistant A. baumannii (CrAb) is gaining worldwide attention. The spread of this pathogen is largely due to its ability to acquire various resistance genes of intrinsic and extrinsic origins that confer unpredictable susceptibility to β-lactams. The aim of this study was to analyze β-lactamase genetic compositions of CrAb in Malaysia. METHODS: Whole-genome sequencing (WGS) was carried out on 13 CrAb isolates from clinical samples in Malaysia from 2011 to 2016. RESULTS: Endotracheal aspirate was the dominant clinical sample source (n = 6), and only one isolate was obtained from wound swab. A total of 6 sequence types (STs) of the Oxford scheme were identified, including 4 reported STs and 2 novel STs. Eleven isolates were classified into clonal complex 92 (CC92/ICII), among which ST195 and ST208 were the most prevalent STs. All 13 CrAb isolates harbored multiple β-lactamase genes. bla(OXA-23) (n = 13) and bla(OXA-66) (n = 11) were the dominant carbapenemase gene families found in these isolates. All isolates harbor bla(ADC), bla(OXA-51-like), and bla(OXA-23-like) genes. bla(TEM) (n = 7), bla(NDM-1) (n = 3), bla(CARB-8) (n = 1), and bla(PER-3) (n = 1) are amongst other β-lactamase genes found in this study. ISAba1 was found upstream to bla(OXA-23) (n = 13), bla(OXA-66) (n = 1), and bla(ADC) (n = 11). All bla(NDM-1) isolates had ISAba125 (mobile genetic element) upstream to the genes. All isolates were positive for Tn2006/2008 and Tn2009 but were negative for Tn2007. CONCLUSION: Most of the isolates were grouped under the CC92 clonal complex which belongs to international clonal lineage 2. These findings predict that carriage of carbapenem-resistant genes possibly constitutes the underlying basis of high level of international clone II prevalence. Therefore, molecular surveillance and antimicrobial stewardship are essential in implementing policies to prevent and control the spread of CrAb in hospital settings.
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spelling pubmed-71549652020-04-21 Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia Rao, Mohan Rashid, Fairuz A. Shukor, Surianti Hashim, Rohaidah Ahmad, Norazah Can J Infect Dis Med Microbiol Research Article BACKGROUND: The spread of carbapenem-resistant A. baumannii (CrAb) is gaining worldwide attention. The spread of this pathogen is largely due to its ability to acquire various resistance genes of intrinsic and extrinsic origins that confer unpredictable susceptibility to β-lactams. The aim of this study was to analyze β-lactamase genetic compositions of CrAb in Malaysia. METHODS: Whole-genome sequencing (WGS) was carried out on 13 CrAb isolates from clinical samples in Malaysia from 2011 to 2016. RESULTS: Endotracheal aspirate was the dominant clinical sample source (n = 6), and only one isolate was obtained from wound swab. A total of 6 sequence types (STs) of the Oxford scheme were identified, including 4 reported STs and 2 novel STs. Eleven isolates were classified into clonal complex 92 (CC92/ICII), among which ST195 and ST208 were the most prevalent STs. All 13 CrAb isolates harbored multiple β-lactamase genes. bla(OXA-23) (n = 13) and bla(OXA-66) (n = 11) were the dominant carbapenemase gene families found in these isolates. All isolates harbor bla(ADC), bla(OXA-51-like), and bla(OXA-23-like) genes. bla(TEM) (n = 7), bla(NDM-1) (n = 3), bla(CARB-8) (n = 1), and bla(PER-3) (n = 1) are amongst other β-lactamase genes found in this study. ISAba1 was found upstream to bla(OXA-23) (n = 13), bla(OXA-66) (n = 1), and bla(ADC) (n = 11). All bla(NDM-1) isolates had ISAba125 (mobile genetic element) upstream to the genes. All isolates were positive for Tn2006/2008 and Tn2009 but were negative for Tn2007. CONCLUSION: Most of the isolates were grouped under the CC92 clonal complex which belongs to international clonal lineage 2. These findings predict that carriage of carbapenem-resistant genes possibly constitutes the underlying basis of high level of international clone II prevalence. Therefore, molecular surveillance and antimicrobial stewardship are essential in implementing policies to prevent and control the spread of CrAb in hospital settings. Hindawi 2020-04-02 /pmc/articles/PMC7154965/ /pubmed/32318127 http://dx.doi.org/10.1155/2020/5021064 Text en Copyright © 2020 Mohan Rao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rao, Mohan
Rashid, Fairuz A.
Shukor, Surianti
Hashim, Rohaidah
Ahmad, Norazah
Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title_full Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title_fullStr Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title_full_unstemmed Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title_short Detection of Antimicrobial Resistance Genes Associated with Carbapenem Resistance from the Whole-Genome Sequence of Acinetobacter baumannii Isolates from Malaysia
title_sort detection of antimicrobial resistance genes associated with carbapenem resistance from the whole-genome sequence of acinetobacter baumannii isolates from malaysia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154965/
https://www.ncbi.nlm.nih.gov/pubmed/32318127
http://dx.doi.org/10.1155/2020/5021064
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