Reverse-genetics studies of lncRNAs—what we have learnt and paths forward

Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigor...

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Detalles Bibliográficos
Autores principales: Gao, Fan, Cai, Ye, Kapranov, Philipp, Xu, Dongyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155256/
https://www.ncbi.nlm.nih.gov/pubmed/32290841
http://dx.doi.org/10.1186/s13059-020-01994-5
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author Gao, Fan
Cai, Ye
Kapranov, Philipp
Xu, Dongyang
author_facet Gao, Fan
Cai, Ye
Kapranov, Philipp
Xu, Dongyang
author_sort Gao, Fan
collection PubMed
description Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigorous debate. Here we review the evidence for and against biological functionalities of lncRNAs and attempt to arrive at potential modes of lncRNA functionality that would reconcile the contradictory conclusions. Finally, we discuss different strategies of phenotypic analyses that could be used to investigate such modes of lncRNA functionality.
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spelling pubmed-71552562020-04-20 Reverse-genetics studies of lncRNAs—what we have learnt and paths forward Gao, Fan Cai, Ye Kapranov, Philipp Xu, Dongyang Genome Biol Review Long non-coding RNAs (lncRNAs) represent a major fraction of the transcriptome in multicellular organisms. Although a handful of well-studied lncRNAs are broadly recognized as biologically meaningful, the fraction of such transcripts out of the entire collection of lncRNAs remains a subject of vigorous debate. Here we review the evidence for and against biological functionalities of lncRNAs and attempt to arrive at potential modes of lncRNA functionality that would reconcile the contradictory conclusions. Finally, we discuss different strategies of phenotypic analyses that could be used to investigate such modes of lncRNA functionality. BioMed Central 2020-04-14 /pmc/articles/PMC7155256/ /pubmed/32290841 http://dx.doi.org/10.1186/s13059-020-01994-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Review
Gao, Fan
Cai, Ye
Kapranov, Philipp
Xu, Dongyang
Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_full Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_fullStr Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_full_unstemmed Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_short Reverse-genetics studies of lncRNAs—what we have learnt and paths forward
title_sort reverse-genetics studies of lncrnas—what we have learnt and paths forward
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155256/
https://www.ncbi.nlm.nih.gov/pubmed/32290841
http://dx.doi.org/10.1186/s13059-020-01994-5
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