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Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission

Objective: Recent studies on double negative B cells (DN B cells) suggested that they have potential pathogenic roles in systemic lupus erythematosus (SLE). This study aimed to determine the circulating DN B cells in SLE patients and analyzed the clinical significance of this cell subset. Methods: F...

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Autores principales: You, Xujie, Zhang, Ruijun, Shao, Miao, He, Jing, Chen, Jiali, Liu, Jiajia, Zhang, Xia, Liu, Xu, Jia, Rulin, Sun, Xiaolin, Li, Zhanguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155774/
https://www.ncbi.nlm.nih.gov/pubmed/32318574
http://dx.doi.org/10.3389/fmed.2020.00085
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author You, Xujie
Zhang, Ruijun
Shao, Miao
He, Jing
Chen, Jiali
Liu, Jiajia
Zhang, Xia
Liu, Xu
Jia, Rulin
Sun, Xiaolin
Li, Zhanguo
author_facet You, Xujie
Zhang, Ruijun
Shao, Miao
He, Jing
Chen, Jiali
Liu, Jiajia
Zhang, Xia
Liu, Xu
Jia, Rulin
Sun, Xiaolin
Li, Zhanguo
author_sort You, Xujie
collection PubMed
description Objective: Recent studies on double negative B cells (DN B cells) suggested that they have potential pathogenic roles in systemic lupus erythematosus (SLE). This study aimed to determine the circulating DN B cells in SLE patients and analyzed the clinical significance of this cell subset. Methods: Fifty-seven SLE patients and fifty healthy controls (HCs) were recruited in this study. Among the 57 SLE patients, 25 had lupus nephritis (LN). All patients were followed up for 24 weeks. Peripheral B cell subsets were analyzed by flow cytometry. Results: DN B cells were significantly elevated in the SLE patients, especially in the patients with LN (p < 0.01). DN B showed a positive correlation with 24-h urine protein excretion (24 h-UPE) levels (r = 0.444, p = 0.034) in LN patients, and inversely correlated with evaluated glomerular filtration rate (eGFR) (r = −0.351, p = 0.011). DN B cells had a positive correlation with plasma cells (r = 0.484, p < 0.001) and memory B cells (r = 0.703, p < 0.001). After treatment, decreased DN B cells were associated with LN alleviation (p = 0.002). In the follow-up, the remission rate of LN patients with decreased DN B cells was significantly higher than LN patients with increased DN B cells (83.33 vs. 25.00%, p = 0.030) at week 24. Conclusions: This study suggests that the peripheral DN B cells are positively correlated with the severity of renal damage in LN patients and may potentially be used as a prognostic marker in LN.
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spelling pubmed-71557742020-04-21 Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission You, Xujie Zhang, Ruijun Shao, Miao He, Jing Chen, Jiali Liu, Jiajia Zhang, Xia Liu, Xu Jia, Rulin Sun, Xiaolin Li, Zhanguo Front Med (Lausanne) Medicine Objective: Recent studies on double negative B cells (DN B cells) suggested that they have potential pathogenic roles in systemic lupus erythematosus (SLE). This study aimed to determine the circulating DN B cells in SLE patients and analyzed the clinical significance of this cell subset. Methods: Fifty-seven SLE patients and fifty healthy controls (HCs) were recruited in this study. Among the 57 SLE patients, 25 had lupus nephritis (LN). All patients were followed up for 24 weeks. Peripheral B cell subsets were analyzed by flow cytometry. Results: DN B cells were significantly elevated in the SLE patients, especially in the patients with LN (p < 0.01). DN B showed a positive correlation with 24-h urine protein excretion (24 h-UPE) levels (r = 0.444, p = 0.034) in LN patients, and inversely correlated with evaluated glomerular filtration rate (eGFR) (r = −0.351, p = 0.011). DN B cells had a positive correlation with plasma cells (r = 0.484, p < 0.001) and memory B cells (r = 0.703, p < 0.001). After treatment, decreased DN B cells were associated with LN alleviation (p = 0.002). In the follow-up, the remission rate of LN patients with decreased DN B cells was significantly higher than LN patients with increased DN B cells (83.33 vs. 25.00%, p = 0.030) at week 24. Conclusions: This study suggests that the peripheral DN B cells are positively correlated with the severity of renal damage in LN patients and may potentially be used as a prognostic marker in LN. Frontiers Media S.A. 2020-04-07 /pmc/articles/PMC7155774/ /pubmed/32318574 http://dx.doi.org/10.3389/fmed.2020.00085 Text en Copyright © 2020 You, Zhang, Shao, He, Chen, Liu, Zhang, Liu, Jia, Sun and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
You, Xujie
Zhang, Ruijun
Shao, Miao
He, Jing
Chen, Jiali
Liu, Jiajia
Zhang, Xia
Liu, Xu
Jia, Rulin
Sun, Xiaolin
Li, Zhanguo
Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title_full Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title_fullStr Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title_full_unstemmed Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title_short Double Negative B Cell Is Associated With Renal Impairment in Systemic Lupus Erythematosus and Acts as a Marker for Nephritis Remission
title_sort double negative b cell is associated with renal impairment in systemic lupus erythematosus and acts as a marker for nephritis remission
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155774/
https://www.ncbi.nlm.nih.gov/pubmed/32318574
http://dx.doi.org/10.3389/fmed.2020.00085
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