Cargando…
The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population
Lack of protein-coding capacity is a main characteristic of long noncoding RNAs (lncRNAs) which, as molecular biomarkers, have found a novel pharmacological application in cancer and are reported to be important regulators of gene expression. H19 is reportedly involved in cancer progression and tumo...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154967/ https://www.ncbi.nlm.nih.gov/pubmed/32337223 http://dx.doi.org/10.1155/2020/1634252 |
_version_ | 1783521933509263360 |
---|---|
author | Ghapanchi, Jannan Ranjbar, Zahra Mokhtari, Mohammad Javad Koohpeima, Fatemeh Derakhshan, Maryam Khademi, Bijan Ghaderi, Hamid Sheikhbahaei, Shamsoddinali Aliabadi, Ehsan |
author_facet | Ghapanchi, Jannan Ranjbar, Zahra Mokhtari, Mohammad Javad Koohpeima, Fatemeh Derakhshan, Maryam Khademi, Bijan Ghaderi, Hamid Sheikhbahaei, Shamsoddinali Aliabadi, Ehsan |
author_sort | Ghapanchi, Jannan |
collection | PubMed |
description | Lack of protein-coding capacity is a main characteristic of long noncoding RNAs (lncRNAs) which, as molecular biomarkers, have found a novel pharmacological application in cancer and are reported to be important regulators of gene expression. H19 is reportedly involved in cancer progression and tumorigenesis. One of the most common types of head and neck cancers is oral squamous cell carcinoma (OSCC). The main objective of the present study was to evaluate the correlation of OSCC susceptibility with H19 gene in an Iranian population. This research was performed on 400 subjects of both sexes referred to the Namazi Hospital affiliated with the Shiraz University of Medical Sciences (SUMS). Individuals aged 15-88 years were divided into two groups: pathologically diagnosed patients with new-onset OSCC and healthy controls. After written and informed consent was obtained from the individuals, genomic DNA was extracted. The tetra-primer ARMS-PCR technique was performed for DNA genotyping by the use of specific primer pairs. The susceptibility of OSCC and H19 gene polymorphism sites was further analyzed (rs217727 and rs2107425). The allele and genotype frequencies of H19 rs2107425 polymorphism were similar between OSCC cases and controls. The H19 rs217727T allele frequency was significantly higher in OSCC cases (P = 0.002), and the polymorphism of H19 rs217727 was associated with OSCC susceptibility in the codominant (OR = 6.04, 95%CI = 1.70 − 21.42, P = 0.001 for TT genotype), dominant (OR = 1.62, 95%CI = 1.08 − 2.43, P = 0.01), and recessive (OR = 5.32, 95%CI = 1.51 − 18.69, P = 0.003) models. This study showed that rs217727 and OSCC susceptibility were statistically correlated in the Iranian population. |
format | Online Article Text |
id | pubmed-7154967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-71549672020-04-24 The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population Ghapanchi, Jannan Ranjbar, Zahra Mokhtari, Mohammad Javad Koohpeima, Fatemeh Derakhshan, Maryam Khademi, Bijan Ghaderi, Hamid Sheikhbahaei, Shamsoddinali Aliabadi, Ehsan Biomed Res Int Research Article Lack of protein-coding capacity is a main characteristic of long noncoding RNAs (lncRNAs) which, as molecular biomarkers, have found a novel pharmacological application in cancer and are reported to be important regulators of gene expression. H19 is reportedly involved in cancer progression and tumorigenesis. One of the most common types of head and neck cancers is oral squamous cell carcinoma (OSCC). The main objective of the present study was to evaluate the correlation of OSCC susceptibility with H19 gene in an Iranian population. This research was performed on 400 subjects of both sexes referred to the Namazi Hospital affiliated with the Shiraz University of Medical Sciences (SUMS). Individuals aged 15-88 years were divided into two groups: pathologically diagnosed patients with new-onset OSCC and healthy controls. After written and informed consent was obtained from the individuals, genomic DNA was extracted. The tetra-primer ARMS-PCR technique was performed for DNA genotyping by the use of specific primer pairs. The susceptibility of OSCC and H19 gene polymorphism sites was further analyzed (rs217727 and rs2107425). The allele and genotype frequencies of H19 rs2107425 polymorphism were similar between OSCC cases and controls. The H19 rs217727T allele frequency was significantly higher in OSCC cases (P = 0.002), and the polymorphism of H19 rs217727 was associated with OSCC susceptibility in the codominant (OR = 6.04, 95%CI = 1.70 − 21.42, P = 0.001 for TT genotype), dominant (OR = 1.62, 95%CI = 1.08 − 2.43, P = 0.01), and recessive (OR = 5.32, 95%CI = 1.51 − 18.69, P = 0.003) models. This study showed that rs217727 and OSCC susceptibility were statistically correlated in the Iranian population. Hindawi 2020-03-31 /pmc/articles/PMC7154967/ /pubmed/32337223 http://dx.doi.org/10.1155/2020/1634252 Text en Copyright © 2020 Jannan Ghapanchi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ghapanchi, Jannan Ranjbar, Zahra Mokhtari, Mohammad Javad Koohpeima, Fatemeh Derakhshan, Maryam Khademi, Bijan Ghaderi, Hamid Sheikhbahaei, Shamsoddinali Aliabadi, Ehsan The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title | The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title_full | The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title_fullStr | The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title_full_unstemmed | The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title_short | The LncRNA H19 rs217727 Polymorphism Is Associated with Oral Squamous Cell Carcinoma Susceptibility in Iranian Population |
title_sort | lncrna h19 rs217727 polymorphism is associated with oral squamous cell carcinoma susceptibility in iranian population |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7154967/ https://www.ncbi.nlm.nih.gov/pubmed/32337223 http://dx.doi.org/10.1155/2020/1634252 |
work_keys_str_mv | AT ghapanchijannan thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT ranjbarzahra thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT mokhtarimohammadjavad thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT koohpeimafatemeh thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT derakhshanmaryam thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT khademibijan thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT ghaderihamid thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT sheikhbahaeishamsoddinali thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT aliabadiehsan thelncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT ghapanchijannan lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT ranjbarzahra lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT mokhtarimohammadjavad lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT koohpeimafatemeh lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT derakhshanmaryam lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT khademibijan lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT ghaderihamid lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT sheikhbahaeishamsoddinali lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation AT aliabadiehsan lncrnah19rs217727polymorphismisassociatedwithoralsquamouscellcarcinomasusceptibilityiniranianpopulation |