Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography

An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [(11)C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabe...

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Detalles Bibliográficos
Autores principales: Milicevic Sephton, Selena, Miklovicz, Tunde, Russell, Joseph J., Doke, Aniruddha, Li, Lei, Boros, Istvan, Aigbirhio, Franklin I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Protocols and Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155065/
https://www.ncbi.nlm.nih.gov/pubmed/32027052
http://dx.doi.org/10.1002/jlcr.3828
Descripción
Sumario:An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [(11)C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabelling precursor, and the desired radiolabelled product was isolated in 11 ± 2% (n = 7) nondecay corrected radiochemical yield and formulated as a 10% EtOH solution in saline with molar activities of 20 to 100 GBq/μmol. The method was based upon the palladium(0)‐mediated Suzuki cross‐coupling reaction and [(11)C]CH(3)I as a radiolabelling synthon. The isolated product was cGMP compliant as demonstrated by the results of quality control analysis.

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