Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography

An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [(11)C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabe...

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Autores principales: Milicevic Sephton, Selena, Miklovicz, Tunde, Russell, Joseph J., Doke, Aniruddha, Li, Lei, Boros, Istvan, Aigbirhio, Franklin I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Protocols and Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155065/
https://www.ncbi.nlm.nih.gov/pubmed/32027052
http://dx.doi.org/10.1002/jlcr.3828
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author Milicevic Sephton, Selena
Miklovicz, Tunde
Russell, Joseph J.
Doke, Aniruddha
Li, Lei
Boros, Istvan
Aigbirhio, Franklin I.
author_facet Milicevic Sephton, Selena
Miklovicz, Tunde
Russell, Joseph J.
Doke, Aniruddha
Li, Lei
Boros, Istvan
Aigbirhio, Franklin I.
author_sort Milicevic Sephton, Selena
collection PubMed
description An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [(11)C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabelling precursor, and the desired radiolabelled product was isolated in 11 ± 2% (n = 7) nondecay corrected radiochemical yield and formulated as a 10% EtOH solution in saline with molar activities of 20 to 100 GBq/μmol. The method was based upon the palladium(0)‐mediated Suzuki cross‐coupling reaction and [(11)C]CH(3)I as a radiolabelling synthon. The isolated product was cGMP compliant as demonstrated by the results of quality control analysis.
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spelling pubmed-71550652020-04-15 Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography Milicevic Sephton, Selena Miklovicz, Tunde Russell, Joseph J. Doke, Aniruddha Li, Lei Boros, Istvan Aigbirhio, Franklin I. J Labelled Comp Radiopharm Protocols and Methods An automated radiosynthesis of carbon‐11 positron emission tomography radiotracer [(11)C]UCB‐J for imaging the synaptic density biomarker synaptic vesicle glycoprotein SV2A was established using Synthra RNPlus synthesizer. Commercially available trifluoroborate UCB‐J analogue was used as a radiolabelling precursor, and the desired radiolabelled product was isolated in 11 ± 2% (n = 7) nondecay corrected radiochemical yield and formulated as a 10% EtOH solution in saline with molar activities of 20 to 100 GBq/μmol. The method was based upon the palladium(0)‐mediated Suzuki cross‐coupling reaction and [(11)C]CH(3)I as a radiolabelling synthon. The isolated product was cGMP compliant as demonstrated by the results of quality control analysis. John Wiley and Sons Inc. 2020-02-06 2020-03 /pmc/articles/PMC7155065/ /pubmed/32027052 http://dx.doi.org/10.1002/jlcr.3828 Text en © 2020 The Authors. Journal of Labelled Compounds and Radiopharmaceuticals published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Protocols and Methods
Milicevic Sephton, Selena
Miklovicz, Tunde
Russell, Joseph J.
Doke, Aniruddha
Li, Lei
Boros, Istvan
Aigbirhio, Franklin I.
Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title_full Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title_fullStr Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title_full_unstemmed Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title_short Automated radiosynthesis of [(11)C]UCB‐J for imaging synaptic density by positron emission tomography
title_sort automated radiosynthesis of [(11)c]ucb‐j for imaging synaptic density by positron emission tomography
topic Protocols and Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155065/
https://www.ncbi.nlm.nih.gov/pubmed/32027052
http://dx.doi.org/10.1002/jlcr.3828
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